Flot1 (NM_008027) Mouse Tagged ORF Clone

CAT#: MR206823

Flot1 (Myc-DDK-tagged) - Mouse flotillin 1 (Flot1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flot1
• Synonyms: reggie-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206823 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTCCGGGTTCTGCAGGAGCCCCCCAGTCATGG
  TGGCCGGAGGCCGAGTGTTTGTCCTACCCTGCATTCAGCAAATCCAGAGGATCTCTCTCAACACACTGAC
  CCTCAATGTCAAGAGTGAAAAGGTTTATACCCGCCACGGGGTCCCCATCTCAGTCACGGGCATTGCCCAG
  GTGAAAATCCAGGGCCAGAACAAGGAGATGCTGGCAGCTGCCTGCCAGATGTTCCTGGGGAAGACAGAGG
  CGGAGATTGCCCACATTGCCCTGGAGACACTGGAAGGCCACCAGAGGGCAATCATGGCCCACATGACTGT
  GGAGGAGATCTATAAGGATAGACAGAAATTCTCAGAGCAGGTTTTCAAAGTGGCCTCCTCCGACCTGGTC
  AACATGGGTATCAGCGTGGTTAGCTACACTTTGAAGGATATTCATGATGACCAGGATTACTTACACTCGT
  TAGGAAAGGCTCGCACGGCACAAGTCCAAAAAGATGCTCGGATTGGCGAAGCAGAGGCCAAGAGAGATGC
  CGGGATACGGGAGGCTAAGGCCAAGCAGGAGAAGGTATCTGCACAGTGCCTCAGTGAAATAGAGATGGCC
  AAGGCACAGAGGGACTATGAGCTGAAGAAAGCCACCTACGACATCGAGGTCAACACCCGGCGAGCACAGG
  CTGACCTGGCCTATCAGCTGCAGGTGGCCAAGACGAAGCAGCAGATCGAGGAGCAGCGGGTGCAGGTGCA
  GGTGGTTGAGCGCGCCCAGCAGGTGGCAGTGCAGGAGCAGGAGATCGCCCGGCGCGAGAAGGAGTTGGAG
  GCTCGCGTGCGCAAGCCAGCAGAGGCTGAGCGCTACAGGCTGGAGCGCCTGGCGGAAGCAGAGAAGGCCC
  AGCTGATCATGCAGGCAGAAGCCGAAGCTGAGTCTGTGCGGATGCGAGGGGAAGCTGAGGCCTTTGCTAT
  AGGTGCCCGCGCTCGTGCTGAGGCTGAGCAAATGGCCAAGAAGGCAGAAGCCTTCCAGATGTACCAGGAG
  GCCGCCCAGCTGGACATGCTGCTGGAGAAACTGCCCCAGGTGGCAGAAGAGATCAGTGGTCCCCTGACCT
  CAGCCAATAAGATCACGCTGGTGTCTAGTGGAAGCGGAACCATGGGGGCAGCCAAAGTTACTGGGGAAGT
  ACTGGACATCTTGAGCCGCCTGCCGGAGAGTGTGGAGAGGCTCACAGGCGTCAGCATCTCCCAGGTAAAT
  CACAACAAGCCTTTAAGGACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206823 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQ
  VKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLV
  NMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQCLSEIEMA
  KAQRDYELKKATYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELE
  ARVRKPAEAERYRLERLAEAEKAQLIMQAEAEAESVRMRGEAEAFAIGARARAEAEQMAKKAEAFQMYQE
  AAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILSRLPESVERLTGVSISQVN
  HNKPLRTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008027
• ORF Size: 1287 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008027.1, NM_008027.2, NP_032053.1
• RefSeq Size: 1694
• RefSeq ORF: 1287
• Locus ID: 14251
• MW: 47.5 kDa
• Gene Summary: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. [UniProtKB/Swiss-Prot Function]