Pfkfb4 (BC057594) Mouse Tagged ORF Clone

CAT#: MR207244

Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb4
• Synonyms: C230090D14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207244 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCCACGGGAACTGACCCAGAATCCCCTGAAGAAGATCTGGATGCCGTACAGCAATGGCAGGC
  CCGCTCTGCACGCCAGCCAGCGTGGTGCCCGGGGCAAGACCTACATTTCTAAGAAGCTGACGCGGTACCT
  CAACTGGATTGGCGTGCCTACTCGGGAATTCAACGTTGGTCAGTATCGCCGGGACATAGTCAAGACGTAC
  AAATCTTTTGAGTTTTTCCTCCCAGACAATGAAGAAGGCCTGAAAATCAGGAAGCAGTGTGCCTTAGCAG
  CCCTCAGTGATGTCCGGAAGTTCCTCAGTGAGGAGGGGGGACATGTGGCGGTTTTTGATGCAACCAATAC
  GACCCGAGAGCGGAGAGCGATGATCTTTAACTTTGGAGAACAGAATGGCTACAAGACCTTCTTTGTTGAA
  TCTATCTGTGTGGATCCTGAGGTTGTTGCTGCCAACATCGTGCAAGTGAAGCTGGGCAGCCCTGACTATG
  TCAACCGGGACAGCGACGAAGCCACCGAAGACTTCATGAGGCGCATTGAATGCTATGAAAACTCATATGA
  GTCGCTGGATGAAGACCTGGACAGGGATCTGTCCTACATCAAGATCATGGACGTGGGCCAGAGCTATGTG
  GTGAACCGTGTTGCTGATCACATCCAGAGTCGCATCGTTTATTACCTCATGAACATCCATGTGACACCCC
  GCTCCATCTACCTCTGCCGGCATGGGGAGAGCGAGCTAAACCTCAAGGGCCGGATTGGTGGGGATCCTGG
  ACTGTCCCCCCGGGGCCGGGAGTTTTCCAAGCATCTGGCCCAGTTCATCAGTGACCAGAACATTAAGGAT
  CTGAAGGTCTGGACGAGCCAGATGAAGAGGACGATCCAGACAGCCGAGGCGCTGAGCGTCCCTTATGAGC
  AGTGGAAGGTCCTCAACGAGATCGATGCGGGCGTCTGTGAGGAAATGACCTACGAAGAAATCCAGGATCA
  CTACCCGCTGGAGTTTGCCCTGCGGGATCAGGACAAGTACCGGTACCGGTACCCGAAGGGTGAGTCCTAT
  GAAGACCTGGTGCAGCGGCTGGAGCCCGTCATCATGGAACTGGAGAGGCAGGAGAATGTGTTGGTCATTT
  GCCACCAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCCTTGACAAGGCAGCTGAAGAGCTGCCCTACCT
  CAAATGCCCCTTGCACACAGTCCTGAAGCTCACACCCGTGGCTTACGGTTGTAAAGTGGAGTCCATATTC
  CTGAACGTGGCAGCTGTGAACACTCACCGAGACAGACCTCAGAATGTAGACATATCCAGGCCTTCAGAGG
  AAGCCCTTGTCACAGTCCCTGCTCACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207244 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPRELTQNPLKKIWMPYSNGRPALHASQRGARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDIVKTY
  KSFEFFLPDNEEGLKIRKQCALAALSDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVE
  SICVDPEVVAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYV
  VNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKD
  LKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESY
  EDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIF
  LNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057594
• ORF Size: 1359 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC057594, AAH57594
• RefSeq Size: 3329
• RefSeq ORF: 1361
• Locus ID: 270198
• MW: 52.4 kDa