Rarb (NM_011243) Mouse Tagged ORF Clone

CAT#: MR207145

Rarb (Myc-DDK-tagged) - Mouse retinoic acid receptor, beta (Rarb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rarb
• Synonyms: A830025K23; Hap; Nr1b2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207145 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGACTGTATGGATGTTCTGTCAGTGAGTCCCGGGCAGATCCTGGATTTCTACACCGCGAGCCCTT
  CCTCCTGCATGCTGCAGGAAAAGGCTCTCAAAGCCTGCCTCAGTGGATTCACCCAGGCCGAATGGCAGCA
  CCGGCATACTGCTCAATCCATCGAGACACAGAGTACCAGCTCTGAGGAGCTCGTCCCGAGCCCACCATCT
  CCACTTCCTCCTCCTCGGGTGTACAAGCCCTGCTTCGTTTGCCAGGACAAGTCATCGGGCTACCACTATG
  GCGTCAGTGCCTGCGAGGGGTGCAAGGGCTTTTTCCGCAGAAGTATTCAGAAGAACATGATCTACACTTG
  CCATCGAGATAAGAACTGCGTCATTAACAAGGTCACTAGGAACCGATGCCAGTACTGCCGCCTGCAGAAG
  TGCTTTGAAGTGGGCATGTCCAAAGAGTCTGTTAGGAATGACAGGAACAAGAAAAAGAAGGAGCCTTCAA
  AGCAGGAATGCACAGAGAGCTATGAGATGACAGCGGAGCTAGACGACCTCACTGAGAAGATCCGGAAAGC
  CCACCAGGAAACCTTTCCCTCACTCTGCCAGCTGGGTAAATACACCACGAATTCCAGCGCTGACCACCGG
  GTCCGATTGGACTTGGGCCTCTGGGACAAATTCAGTGAGCTGGCCACCAAGTGCATTATTAAGATCGTGG
  AGTTCGCCAAGCGTCTGCCGGGCTTCACAGGTCTGACCATCGCAGACCAGATCACCCTGCTCAAAGCCGC
  CTGCTTGGATATCTTGATTCTCAGAATTTGTACCAGGTATACCCCAGAGCAAGACACCATGACTTTCTCT
  GATGGCCTTACACTAAATCGAACTCAGATGCACAATGCTGGCTTCGGTCCTCTGACTGACCTTGTGTTCA
  CCTTTGCCAACCAGCTCCTGCCTTTGGAAATGGATGACACAGAAACAGGCCTTCTCAGTGCCATCTGTTT
  AATCTGTGGAGACCGCCAGGACCTTGAGGAACCAACAAAAGTAGACAAGCTCCAAGAACCACTGCTGGAA
  GCACTAAAGATTTACATTAGAAAACGACGACCCAGCAAGCCTCACATGTTTCCAAAGATCTTAATGAAAA
  TCACAGATCTCCGCAGCATCAGCGCGAAAGGTGCCGAACGTGTAATTACCTTGAAAATGGAAATTCCTGG
  ATCAATGCCACCTCTCATTCAGGAAATGCTGGAGAATTCTGAAGGACATGAACCCTTGACCCCAAGTTCA
  AGTGGGAATATAGCAGAGCACAGTCCCAGCGTGTCCCCCAGCTCAGTGGAGAACAGTGGAGTCAGTCAGT
  CACCACTGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207145 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFDCMDVLSVSPGQILDFYTASPSSCMLQEKALKACLSGFTQAEWQHRHTAQSIETQSTSSEELVPSPPS
  PLPPPRVYKPCFVCQDKSSGYHYGVSACEGCKGFFRRSIQKNMIYTCHRDKNCVINKVTRNRCQYCRLQK
  CFEVGMSKESVRNDRNKKKKEPSKQECTESYEMTAELDDLTEKIRKAHQETFPSLCQLGKYTTNSSADHR
  VRLDLGLWDKFSELATKCIIKIVEFAKRLPGFTGLTIADQITLLKAACLDILILRICTRYTPEQDTMTFS
  DGLTLNRTQMHNAGFGPLTDLVFTFANQLLPLEMDDTETGLLSAICLICGDRQDLEEPTKVDKLQEPLLE
  ALKIYIRKRRPSKPHMFPKILMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGHEPLTPSS
  SGNIAEHSPSVSPSSVENSGVSQSPLLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011243
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011243.1, NM_011243.2, NP_035373.1
• RefSeq Size: 3041 bp
• RefSeq ORF: 1347 bp
• Locus ID: 218772
• MW: 50.3 kDa