Plekha2 (NM_031257) Mouse Tagged ORF Clone

CAT#: MR206779

Plekha2 (Myc-DDK-tagged) - Mouse pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2 (Plekha2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekha2
• Synonyms: 6430512N22Rik; AI851530; AU041791; TAPP2; W91773
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206779 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTATGTGGATCGGCAGAACCGAATCTGTGGGTTTCTGGATATTGAAGACAATGAGAACAGTGGCA
  AATTCCTCCGGAGATACTTTATCCTGGATACCCAGGCCAACTGCCTCCTCTGGTACATGGACAATCCCCA
  GAACCTGGCCGTTGGGGCAGGAGCTGTCGGATCTCTGCAGCTGACCTACATCTCGAAGGTGAGCATAGCT
  ACCCCAAAGCAGAAACCTAAAACACCATTCTGCTTCGTTATCAATGCCCTGTCTCAGAGATATTTTCTTC
  AAGCCAATGACCAGAAAGATCTGAAGGACTGGGTAGAAGCCTTGAACCAAGCCAGCAAGATCACTGTACC
  CAAAGCTGGGACAGTACCCTTGGCCACAGAAGTTCTCAAAAACTTAACAGCTCCTCCCACCCTAGAGAAG
  AAGCCGCAGGTGGCCTACAAGACTGAGATCATCGGGGGTGTGGTGGTACAAACGCCTATCAGCCAGAACG
  GTGGGGATGGGCAGGAAGGGTGCGAGCCAGGGACTCACGCCTTCCTGCGAAGGTCTCAGAGCTACATCCC
  CACGTCAGGCTGCCGCCCTTCCACTGGGCCTCCCCTCATTAAGAGTGGCTACTGTGTGAAGCAAGGGAAT
  GTGCGGAAGAGTTGGAAACGACGCTTCTTTGCCCTCGATGACTTTACCATCTGCTACTTCAAGTGTGAGC
  AGGACAGAGAGCCTCTGCGTACCATACCGCTCAAGGATGTTCTCAAGACTCACGAGTGTCTGGTCAAGTC
  TGGTGATCTCTTAATGAGGGACAACCTGTTTGAAATCATAACCACCTCCAGGACGTTCTACGTACAGGCG
  GACAGCCCTGAGGACATGCACAGCTGGATCGAGGGGATTGGAGCAGCTGTCCAGGCTCTGAAGTGCCACC
  CTAGGGAGCCGTCCTTTTCAAGGTCCATTTCTTTGACTCGACCTGGAAGTTCTACCCTTACAAGCGCGCC
  TAACTCCATCTTGTCAAGAAGGCGGCCACCAGCAGAAGAGAAAAGAGGTCTCTGTAAGGCCCCTTCGGTG
  GCCTCCTCCTGGCAACCCTGGACACCTGTCCCCCAGGCTGAGGAAAAGCCGTTGTCGGTGGAGCATGCTC
  CAGAGGACTCTCTATTCATGCCTAACCCTGGGGAGAGCACAGCTACAGGGGTGCTGGCAAGTTCTCGAGT
  CAGGCACAGGTCGGAGCCCCAGCACCCCAAGGAGAAGCCATTTGTATTCAACCTTGATGACGAAAACATA
  CGAACCTCTGATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206779 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPYVDRQNRICGFLDIEDNENSGKFLRRYFILDTQANCLLWYMDNPQNLAVGAGAVGSLQLTYISKVSIA
  TPKQKPKTPFCFVINALSQRYFLQANDQKDLKDWVEALNQASKITVPKAGTVPLATEVLKNLTAPPTLEK
  KPQVAYKTEIIGGVVVQTPISQNGGDGQEGCEPGTHAFLRRSQSYIPTSGCRPSTGPPLIKSGYCVKQGN
  VRKSWKRRFFALDDFTICYFKCEQDREPLRTIPLKDVLKTHECLVKSGDLLMRDNLFEIITTSRTFYVQA
  DSPEDMHSWIEGIGAAVQALKCHPREPSFSRSISLTRPGSSTLTSAPNSILSRRRPPAEEKRGLCKAPSV
  ASSWQPWTPVPQAEEKPLSVEHAPEDSLFMPNPGESTATGVLASSRVRHRSEPQHPKEKPFVFNLDDENI
  RTSDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031257
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031257.1, NM_031257.2, NM_031257.3, NP_112547.1
• RefSeq Size: 4992 bp
• RefSeq ORF: 1278 bp
• Locus ID: 83436
• MW: 47.4 kDa