Gcdh (NM_001044744) Mouse Tagged ORF Clone

CAT#: MR206984

Gcdh (Myc-DDK-tagged) - Mouse glutaryl-Coenzyme A dehydrogenase (Gcdh), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gcdh
• Synonyms: 9030411L18; AI266902; D17825; GCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206984 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGAGAGGAGTCTCCGCGCGGTTGCTGAGCCGCAGGTCCGGCCTGCGCTTCCCGCGTTTTCCAC
  GTACCTGGAGCTCGGCGGCCGCCCACACCGAGAAGACACAGATCCGACCGGCCAAATCCTCTCGCCCCGT
  GTTTGACTGGAAGGACCCACTCATACTGGAGGAGCAGCTGACTGCCGATGAGAAACTGATCAGGGACACC
  TTCCGTAACTACTGCCAGGAGCGGCTTATGTCTCGAATTCTGCTGGCTAATCGAAATGAAGTTTTTCACA
  GGGACATTGTGTATGAGATGGGGGAGCTGGGCGTGTTGGGACCCACCATTAAAGGGTATGGCTGTGCTGG
  TGTGTCGTCGGTGGCCTATGGGCTCCTGACCCGAGAGCTTGAGAGGGTGGACAGTGGCTACAGGTCGATG
  ATGAGTGTTCAGTCCTCCCTTGTCATGCACCCCATCTATACCTATGGGAGCGAGGAACAGCGACAGAAAT
  ATCTGCCCGGACTGGCCAAGGGTGAACTTCTGGGCTGCTTTGGACTTACAGAGCCCAACCATGGGAGTGA
  CCCAGGTGGCATGGAGACCAGAGCTCGCCACAATCCATCAAACCAGAGCTACACTCTCAGTGGGACCAAG
  ACCTGGATCACCAACTCCCCTGTGGCTGACCTATTTATAGTGTGGGCTCGGTGTGAGGATAACTGTATTC
  GGGGCTTCATACTGGAGAAGGGTATGCGGGGCCTCTCAGCCCCTAGGATTGAAGGAAAGTTCTCCTTGCG
  GGCCTCGGCTACCGGTATGATCATCATGGACAGTGTGGAAGTGCCTGAGGAGAATGTGCTGCCTAATGTA
  TCCAGCCTGGCGGGCCCTTTTGGCTGCCTTAACACTGCCCGCTATGGCATCACATGGGGTGTGTTGGGAG
  CTGCTGAGTTCTGTTTGCACACAGCCCGGCAGTATGCCCTAGACAGGATCCAGTTTGGAGTCCCCTTGGC
  CAGGAACCAGCTGGTTCAGAAGAAGTTGGCAGACATGCTCACTGAGATCACACTGGGACTCCATGCTTGT
  TTGCAACTTGGCCGTTTAAAGGATCAGGACAAGGCTACCCCAGAGATGGTCTCCATGTTGAAGAGAAACA
  ACTGTGGAAAAGCCCTGGATATTGCCCGCCAGGCACGAGACATCCTGGGAGGAAATGGGATTTCTGATGA
  GTACCATGTCATCCGGCATGCTATGAATCTGGAGGCAGTGAACACTTATGAAGGTACACATGACATTCAT
  GCTCTGATCCTTGGGAGAGCAATTACTGGGATTCAGGCATTCACGGTTGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206984 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLRGVSARLLSRRSGLRFPRFPRTWSSAAAHTEKTQIRPAKSSRPVFDWKDPLILEEQLTADEKLIRDT
  FRNYCQERLMSRILLANRNEVFHRDIVYEMGELGVLGPTIKGYGCAGVSSVAYGLLTRELERVDSGYRSM
  MSVQSSLVMHPIYTYGSEEQRQKYLPGLAKGELLGCFGLTEPNHGSDPGGMETRARHNPSNQSYTLSGTK
  TWITNSPVADLFIVWARCEDNCIRGFILEKGMRGLSAPRIEGKFSLRASATGMIIMDSVEVPEENVLPNV
  SSLAGPFGCLNTARYGITWGVLGAAEFCLHTARQYALDRIQFGVPLARNQLVQKKLADMLTEITLGLHAC
  LQLGRLKDQDKATPEMVSMLKRNNCGKALDIARQARDILGGNGISDEYHVIRHAMNLEAVNTYEGTHDIH
  ALILGRAITGIQAFTVGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001044744
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001044744.1, NP_001038209.2
• RefSeq Size: 2140
• RefSeq ORF: 1344
• Locus ID: 270076
• MW: 48.6 kDa
• Gene Summary: Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. [UniProtKB/Swiss-Prot Function]