Sil1 (NM_030749) Mouse Tagged ORF Clone

CAT#: MR207424

Sil1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum chaperone SIL1 homolog (S. cerevisiae) (Sil1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sil1
• Synonyms: 1810057E01Rik; AI042831; wz
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207424 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTCAGCACCTGCCTTCAACTAGGATGGCCTCTCCAGGAATGCTGCTGGGGCTGCTGTTGACTT
  CCTGTTTAACTCTCTGCCTCAGCTGTCAGAACTCAAATAATTTTGCACTCACCAACCCAGAGAAGAGCAT
  CCACCAGGAATCAGATACAAAGGAGACCAGGGAAGAGGAGGAGCTAGACACCGAGATCCTGGAGGTGTTC
  CACCCAACTCAAGAGTGGCAGACCCTTCAACCAGGTCAGGCTGTTCCTGCAGGATCCCATGTGCGAATGA
  ACCTACAGACTGGAGTAAACGAGGTGAAGCTCCAACAAGAAGACAAATTTCAAAATAATCTGAAAGGATT
  TAAAAGAGGCAGAAGGCTGGACATCAACGCCAACACATACACATCTCAGGACCTCAAGAGTGCGCTGGCC
  AAATTCAAGGAGGGGACCGAGATGGAGAATTCAAAGGATGAACTGGCGAGACAGGCCACAGTCAAGCAGC
  TCTTCCGCCCCATTGAGGAGCTGAAGAAGGAATTTGATGAGCTGAACGTTGTTCTTGAGACTGACATGCA
  GATTATGGTTCGGCTGATAAACAAGTTCAACAGCTCCAGCTCCAGCCTAGAAGAGAAGGTTGCTGCGCTC
  TTTGATCTTGAGTACTATGTCCATCAGATGGATAACGCACAGGACCTGCTGTCCTTTGGTGGCCTTCAAG
  TGGTGATTAATGGGCTCAACAGCACAGAACCCCTGGTGAAGGAGTATGCTGCTTTTGTGCTGGGGGCCGC
  CTTTTCCAGCAATCCGAAGGTCCAGGTGGAAGCCATCGAAGGGGGAGCCCTGCAGAAGCTGCTGGTCATC
  CTGGCCACAAACCAGCCTCTCCCCGCCAAGAAGAAGGTCCTGTTTGCACTGTGCTCCCTGCTGCGCCACT
  TCCCCTATGCCCAGCAGCAGTTCCTGAAGCTCGGGGGCCTGCAGGTCCTCAGGAGCCTGGTACAGGAGAA
  GAGTGCGAAGGTACTGGCCGTGCGTGTGGTCACCCTGCTCTATGACCTGGTCACTGAGAAGATGTTTGCT
  GAGGAGGAGGCAGAGTTGACCCAGGATTCATCCCCAGAGAAGCTGCAGCAGTATCGCCAGGTGCAGCTCC
  TGCCAGGCCTGCAGGAACAGGGTTGGTGTGAGATCACCGCCCAGCTCCTGGCCCTTCCAGAGCATGATGC
  TCGGGAGAAGGTGCTGCAGACACTGGGTGCCCTCCTGACCACCTGCCGGGATCGCTACCGTCAGGACCTC
  CAGTTGAGCAGGACACTAGGGCGTCTCCAAGCAGAGTACCAAGCCCTGGCCTCCCTGGAGCTCCAAGAAG
  GCGAAGATGATGGCTACTTCCGGGAACTGCTGGCCTCCATCAACAGCTTGATGAAGGAACTAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207424 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPQHLPSTRMASPGMLLGLLLTSCLTLCLSCQNSNNFALTNPEKSIHQESDTKETREEEELDTEILEVF
  HPTQEWQTLQPGQAVPAGSHVRMNLQTGVNEVKLQQEDKFQNNLKGFKRGRRLDINANTYTSQDLKSALA
  KFKEGTEMENSKDELARQATVKQLFRPIEELKKEFDELNVVLETDMQIMVRLINKFNSSSSSLEEKVAAL
  FDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVI
  LATNQPLPAKKKVLFALCSLLRHFPYAQQQFLKLGGLQVLRSLVQEKSAKVLAVRVVTLLYDLVTEKMFA
  EEEAELTQDSSPEKLQQYRQVQLLPGLQEQGWCEITAQLLALPEHDAREKVLQTLGALLTTCRDRYRQDL
  QLSRTLGRLQAEYQALASLELQEGEDDGYFRELLASINSLMKELR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030749
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030749.1, NM_030749.2, NP_109674.1, NP_109674.2
• RefSeq Size: 1715 bp
• RefSeq ORF: 1398 bp
• Locus ID: 81500
• MW: 52.4 kDa