Serpinc1 (NM_080844) Mouse Tagged ORF Clone

CAT#: MR207423

Serpinc1 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 (Serpinc1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Serpinc1
• Synonyms: AI114908; At-3; At3; ATIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207423 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCCCCTGGGGCAGGAAGTGGGGCTGCTGGTGAGAGGAAGCTTTGTCTCCTCTCTCTGCTCCTCA
  TCGGTGCCTTGGGCTGTGCTATCTGTCACGGAAACCCTGTGGACGACATCTGCATAGCGAAGCCCCGAGA
  CATCCCCGTGAATCCCTTGTGCATTTACCGCTCCCCTGGGAAGAAGGCCACCGAGGAGGATGGCTCAGAG
  CAGAAGGTTCCAGAAGCCACCAACCGGCGGGTCTGGGAACTGTCCAAGGCCAATTCGCGATTTGCCACTA
  ACTTCTACCAGCACCTGGCAGACTCCAAGAATGACAACGACAACATTTTCCTGTCACCCTTGAGCATCTC
  CACTGCTTTTGCTATGACCAAGCTGGGTGCCTGTAACGACACTCTCAAGCAGCTGATGGAGGTTTTTAAA
  TTTGATACCATCTCCGAGAAGACATCCGACCAGATCCACTTCTTCTTTGCCAAACTGAACTGCCGACTCT
  ATCGAAAAGCCAACAAGTCCTCTGACTTGGTATCAGCCAACCGCCTTTTTGGAGACAAATCCCTCACCTT
  CAACGAGAGCTATCAAGATGTTAGTGAGGTTGTCTATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGAG
  AATCCGGAGCAATCCAGAGTGACCATCAACAACTGGGTAGCTAATAAGACTGAAGGCCGCATCAAAGATG
  TCATCCCACAGGGCGCCATTAACGAGCTCACTGCCCTGGTTCTGGTTAACACCATTTACTTCAAGGGCCT
  GTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACCGTTCTATAAGGTCGATGGGCAGTCATGC
  CCAGTGCCTATGATGTACCAGGAAGGCAAATTCAAATACCGGCGCGTGGCAGAGGGCACCCAGGTGCTAG
  AGCTGCCCTTCAAGGGGGATGACATCACCATGGTGCTCATCCTGCCCAAGCCTGAGAAGAGCCTGGCCAA
  GGTGGAGCAGGAGCTCACCCCAGAGCTGCTGCAGGAGTGGCTGGATGAGCTGTCAGAGACTATGCTTGTG
  GTCCACATGCCCCGCTTCCGCACCGAGGATGGCTTCAGTCTGAAGGAGCAGCTGCAAGACATGGGCCTCA
  TTGATCTCTTCAGCCCTGAAAAGTCCCAACTCCCAGGGATCGTTGCTGGAGGCAGGGACGACCTCTATGT
  CTCCGACGCATTCCACAAAGCATTTCTTGAGGTAAATGAGGAAGGCAGTGAAGCAGCAGCGAGTACTTCT
  GTCGTGATTACTGGCCGGTCACTGAACCCCAATAGGGTGACCTTCAAGGCCAACAGGCCCTTCCTGGTTC
  TTATAAGGGAAGTTGCACTGAACACTATTATATTCATGGGGAGAGTGGCTAATCCTTGTGTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207423 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYSPGAGSGAAGERKLCLLSLLLIGALGCAICHGNPVDDICIAKPRDIPVNPLCIYRSPGKKATEEDGSE
  QKVPEATNRRVWELSKANSRFATNFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLKQLMEVFK
  FDTISEKTSDQIHFFFAKLNCRLYRKANKSSDLVSANRLFGDKSLTFNESYQDVSEVVYGAKLQPLDFKE
  NPEQSRVTINNWVANKTEGRIKDVIPQGAINELTALVLVNTIYFKGLWKSKFSPENTRKEPFYKVDGQSC
  PVPMMYQEGKFKYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEQELTPELLQEWLDELSETMLV
  VHMPRFRTEDGFSLKEQLQDMGLIDLFSPEKSQLPGIVAGGRDDLYVSDAFHKAFLEVNEEGSEAAASTS
  VVITGRSLNPNRVTFKANRPFLVLIREVALNTIIFMGRVANPCVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080844
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080844.1, NM_080844.2, NM_080844.3, NM_080844.4, NP_543120.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 1398 bp
• Locus ID: 11905
• MW: 52 kDa