Fgd2 (BC021845) Mouse Tagged ORF Clone

CAT#: MR207373

Fgd2 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgd2
• Synonyms: ZFYVE4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207373 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAGCGAGTACGTGAAGAACTTTGAGCGGGCCGCGGAACTGCTGGCCACGTGGATGGACAAGTCTC
  AGCCCTTCCAGGAGGTGGTCACCCGCATCCAGTGCAGCGAGGCCTCCAGCAGCCTGACCCTGCAGCACCA
  CATGTTGGAGCCTGTGCAAAGAATCCCGCGGTACGAACTGCTGCTCAAGGAATATGTGCAGAAGCTGCCA
  GCCCAGGCCCCAGACCTCGAAGATGCCCAGAGAGCACTGGACATGATCTTCTCAGCTGCACAGCACTCCA
  ATGCAGCCATTGCAGAGATGGAGCGGCTGCAGGGCCTGTGGGATGTGTACCAGCGCCTGGGCTTGGAGGA
  TGACATCGTGGACCCCTCCAACACCCTGCTCCGAGAGGGCCCTGTTCTCAAGATCTCTTTCCGCCGCAGC
  GACCCAATGGAACGCTACCTGGTTTTGTTCAACAACATGCTTCTGTATTGTGTACCCCGAGTCCTCCAAG
  TGGGTGCCCAGTTCCAGGTGCGGACTCGCATCGATGTGGCCGGCATGAAGGTGCGGGAGCTGACCGATGC
  TGAGTTCCCACACTCCTTCCTGGTGTCCGGAAAGCAGCGCACACTGGAGCTGCAGGCCCGGTCCCGAGAT
  GAAATGGTTTCCTGGATGCAGGCCTGCCAGGCAGCCATTGACCAGGTTGAGAAGCGGAGTGAGACCTTCA
  AGGCTGCTGTCCAGGGACCTCAGGGGGACACGCAGGAGCCCAAGCCACAGGTAGAGGAGCTGGGTCTCCG
  AGCGCCTCAGTGGGTCCGGGACAAGATGGTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAATGCCCTG
  ACTCGTCGGCGCCACCACTGCCGGGCTTGCGGCTATGTAGTGTGTGCCAAGTGCTCTGACTACCGTGCGG
  AGCTGAAATATGACAGCAACAGGCCCAACCGAGTCTGCCTGACCTGCTACACATTTCTCACTGGAAACGT
  ACTCCCTCAAGGCAAGGAGGACAAGAGGCGGGGCATCTTGGAGAAAGAGGCCTCAGCAGCACCCGAGCAG
  AGTCTGGTGTGCAGCTTCCTGCAGCTCATAGGAGACAAGTGCAGCAGGAGCCTTCCCCGGAGTTGGTGTG
  TGATCCCCCGGGATGACCCCCTTGTCCTGTATGTCTATGCAGCCCCCCAGGACACAAAGGCTCACACCTC
  CATCCCCCTACTGGGCTATCAGGTGATTTCAGGGCCCCAGGGGGACCCTCGGGTTTTCCAGCTGCAACAG
  TCAGGCCAGCAGTACACCTTCAAGGCCGAGTCTGTGGAGCTGCAGGGCCGCTGGGTGACAGCTATCAAGC
  GTGCGGCCAGTGGCCGGACCCCTGAGGGACCTGACGAAGAAGATGTGTCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYSEYVKNFERAAELLATWMDKSQPFQEVVTRIQCSEASSSLTLQHHMLEPVQRIPRYELLLKEYVQKLP
  AQAPDLEDAQRALDMIFSAAQHSNAAIAEMERLQGLWDVYQRLGLEDDIVDPSNTLLREGPVLKISFRRS
  DPMERYLVLFNNMLLYCVPRVLQVGAQFQVRTRIDVAGMKVRELTDAEFPHSFLVSGKQRTLELQARSRD
  EMVSWMQACQAAIDQVEKRSETFKAAVQGPQGDTQEPKPQVEELGLRAPQWVRDKMVTMCMRCQEPFNAL
  TRRRHHCRACGYVVCAKCSDYRAELKYDSNRPNRVCLTCYTFLTGNVLPQGKEDKRRGILEKEASAAPEQ
  SLVCSFLQLIGDKCSRSLPRSWCVIPRDDPLVLYVYAAPQDTKAHTSIPLLGYQVISGPQGDPRVFQLQQ
  SGQQYTFKAESVELQGRWVTAIKRAASGRTPEGPDEEDVSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC021845
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021845, AAH21845
• RefSeq Size: 2094 bp
• RefSeq ORF: 1385 bp
• Locus ID: 26382
• MW: 52.5 kDa