Smyd3 (NM_027188) Mouse Tagged ORF Clone

CAT#: MR206824

Smyd3 (Myc-DDK-tagged) - Mouse SET and MYND domain containing 3 (Smyd3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smyd3
• Synonyms: 2410008A19Rik; Zmynd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206824 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCACTGAAGGTGGAAAAGTTCACGACTGCCAACCGGGGAAACGGGCTGCGTGCTGTGGCTCCAC
  TGCGCCCCGGGGAGCTGCTCTTCCGCTCCGACCCCTTGGCTTACACTGTGTGCAAGGGGAGTCGTGGCGT
  AGTCTGTGATCGCTGCCTACTGGGGAAGGAGAAGCTGATGCGTTGTTCTCAATGCCGAATTGCCAAATAC
  TGCAGTGCCAAGTGTCAGAAAAAAGCCTGGCCGGACCACAGACGGGAATGCAGTTGTCTTAAAAGCTGCA
  AGCCCAGATATCCGCCCGACTCCGTGAGACTTCTGGGCAGGGTTATCGTCAAGCTGATGGATGAAAAGCC
  CTCAGAGTCAGAGAAACTTTATTCATTTTATGATCTGGAGTCCAATATTAGCAAACTCACTGAAGATAAG
  AAAGAGGGCCTGAGGCAACTTGCAATGACTTTTCAACATTTCATGAGAGAGGAGATCCAGGATGCGTCTC
  AGCTGCCGCCTTCTTTTGACCTTTTTGAAGCCTTTGCAAAAGTGATCTGCAATTCGTTCACTATCTGCAA
  CGCGGAGATGCAGGAGGTCGGCGTTGGCCTGTACCCCAGTATGTCTTTGCTGAATCACAGCTGTGACCCC
  AACTGCTCCATCGTATTCAACGGGCCCCACCTCTTACTGCGTGCAGTGCGGGAAATTGAAGCAGGAGAGG
  AGCTCACCATCTGCTACCTGGACATGCTGATGACCAGCGAGGAACGCCGGAAGCAGCTGAGGGACCAGTA
  CTGCTTTGAGTGTGACTGCATCCGATGCCAAACCCAGGACAAGGATGCTGACATGCTAACGGGTGACGAG
  CAAATATGGAAGGAGGTTCAAGAGTCGCTGAAGAAAATCGAAGAGCTGAAGGCGCACTGGAAGTGGGAGC
  AGGTTCTGGCGCTGTGCCAGGCGATCATAAACAGCAATTCCAACCGGCTTCCCGACATCAACATCTACCA
  GCTGAAGGTGCTCGACTGTGCCATGGATGCCTGCATCAACCTGGGGATGCTGGAGGAGGCGTTGTTCTAC
  GCCATGCGCACCATGGAGCCGTACCGGATTTTTTTCCCTGGAAGCCATCCCGTCAGAGGTGTGCAAGTGA
  TGAAAGTTGGCAAGCTGCAGCTCCATCAAGGCATGTTTCCTCAAGCCATGAAGAACCTGAGGCTGGCCTT
  TGACATTATGAAAGTGACCCACGGCCGAGAGCACAGCCTGATTGAAGATTTGATTCTTCTCCTGGAAGAA
  TGTGATGCCAACATACGAGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206824 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEALKVEKFTTANRGNGLRAVAPLRPGELLFRSDPLAYTVCKGSRGVVCDRCLLGKEKLMRCSQCRIAKY
  CSAKCQKKAWPDHRRECSCLKSCKPRYPPDSVRLLGRVIVKLMDEKPSESEKLYSFYDLESNISKLTEDK
  KEGLRQLAMTFQHFMREEIQDASQLPPSFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSMSLLNHSCDP
  NCSIVFNGPHLLLRAVREIEAGEELTICYLDMLMTSEERRKQLRDQYCFECDCIRCQTQDKDADMLTGDE
  QIWKEVQESLKKIEELKAHWKWEQVLALCQAIINSNSNRLPDINIYQLKVLDCAMDACINLGMLEEALFY
  AMRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMKVTHGREHSLIEDLILLLEE
  CDANIRAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027188
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027188.1, NM_027188.2, NM_027188.3, NP_081464.1
• RefSeq Size: 3769 bp
• RefSeq ORF: 1287 bp
• Locus ID: 69726
• MW: 49.1 kDa