Islr (NM_012043) Mouse Tagged ORF Clone

CAT#: MR206818

Islr (Myc-DDK-tagged) - Mouse immunoglobulin superfamily containing leucine-rich repeat (Islr), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Islr
• Synonyms: AW553146; Meflin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206818 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCACTGTGCCTGCTTTGTTGGGCTGTTCTCCTGAACCTGGTGCGAGCTTGTCCAGAGCCCTGTG
  ACTGTGGGGAGAAGTATGGCTTCCAGATTGCAGACTGTGCCTATCGTGACCTAGAGGGTGTGCCACCGGG
  CTTCCCGGCCAATGTGACCACACTGAGCCTGTCAGCCAACAGGCTGCCAGGCCTGCCGGAGGGAGCCTTC
  AGGGAAGTGCCCCTATTGCAGTCGCTGTGGCTGGCACACAATGAGATCCGCTCGGTGGCTATTGGTGCTC
  TGGCCCCTCTGAGCCATCTCAAGAGTCTGGACCTCAGCCACAACCTCCTCTCTGAGTTTGCCTGGAGCGA
  CCTGCACAACCTCAGCGCTCTGCAGCTGCTCAAGATGGACAGCAACGAGTTAGCCTTCATCCCTCGGGAC
  GCCTTTAGCAGCCTCAGCGCCCTGCGTTCCCTGCAGCTCAACCACAACCGCCTGCACGCGTTGGCCGAGG
  GCACCTTCGCACCGCTCACCGCGCTGTCCCACTTGCAGATCAATGACAACCCTTTCGACTGCACCTGTGG
  CATCGTGTGGTTCAAGACATGGGCCCTGGCCTCAGCGGTGTCTATTCCAGAACAGGACAACATTGCCTGT
  ACTACACCCCACGTCCTGAAGGGTATCCCACTAGGCCGCCTGCCACCCCTGCCCTGCTCAGCTCCCTCAG
  TGCAACTAAGCTACCAGCCCAGCCAAGATGGAGCAGAGCTACGGCCTGGCTTCGTGCTGGCACTCCACTG
  TGACGTGGATGGACAGCCAGTCCCCCAGCTCCATTGGCACATTCACACCCCGGGCGGCACGGTGGAGATC
  GCCAGTCCTAATGTAGGCACTGATGGACGTGCCCTGCCTGGTGCCCTTGCAACCAGTGGGCAGCCACGCT
  TCCAGGCCTTTGCCAATGGCAGCCTGCTTATCCCTGACTTTGGCAAGCTGGAGGAGGGCACCTATAGCTG
  CCTGGCCACCAATGAGCTAGGCAGTGCCGAAAGCTCTGTAAATGTGGCATTGGCCACCCCAGGTGAGGGG
  GGAGAGGATGCTGTGGGGCACAAGTTCCATGGCAAAGCAGTGGAGGGCAAGGGCTGCTATACGGTTGACA
  ACGAGGTACAGCCATCCGGACCGGAAGACAACGTGGTTATCATTTACCTCAGCCGTGCTGGGCCCCCAGA
  AGCTGCAATAGCAGCAGACGGGAGGCCTGCGCAGCAGTTCTCTGGCATACTTCTGCTAGGCCAAAGCCTG
  CTTGTTCTCTCCTTTTTCTACTTC
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206818 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRALCLLCWAVLLNLVRACPEPCDCGEKYGFQIADCAYRDLEGVPPGFPANVTTLSLSANRLPGLPEGAF
  REVPLLQSLWLAHNEIRSVAIGALAPLSHLKSLDLSHNLLSEFAWSDLHNLSALQLLKMDSNELAFIPRD
  AFSSLSALRSLQLNHNRLHALAEGTFAPLTALSHLQINDNPFDCTCGIVWFKTWALASAVSIPEQDNIAC
  TTPHVLKGIPLGRLPPLPCSAPSVQLSYQPSQDGAELRPGFVLALHCDVDGQPVPQLHWHIHTPGGTVEI
  ASPNVGTDGRALPGALATSGQPRFQAFANGSLLIPDFGKLEEGTYSCLATNELGSAESSVNVALATPGEG
  GEDAVGHKFHGKAVEGKGCYTVDNEVQPSGPEDNVVIIYLSRAGPPEAAIAADGRPAQQFSGILLLGQSL
  LVLSFFYF
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012043
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012043.1, NM_012043.2, NM_012043.3, NM_012043.4, NP_036173.1
• RefSeq Size: 2243 bp
• RefSeq ORF: 1287 bp
• Locus ID: 26968
• MW: 45.6 kDa