Noxa1 (NM_172204) Mouse Tagged ORF Clone

CAT#: MR207092

Noxa1 (Myc-DDK-tagged) - Mouse NADPH oxidase activator 1 (Noxa1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Noxa1
• Synonyms: NY-CO-31; SDCCAG31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207092 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCTCTAGGGGATCAGATACGGGACTGGCACCGGGGTGTGCTGGCCGTGGCACGCGAAGACTGGG
  ACTCTGCGCTGTGCTTCTTCTCAGATGTCCGAGAGCCGCTGGCTAGGATGTACTTTAACAGGGGCTGTGT
  GCATCTGATGGCAGGGGATCCCGAGGCTGCGCTGCGGGCATTTGACCAAGCAGTGACTAAGGACACCTGC
  ATGGCTGTTGGCTTCCTCCAGCGGGGAGTGGCCAATTTCCAGCTGCAGAGGTTCCAGGAGGCTGTGTCTG
  ACTTCCAGTTGGCCCTGGCACAGCTGAGGGACAATGCTGTCATTGACTACACACAACTGGGTCTGAACTT
  CAAATTGCAAGCCTGGGAGGTCCTATACAACATGGCATCAGCACAGTGCCAGGCAGGGCTCTGGACCAAG
  GCTGCCAATACTCTAGTGGAGGCAATCTCCAAATGGCCAGAGGGGGCTCAAGACATCCTGGACATTGCCA
  TGGACAAAGTGCAGAAACAGGTACCCCTACAGCTACAGCAAGTGCCCAAGGGTGAGGTCTTCCAGCCTCC
  CAGGCGATACCTAAAACATCTGGAGCCCATGGATTTCCTTGGCAAGGCTAAGGTGGTGGCTTCTGTCATT
  CCTGATGACCACAACGCCCAGCCTCAGCAGAGGTCCCAGGCGGAGCATGCTGGCCACCAGCCATCCTCAT
  CTACGTGTAAGAGGGTCCTGAGCACTACGGGTGGTCACACGAGCCCTGGCCTATATGATAGTTTGCTGGC
  ATCTGGAAGGCCTGGTCCAGGCCCCTCTGAAGTTTCCTCAGGATCTGAGGGAGCAGCTACAAAGGACCCT
  GAATCCTTGGTGACTGTCACTGTGCAGTGCCACTTTACTGTGCCCCTGAAGGTCCCAAGAGGAACTGGCC
  TGTCCAGTTTTCAGACACTACTAGCTCAAGCCCTCCTTCACCAGACGCAGACAGGGCAGCTCAGTTACAA
  AGCCCCAGGAGAGGAGAGATCCTGGATTCCCATCTCCACGGAGGAGTCCCTGCAGAGTATATGGAGGAAT
  GTGCCCGTGGGCCCAGGAGGGTTGCAGCTCCAGTGCCAGGGGGTCTGGGGCCGGCCAGTCCTCTACCAAG
  TAGTAGCTCAGTATAACTATCGTGCCCAAAGACCGGAGGATTTGGACTTCCACCAAGGGGACACGGTGGA
  TGTTCTGTGTGAAGTGGACGAAGCATGGCTGGAGGGACACCGAGATGGCTGCGTTGGCATTTTCCCTAAG
  TGCTTTGTGGTCCCAGCTGGCGCCTATGTGGAAGCCATGCTTGTACTGGGACCCCAGCCAGGAGACCAGA
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207092 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSLGDQIRDWHRGVLAVAREDWDSALCFFSDVREPLARMYFNRGCVHLMAGDPEAALRAFDQAVTKDTC
  MAVGFLQRGVANFQLQRFQEAVSDFQLALAQLRDNAVIDYTQLGLNFKLQAWEVLYNMASAQCQAGLWTK
  AANTLVEAISKWPEGAQDILDIAMDKVQKQVPLQLQQVPKGEVFQPPRRYLKHLEPMDFLGKAKVVASVI
  PDDHNAQPQQRSQAEHAGHQPSSSTCKRVLSTTGGHTSPGLYDSLLASGRPGPGPSEVSSGSEGAATKDP
  ESLVTVTVQCHFTVPLKVPRGTGLSSFQTLLAQALLHQTQTGQLSYKAPGEERSWIPISTEESLQSIWRN
  VPVGPGGLQLQCQGVWGRPVLYQVVAQYNYRAQRPEDLDFHQGDTVDVLCEVDEAWLEGHRDGCVGIFPK
  CFVVPAGAYVEAMLVLGPQPGDQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172204
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172204.1, NM_172204.2, NM_172204.3, NM_172204.4, NP_757341.2
• RefSeq Size: 1673 bp
• RefSeq ORF: 1335 bp
• Locus ID: 241275
• MW: 48.8 kDa