Eif5 (NM_173363) Mouse Tagged ORF Clone

CAT#: MR206835

Eif5 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 5 (Eif5), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif5
• Synonyms: 2810011H21Rik; D12Ertd549e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206835 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCAACGTCAACCGCAGCGTGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTTTGATTGCTA
  AGGTTGAGGGCAAAGGAAATGGAATCAAGACAGTTATAGTCAACATGGTTGACGTTGCAAAGGCGCTTAA
  TCGGCCTCCAACGTATCCCACCAAATATTTTGGTTGTGAGCTGGGAGCACAGACCCAGTTTGATGTTAAG
  AATGACCGTTACATTGTCAATGGATCTCATGAGGCGAATAAGCTGCAAGACATGTTGGATGGATTCATTA
  AAAAATTTGTTCTCTGTCCTGAGTGTGAGAATCCTGAAACAGATCTGCATGTCAATCCAAAGAAGCAAAC
  AATAGGTAATTCTTGTAAAGCCTGTGGGTACCGAGGCATGCTTGACACACATCATAAACTCTGTACATTC
  ATTCTCAAAAACCCACCTGAGAATAGTGACATTGGTACAGGAAAGAAAGAAAAGGAAAAGAAAAATAGAA
  AGGGCAAGGACAAGGAAAATGGCTCTGTATCTACCAGTGAGACACCACCACCTCCACCACCAAATGAAAT
  TAGTCCTCCACATGCTGTGGAAGAAGAGGAAGATGATGATTGGGGGGAGGATACAACTGAGGAAGCTCAA
  AGGCGCAGAATGGATGAAATCAGTGACCATGCAAAAGGTCTGACACTTAGTGATGATTTGGAAAGAACTG
  TAGAAGAGCGTGTTAACATCCTGTTTGATTTTGTTAAGAAAAAGAAAGAAGAGGGCATTATTGATTCATC
  TGATAAAGAAATTGTGGCTGAGGCAGAAAGACTGGATGTAAAAGCCATGGGTCCTCTTGTTTTGACAGAA
  GTTCTCTTTGATGAGAAGATAAGAGAGCAAATCAAGAAATACAGGCGCCATTTTCTAAGATTTTGTCATA
  ACAACAAAAAGGCTCAGCGGTACCTTCTTCATGGTTTGGAATGTGTGGTAGCAATGCATCAAGCTCAGCT
  GATCTCCAAGATCCCACACATCCTGAAGGAGATGTATGATGCCGACCTGTTAGAGGAGGAGGTCATTATC
  AGCTGGTCAGAAAAGGCCTCTAAGAAATATGTCTCAAAAGAACTTGCCAAAGAGATTCGTGTCAAAGCAG
  AGCCATTTATTAAATGGTTGAAGGAAGCAGAGGAAGAATCTTCTGGTGGTGAGGAAGAAGATGAAGACGA
  AAATATTGAGGTCGTATATTCGAAAACTGCCAGTGTACCAAAAGTTGAAACTGTGAAGTCTGACAACAAG
  GATGATGACATTGATATTGACGCCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206835 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK
  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTF
  ILKNPPENSDIGTGKKEKEKKNRKGKDKENGSVSTSETPPPPPPNEISPPHAVEEEEDDDWGEDTTEEAQ
  RRRMDEISDHAKGLTLSDDLERTVEERVNILFDFVKKKKEEGIIDSSDKEIVAEAERLDVKAMGPLVLTE
  VLFDEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVII
  SWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKTASVPKVETVKSDNK
  DDDIDIDAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173363
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173363.1, NM_173363.2, NM_173363.3, NM_173363.4, NM_173363.5, NP_775539.1
• RefSeq Size: 3966
• RefSeq ORF: 1290
• Locus ID: 217869
• MW: 49 kDa
• Gene Summary: Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). [UniProtKB/Swiss-Prot Function]