Bcl2l13 (NM_153516) Mouse Tagged ORF Clone

CAT#: MR206921

Bcl2l13 (Myc-DDK-tagged) - Mouse BCL2-like 13 (apoptosis facilitator) (Bcl2l13), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcl2l13
• Synonyms: BCL-RAMBO; E430016C20Rik; Mil-1; Mil1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206921 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCTCTACGACTGCGCCTCTAGGATTTCACTATGAAACAAAGTACGTTGTTCTCAGCTACTTGG
  GACTTCTTTCTCAGGAGAAACAACAAGGTCCCTCACCCCCAGGAGTTCAACTAGATGTGGCTCCCCAGTC
  TCTGAATCCAGAAGTTTTATTAAAACTTAAATCTGAGATTGAGGAAGAACTGAAAGCCCTGGACAAAGAA
  GTCTCTGAAGCCTTCACCAGCACAGGCTTTGACTGTCACACCTCGCCTGTGTTTAGCCCTGCTAACCCCG
  AGAGCTCCATAGAAGACTGCTTGGCCCATCTTGGAGAAAGAGTGTCACAAGACCTGAAGGAGCCTTTGCA
  GAAAGCATTGCAGACGATCCTCAGCCAGCCAGTGACATATGAGGCATATCGTGAATGTACAGTGGAGACT
  GCAGTCCATGCCAGCGGATGGAATAAGCTTTTGGTGCCTCTAGTTTTGCTACAACACCTGCTTTTGGAAT
  TGACGCGCCGTGGTCAAGAGCCTTTGCGTATGCTGCTCCAGTTCGGTGTGATGTACCTGGAGGAGCACGC
  CGCAGAGTTCATCATTCAGCAAGGTGGCTGGGGCAGTGTCTTTAGTCTTGAGCCTGAGGAGGAGGAATAC
  CCGGGGATCATTGCGGAAGATAGCAATGACATTTATATACTGCCCAGTGACAACTCCGGACAAGTTAGTC
  CCCCTGAATCCCCGACAGTGACAACATCCTGGCAGTCGGAGAGCCTTCCCGTCTCACTGTCTGCTAGCCA
  GAGCTGGCACACAGAAAGCCTCCCGGTGTCGCTCGGGCCCGAGTCCTGGCAGCAGATTGCAATGGACCCT
  GAAGAAGTGAAGAGCTTAGACAGCAGCGGGGCTGGGGAGAAGAGTGAGAACAACTCGTCCAACTCTGACA
  TCGTGCACGTGGAGAAGGAGGAGGTGCCAGAGGAGGCCTTCCCGGGAGCGGCTGCTCCCTTGCTCGCACA
  GGTCCCTACCGTGGAGGCCCCAGAAATGATGAGAGCTGAGAAAACGAGCCCCACTCCGTCTGTGTTTGTG
  GAGCTTGGTGAGGAGGAGCTGGAAGCAGTAACAGCGAGACCCGAGGCTGTCGAGAGGGCGGAGGGGGCCG
  CTCAGCTGAGTGAGGAGAGAGCCGGGAGCAGGAAAAAGAGCCACACTGGGGAGGCCGCCGCTGTCAGGGG
  GGCAAAGAGTGGCCTGCCCGCCGAGGGCAAGGCCGTACTGCTCTTTGGTGGGGCTGCTGCGGTGGCCATC
  CTGGCAGTGGCTGTTGGAGTTGCACTGGCTTTAAGAAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206921 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSTTAPLGFHYETKYVVLSYLGLLSQEKQQGPSPPGVQLDVAPQSLNPEVLLKLKSEIEEELKALDKE
  VSEAFTSTGFDCHTSPVFSPANPESSIEDCLAHLGERVSQDLKEPLQKALQTILSQPVTYEAYRECTVET
  AVHASGWNKLLVPLVLLQHLLLELTRRGQEPLRMLLQFGVMYLEEHAAEFIIQQGGWGSVFSLEPEEEEY
  PGIIAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMDP
  EEVKSLDSSGAGEKSENNSSNSDIVHVEKEEVPEEAFPGAAAPLLAQVPTVEAPEMMRAEKTSPTPSVFV
  ELGEEELEAVTARPEAVERAEGAAQLSEERAGSRKKSHTGEAAAVRGAKSGLPAEGKAVLLFGGAAAVAI
  LAVAVGVALALRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153516
• ORF Size: 1305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153516.1, NM_153516.2, NP_705736.1
• RefSeq Size: 6933 bp
• RefSeq ORF: 1305 bp
• Locus ID: 94044
• MW: 46.7 kDa
• Gene Summary: May promote the activation of caspase-3 and apoptosis. [UniProtKB/Swiss-Prot Function]