Psmd12 (NM_025894) Mouse Tagged ORF Clone

CAT#: MR207290

Psmd12 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (Psmd12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psmd12
• Synonyms: 1500002F15Rik; AI480719; P55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207290 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGTGGCTCGGAGCGGGCCGATGGGCGCATTGTGAAGATGGAAGTGGACTACAGCGCCACGG
  TGGACCAGCGCCTGCCTGAGTGCGAGAAGCTGGCCAAGGAAGGGCGGCTTCAAGAAGTCATCGAAACCCT
  TCTCTCTCTAGAAAAGCAGACCCGGACTGCCTCTGACATGGTGTCCACGTCCCGCATCTTGGTTGCAGTA
  GTGAAAATGTGCTATGAGGCTAAAGAATGGGACTTACTTAATGAAAATATTATGCTTCTGTCAAAACGAC
  GGAGTCAGTTAAAACAAGCAGTTGCAAAAATGGTTCAGCAGTGCTGTACTTACGTGGAGGAGATCACAGA
  CCTGCCAGTCAAGCTGCGATTGATTGATACTCTGCGGATGGTTACAGAAGGAAAGATTTATGTTGAAATT
  GAGCGTGCTCGGCTGACTAAAACCTTAGCAACTATTAAAGAGCAAAATGGCGATGTGAAAGAGGCTGCCT
  CCATCCTTCAAGAGTTACAGGTGGAAACCTATGGGTCCATGGAGAAGAAGGAGCGAGTGGAATTCATTCT
  GGAGCAGATGAGACTCTGCCTAGCTGTGAAGGATTACATTCGCACACAGATCATTAGCAAAAAAATTAAT
  ACCAAATTCTTCCAAGAAGAAAACACAGAGAACTTGAAGTTGAAGTACTATAACTTAATGATTCAGCTGG
  ATCAGCACGAGGGGTCCTATCTGTCCATCTGCAAACACTACAGAGCCATCTATGACACTCCCTGCATTCA
  GGCAGAAAGTGACAAGTGGCAGCAGGCTCTGAAAAGTGTTGTTCTCTATGTTATCCTGGCTCCTTTTGAC
  AATGAGCAATCAGATTTGGTTCACCGGATAAGCAGTGACAAGAAGCTGGAAGAAATTCCCAAATACAAGG
  ATCTGCTGAAGCTTTTTACCACCATGGAGCTGATGCGCTGGTCCACTCTTGTTGAGGACTATGGGGTGGA
  GCTGCGAAAAGGCTCCTCAGAGACCCCGGCGACTGACGTTTTCAGCTCTACAGAAGAAGGGGAAAAGAGG
  TGGAAAGACTTGAAGAGCAGAGTGGTGGAGCACAACATCAGAATTATGGCCAAGTACTACACTCGGATAA
  CAATGAAAAGGATGGCACAACTTCTGGATCTCTCTGTGGATGAGTCAGAGGCTTTCCTCTCGAATCTAGT
  CGTTAACAAGACCATCTTTGCTAAAGTAGACAGGTTGGCTGGAGTCATCAACTTCCAGAGACCCAAGGAT
  CCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCGCTGATGTCTCTGGTTAACAAAACGACAC
  ACCTCATAGCCAAAGAGGAGATGATACATAATCTACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207290 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADGGSERADGRIVKMEVDYSATVDQRLPECEKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAV
  VKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPVKLRLIDTLRMVTEGKIYVEI
  ERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKIN
  TKFFQEENTENLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESDKWQQALKSVVLYVILAPFD
  NEQSDLVHRISSDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGVELRKGSSETPATDVFSSTEEGEKR
  WKDLKSRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGVINFQRPKD
  PNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025894
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025894.1, NM_025894.2, NP_080170.1
• RefSeq Size: 1723 bp
• RefSeq ORF: 1371 bp
• Locus ID: 66997
• MW: 52.9 kDa
• Gene Summary: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. [UniProtKB/Swiss-Prot Function]