Irak4 (NM_029926) Mouse Tagged ORF Clone

CAT#: MR207322

Irak4 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 4 (Irak4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Irak4
• Synonyms: 8430405M07Rik; 9330209D03Rik; IRAK-4; NY-REN-64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207322 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGCCGTTGACACCATCGACATACATACGCAACCTTAATGTGGGGATCCTTAGGAAGCTGTCGG
  ATTTTATTGATCCTCAAGAAGGGTGGAAGAAATTAGCAGTAGCTATCAAAAAGCCGTCCGGCGACGACAG
  ATACAATCAGTTCCATATAAGGAGATTCGAAGCCTTACTTCAGACCGGGAAGAGCCCCACCTGTGAACTG
  CTGTTTGACTGGGGCACCACGAACTGCACAGTTGGCGACCTTGTGGATCTACTGGTCCAGATTGAGCTGT
  TTGCCCCCGCCACTCTCCTGCTGCCGGATGCCGTTCCCCAAACCGTCAAAAGCCTGCCTCCTAGAGAAGC
  GGCAACAGTGGCACAAACACACGGGCCTTGTCAGGAAAAGGACAGGACATCCGTAATGCCTATGCCGAAG
  CTAGAACACAGCTGCGAGCCACCGGACTCCTCAAGCCCAGACAACAGAAGTGTAGAGTCCAGCGACACTC
  GGTTCCACAGCTTCTCGTTCCATGAACTGAAGAGCATCACAAACAACTTCGACGAGCAACCCGCGTCTGC
  CGGTGGCAACCGGATGGGAGAGGGGGGATTTGGAGTGGTGTACAAGGGCTGTGTGAACAACACCATCGTG
  GCGGTGAAGAAGCTCGGAGCGATGGTTGAAATCAGTACTGAAGAACTAAAGCAACAGTTTGATCAAGAAA
  TTAAAGTAATGGCAACGTGTCAGCACGAGAACCTGGTGGAGCTGCTCGGCTTCTCCAGCGACAGCGACAA
  CCTGTGCTTAGTGTATGCTTACATGCCCAACGGGTCCTTGCTGGACAGACTGTCCTGCCTGGATGGTACA
  CCACCGCTTTCCTGGCACACAAGGTGCAAGGTTGCTCAGGGGACAGCAAATGGCATCAGGTTTCTGCATG
  AAAATCATCACATTCATAGAGATATTAAAAGTGCAAATATCTTACTAGACAGAGACTTTACTGCCAAAAT
  ATCTGACTTTGGGCTTGCACGGGCTTCGGCAAGGCTAGCGCAGACGGTCATGACCAGCCGAATCGTGGGC
  ACAACGGCTTACATGGCACCCGAAGCTTTGCGGGGAGAAATAACACCCAAATCTGACATCTACAGCTTCG
  GCGTGGTTCTGTTGGAGCTGATAACCGGGCTGGCGGCTGTGGATGAAAACCGTGAACCTCAACTACTGCT
  GGATATTAAAGAAGAGATTGAAGATGAAGAGAAGACGATTGAAGATTACACGGATGAGAAGATGAGCGAT
  GCGGACCCTGCTTCGGTGGAAGCAATGTACTCTGCTGCTAGCCAGTGTCTGCATGAGAAGAAAAACAGAC
  GGCCAGACATTGCAAAGGTTCAACAGCTGCTACAAGAGATGTCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207322 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNKPLTPSTYIRNLNVGILRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTCEL
  LFDWGTTNCTVGDLVDLLVQIELFAPATLLLPDAVPQTVKSLPPREAATVAQTHGPCQEKDRTSVMPMPK
  LEHSCEPPDSSSPDNRSVESSDTRFHSFSFHELKSITNNFDEQPASAGGNRMGEGGFGVVYKGCVNNTIV
  AVKKLGAMVEISTEELKQQFDQEIKVMATCQHENLVELLGFSSDSDNLCLVYAYMPNGSLLDRLSCLDGT
  PPLSWHTRCKVAQGTANGIRFLHENHHIHRDIKSANILLDRDFTAKISDFGLARASARLAQTVMTSRIVG
  TTAYMAPEALRGEITPKSDIYSFGVVLLELITGLAAVDENREPQLLLDIKEEIEDEEKTIEDYTDEKMSD
  ADPASVEAMYSAASQCLHEKKNRRPDIAKVQQLLQEMSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029926
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029926.2, NM_029926.3, NM_029926.4, NM_029926.5, NP_084202.2
• RefSeq Size: 2825 bp
• RefSeq ORF: 1380 bp
• Locus ID: 266632
• MW: 50.9 kDa