Ephx1 (NM_010145) Mouse Tagged ORF Clone

CAT#: MR207267

Ephx1 (Myc-DDK-tagged) - Mouse epoxide hydrolase 1, microsomal (Ephx1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ephx1
• Synonyms: EH; Eph-1; Eph1; Epxh1; mEH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207267 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGAACTCATCCTGGCTTCTGTGCTGGGCTTTGTCATCTACTGGTTTGTCTCCCGGGACAAGG
  AGGAGACCTTACCACTTGAAGATGGGTGGTGGGGCCCAGGGTCAAAGCCATCAGCCAAAGAAGATGAGAG
  CATCCGGCCCTTCAAGGTGGAAACATCAGATGAGGAGATCAAGGACTTGCACCAGAGGATAGATAGGTTC
  CGGGCATCCCCACCTTTGGAGGGCAGTCGCTTCCACTATGGCTTCAACTCCAGCTACCTGAAGAAAGTGG
  TGTCCTTCTGGAGGAATGAGTTTGACTGGAGGAAGCAGGTGGAGATCCTCAACCAATACCCACACTTTAA
  GACCAAGATTGAAGGGCTGGACATCCACTTCATCCACGTGAAACCTCCCCAGCTGCCCTCAGGCCGCACT
  CCAAAGCCCTTGCTGATGGTGCACGGCTGGCCTGGCTCCTTCTATGAGTTCTACAAGATTATCCCACTGC
  TGACAGACCCCAAGACCCACGGCCTGAGTGATGAGCACGTGTTTGAAGTCATCTGTCCCTCAATTCCTGG
  CTATGGCTTCTCAGAGGCATCCAGCAAGAAAGGTTTAAATTCGGTGGCCACTGCGAGGATCTTCTACAAG
  CTGATGTCACGGCTGGGCTTCCAGAAGTTCTACATTCAAGGCGGCGACTGGGGGTCTCTCATCTGCACCA
  ACATAGCCCAGATGGTGCCCAACCACGTGAAAGGTTTGCACTTGAATATGTCTTTCATTTCAAGAAACAT
  TTATTCCCTGACCCCTCTCCTGGGCCAACGTTTTGGGAGATTTCTTGGCTACACAGAGAAGGATCTGGAG
  CTCTTGTACCCATTCAAGGAAAAGGTTTTCTACAACATCATGAGGGAGAGTGGCTACTTACACATCCAGG
  CCACCAAGCCGGACACTGTGGGCTGTGCTCTGAATGACTCTCCTGTGGGCCTGGCTGCCTACATCTTAGA
  GAAGTTCTCCACCTGGACCAAGTCAGAATACCGTGAACTGGAGGATGGAGGCCTGGAGAGGAAGTTCTCC
  CTGGAAGATCTGCTGACTAACATCATGATCTACTGGACGACAGGAACCATTGTCTCCTCCCAGCGCTTCT
  ACAAGGAAAACTTGGGCCAGGGTGTCATGGTCCATAGACATGAGGGGATGAAGGTCTTTGTGCCCACTGG
  CTATTCAGCCTTCCCTTCTGAGATCCTGCATGCCCCAGAAAAGTGGGTGAAGGTCAAGTACCCCAAACTC
  ATCTCCTATTCCTACATGGAACGTGGGGGCCACTTTGCTGCCTTCGAAGAGCCCAAGCTTCTGGCCCAGG
  ACATCCGCAAGTTCGTGTCCCTGGCTGAGCTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207267 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWLELILASVLGFVIYWFVSRDKEETLPLEDGWWGPGSKPSAKEDESIRPFKVETSDEEIKDLHQRIDRF
  RASPPLEGSRFHYGFNSSYLKKVVSFWRNEFDWRKQVEILNQYPHFKTKIEGLDIHFIHVKPPQLPSGRT
  PKPLLMVHGWPGSFYEFYKIIPLLTDPKTHGLSDEHVFEVICPSIPGYGFSEASSKKGLNSVATARIFYK
  LMSRLGFQKFYIQGGDWGSLICTNIAQMVPNHVKGLHLNMSFISRNIYSLTPLLGQRFGRFLGYTEKDLE
  LLYPFKEKVFYNIMRESGYLHIQATKPDTVGCALNDSPVGLAAYILEKFSTWTKSEYRELEDGGLERKFS
  LEDLLTNIMIYWTTGTIVSSQRFYKENLGQGVMVHRHEGMKVFVPTGYSAFPSEILHAPEKWVKVKYPKL
  ISYSYMERGGHFAAFEEPKLLAQDIRKFVSLAELQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010145
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010145.1, NM_010145.2, NM_010145.3, NP_034275.1
• RefSeq Size: 1708 bp
• RefSeq ORF: 1368 bp
• Locus ID: 13849
• MW: 52.6 kDa
• Gene Summary: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. [UniProtKB/Swiss-Prot Function]