Kcnn4 (NM_008433) Mouse Tagged ORF Clone

CAT#: MR206777

Kcnn4 (Myc-DDK-tagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 (Kcnn4), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnn4
• Synonyms: IK1; IKCA1; KCa3.1; KCA4; mIKCa1; SK4; SKCas
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206777 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGGGAGCTGGTGACTGGCCTGGGGGCCCTGAGACGGAGAAAGCGCCTGCTGGAGCAGGAGAAGA
  GGGTGGCCGGCTGGGCGTTGGTGCTGGCGGGAACTGGCATCGGACTCATGGTTCTGCACGCTGAGATGTT
  GTGGTTCCTGGGCTGCAAGTGGGTGCTGTACCTGCTCCTGGTTAAGTGTTTGATCACCCTGTCCACTGCC
  TTCCTCCTTTGTCTTATTGTGGTCTTCCATGCCAAGGAGGTCCAGCTGTTCATGACTGACAACGGGCTCC
  GGGACTGGCGCGTGGCGCTGACCCGGCGGCAGGTGGCGCAGATCCTGCTGGAGCTGTTGGTGTGCGGGGT
  GCACCCGGTGCCCCTACGGAGCCCGCACTGCGCCCTGGCGGGGGAGGCCACCGACGCGCAGCCCTGGCCG
  GGTTTCCTGGGCGAAGGCGAGGCGTTGCTGTCCCTGGCCATGCTCCTGCGTCTCTACCTGGTGCCCCGCG
  CGGTGCTGCTGCGCAGCGGGGTCCTGCTCAACGCGTCCTACCGCAGCATCGGGGCGCTCAACCAAGTCCG
  CTTCCGCCACTGGTTCGTGGCCAAGCTGTACATGAACACGCACCCGGGTCGCCTGCTGCTGGGCCTCACG
  CTGGGTCTCTGGCTCACCACAGCTTGGGTGCTGTCTGTGGCTGAGAGGCAGGCTGTCAATGCCACGGGGC
  ACCTCACAGACACACTGTGGCTGATTCCGATCACATTCCTGACCATTGGCTATGGGGACGTGGTACCTGG
  CACCATGTGGGGCAAGATTGTCTGCCTGTGCACCGGAGTCATGGGGGTCTGCTGCACAGCTCTCCTGGTG
  GCTGTGGTGGCTCGGAAGCTGGAGTTCAACAAGGCGGAGAAACACGTGCACAACTTCATGATGGACATCC
  ATTATGCCAAAGAGATGAAGGAGTCAGCGGCGCGGCTGCTGCAGGAAGCCTGGATGTACTACAAGCACAC
  TCGAAGGAAGGACTCCCGGGCTGCCCGCAGACATCAGCGCAAGATGCTGGCCGCCATCCACACGTTCCGC
  CAGGTACGGCTGAAACACCGGAAGCTCCGGGAACAAGTGAATTCCATGGTGGACATCTCCAAGATGCACA
  TGATCCTGTGCGACCTGCAGCTGGGTCTCAGCTCCTCGCACCGTGCCCTGGAGAAGAGAATCGACGGTCT
  GGCAGGAAAGCTGGATGCCCTGACAGAGCTGCTCGGCACTGCCCTGCAGCAACAGCAGCTACCAGAACCC
  AGCCAGGAGGCCACA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206777 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGELVTGLGALRRRKRLLEQEKRVAGWALVLAGTGIGLMVLHAEMLWFLGCKWVLYLLLVKCLITLSTA
  FLLCLIVVFHAKEVQLFMTDNGLRDWRVALTRRQVAQILLELLVCGVHPVPLRSPHCALAGEATDAQPWP
  GFLGEGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLT
  LGLWLTTAWVLSVAERQAVNATGHLTDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLV
  AVVARKLEFNKAEKHVHNFMMDIHYAKEMKESAARLLQEAWMYYKHTRRKDSRAARRHQRKMLAAIHTFR
  QVRLKHRKLREQVNSMVDISKMHMILCDLQLGLSSSHRALEKRIDGLAGKLDALTELLGTALQQQQLPEP
  SQEAT
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008433
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008433.1, NM_008433.2, NM_008433.3, NM_008433.4, NP_032459.2
• RefSeq Size: 1956 bp
• RefSeq ORF: 1278 bp
• Locus ID: 16534
• MW: 47.8 kDa