E2f2 (NM_177733) Mouse Tagged ORF Clone

CAT#: MR207069

E2f2 (Myc-DDK-tagged) - Mouse E2F transcription factor 2 (E2f2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2f2
• Synonyms: E2F-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207069 representing NM_177733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCGCGCCGCGGACCCTGGCTCCGGCCACGGCGCAACCTACAAAGAGCTTGCCGGCGCTGAACC
  CCACCGAGCTGTGGCCTTCGGGTCTGAGCAGCCCCCAGCTCTGCCCGGCCACCACCGCCACCACCTACTA
  CACTTCGCTTTACACGCAGACGGTGCCTTCCTCTGTGGCGCTGGGCACCTGCCTCGACGCCACTCCCCAC
  GGACCCGAGGGCCAAATTGTGCGATGTGCACCCGCAGGCCGGCTGCCGGCCAAAAGGAAGTTGGACCTGG
  AGGGCATTGGGAGGCCTACGGTCCCTGAATTCCGGACCCCCAAGGGGAAGTGCATCCGCGTGGATGGTTT
  GCCAAGCCCCAAAACCCCCAAGTCTCCTGGGGAGAAGACACGCTATGACACGTCGCTGGGGCTCCTGACC
  AAGAAGTTCATTTACCTCCTGAGCGAGTCGGAGGATGGAGTCCTGGACCTGAACTGGGCAGCCGAGGTGC
  TGGATGTGCAAAAGCGGCGCATCTATGACATCACCAACGTGCTGGAGGGTATCCAGCTCATCCGCAAGAA
  GTCCAAAAACAACATCCAGTGGGTAGGCAGGGAACTATTTGAAGACCCCACCCGACCCTCCAGGCAGCAG
  CAGTTGGGGCAGGAGCTGAAGGAGCTGATGAATGCCGAGCAGACCTTGGACCAGCTCATTCAGAGTTGCT
  CCCTGAGCTTCAAGCACCTGACCGAAGATAATGCCAACAAGAAACTGGCCTATGTGACCTACCAGGATAT
  CCGTGCCGTAGGCAACTTCAAGGAGCAGACAGTGATTGCGGTCAAGGCCCCACCACAGACAAGATTGGAA
  GTGCCGGACAGGGCCGAGGAGAACCTGCAGATTTATCTAAAGAGTACCCAAGGCCCCATTGAAGTCTACC
  TGTGCCCAGAGGAGGGGCAGGAGCCAGACAGTCCTGCCAAGGAGGCGCTCCCCTCCACCTCTGCCCTCAG
  CCCCATTCCTGACTGCGCTCAGCCGGGCTGCAGCACTGACTCTGGGATCGCAGAGACCATAGAGCCTTCA
  GTACTGATACCCCAGCCGATACCACCGCCTCCTCCACCACCACTGCCGCCAGCCCCATCCCTCGTCCCCT
  TGGAAGCCACTGACAACATGCTGGAGCTGTCACACCCTCTTCTACAACAGACTGAGGACCAGTTCCTGTC
  CCCAATCCTGGCGGCCAACTCCCCCCTGATCAGCTTCTCCCCGCCCTTGGACCAGGACGAATACCTGTGG
  GGCATGGACGAGGGGGAAGGCATCAGTGACCTCTTCGACTCCTATGACCTTGGGGACCTGTTGATTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207069 representing NM_177733
  Red=Cloning site Green=Tags(s)
  
  MLRAPRTLAPATAQPTKSLPALNPTELWPSGLSSPQLCPATTATTYYTSLYTQTVPSSVALGTCLDATPH
  GPEGQIVRCAPAGRLPAKRKLDLEGIGRPTVPEFRTPKGKCIRVDGLPSPKTPKSPGEKTRYDTSLGLLT
  KKFIYLLSESEDGVLDLNWAAEVLDVQKRRIYDITNVLEGIQLIRKKSKNNIQWVGRELFEDPTRPSRQQ
  QLGQELKELMNAEQTLDQLIQSCSLSFKHLTEDNANKKLAYVTYQDIRAVGNFKEQTVIAVKAPPQTRLE
  VPDRAEENLQIYLKSTQGPIEVYLCPEEGQEPDSPAKEALPSTSALSPIPDCAQPGCSTDSGIAETIEPS
  VLIPQPIPPPPPPPLPPAPSLVPLEATDNMLELSHPLLQQTEDQFLSPILAANSPLISFSPPLDQDEYLW
  GMDEGEGISDLFDSYDLGDLLIN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177733
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177733.1, NM_177733.2, NM_177733.3, NM_177733.4, NM_177733.5, NM_177733.6, NM_177733.7, NP_808401.1
• RefSeq Size: 4866
• RefSeq ORF: 1332
• Locus ID: 242705
• MW: 48.5 kDa
• Gene Summary: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. [UniProtKB/Swiss-Prot Function]