Tubb4a (NM_009451) Mouse Tagged ORF Clone

CAT#: MR207086

Tubb4a (Myc-DDK-tagged) - Mouse tubulin, beta 4 (Tubb4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tubb4a
• Synonyms: AI325297; M(beta)4; Tubb; Tubb4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207086 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGAAATCGTGCACCTGCAAGCCGGTCAATGCGGTAACCAGATCGGGGCCAAGTTCTGGGAGGTCA
  TCAGTGATGAGCACGGCATCGACCCCACTGGGACCTATCATGGGGACAGTGACCTCCAGCTGGAGAGGAT
  CAATGTCTACTACAATGAGGCCACAGGTGGAAACTATGTTCCCAGAGCCGTGCTGGTCGACCTGGAACCC
  GGCACCATGGACTCTGTCCGCTCCGGCCCTTTTGGCCAGATCTTTCGGCCAGACAACTTTGTATTTGGTC
  AATCCGGAGCAGGCAACAACTGGGCCAAGGGTCACTACACCGAGGGCGCGGAGTTAGTGGATGCCGTCCT
  GGACGTGGTGCGCAAAGAGGCGGAAAGCTGCGACTGTCTCCAGGGCTTCCAGCTCACCCACTCGCTCGGA
  GGTGGCACCGGCTCAGGCATGGGGACCTTGCTCATCAGCAAGATCCGAGAGGAGTTTCCAGACAGGATCA
  TGAATACGTTCAGCGTGGTGCCATCACCCAAGGTGTCTGACACGGTGGTGGAGCCCTACAATGCCACACT
  GTCTGTGCATCAGCTGGTGGAGAACACTGATGAGACCTACTGCATCGACAACGAGGCCTTGTACGACATC
  TGCTTCCGCACGCTCAAGCTGACCACGCCCACCTACGGGGACCTCAACCACCTCGTGTCAGCCACCATGA
  GTGGAGTCACCACCTGCCTACGTTTCCCGGGCCAGCTCAATGCAGATCTACGCAAGCTGGCTGTGAATAT
  GGTGCCATTCCCCCGTCTCCACTTCTTCATGCCAGGATTCGCACCCTTGACCAGCAGGGGCAGCCAGCAG
  TACCGGGCCCTCACCGTTCCTGAGCTGACCCAACAGATGTTCGATGCTAAGAACATGATGGCTGCGTGTG
  ACCCGAGACACGGTCGCTACCTGACTGTGGCTGCTGTCTTCCGGGGACGGATGTCCATGAAGGAGGTAGA
  CGAGCAGATGTTAAGTGTGCAGAGCAAGAACAGCAGTTACTTCGTTGAGTGGATCCCCAACAATGTCAAG
  ACAGCCGTATGTGACATCCCACCCCGGGGCCTGAAGATGGCAGCCACCTTCATCGGCAACAGCACTGCCA
  TCCAGGAGCTGTTCAAGCGCATCTCGGAGCAGTTCACCGCCATGTTCAGACGCAAGGCCTTCCTGCACTG
  GTACACGGGCGAAGGCATGGACGAGATGGAGTTTACGGAAGCAGAGAGCAATATGAACGACCTGGTGTCC
  GAGTACCAGCAGTACCAGGATGCCACTGCTGAAGAGGGCGAGTTCGAAGAGGAGGCTGAAGAGGAGGTGG
  CT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207086 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEP
  GTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLG
  GGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDI
  CFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQ
  YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVK
  TAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
  EYQQYQDATAEEGEFEEEAEEEVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009451
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009451.1, NM_009451.2, NM_009451.3, NP_033477.2
• RefSeq Size: 2246 bp
• RefSeq ORF: 1335 bp
• Locus ID: 22153
• MW: 49.6 kDa
• Gene Summary: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProtKB/Swiss-Prot Function]