Psen1 (NM_008943) Mouse Tagged ORF Clone

CAT#: MR207460

Psen1 (Myc-DDK-tagged) - Mouse presenilin 1 (Psen1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psen1
• Synonyms: Ad3h; PS-1; PS1; S182
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207460 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGAGATACCTGCACCTTTGTCCTACTTCCAGAATGCCCAGATGTCTGAGGACAGCCACTCCAGCA
  GCGCCATCCGGAGCCAGAATGACAGCCAAGAACGGCAGCAGCAGCATGACAGGCAGAGACTTGACAACCC
  TGAGCCAATATCTAATGGGCGGCCCCAGAGTAACTCAAGACAGGTGGTGGAACAAGATGAGGAGGAAGAC
  GAAGAGCTGACATTGAAATATGGAGCCAAGCATGTCATCATGCTCTTTGTCCCCGTGACCCTCTGCATGG
  TCGTCGTCGTGGCCACCATCAAATCAGTCAGCTTCTATACCCGGAAGGACGGTCAGCTAATCTACACCCC
  ATTCACAGAAGACACTGAGACTGTAGGCCAAAGAGCCCTGCACTCGATCCTGAATGCGGCCATCATGATC
  AGTGTCATTGTCATTATGACCATCCTCCTGGTGGTCCTGTATAAATACAGGTGCTACAAGGTCATCCACG
  CCTGGCTTATTATTTCATCTCTGTTGTTGCTGTTCTTTTTTTCGTTCATTTACTTAGGGGAAGTATTTAA
  GACCTACAATGTCGCCGTGGACTACGTTACAGTAGCACTCCTAATCTGGAATTTTGGTGTGGTCGGGATG
  ATTGCCATCCACTGGAAAGGCCCCCTTCGACTGCAGCAGGCGTATCTCATTATGATCAGTGCCCTCATGG
  CCCTGGTATTTATCAAGTACCTCCCCGAATGGACCGCATGGCTCATCTTGGCTGTGATTTCAGTATATGA
  TTTGGTGGCTGTTTTATGTCCCAAAGGCCCACTTCGTATGCTGGTTGAAACAGCTCAGGAAAGAAATGAG
  ACTCTCTTTCCAGCTCTTATCTATTCCTCAACAATGGTGTGGTTGGTGAATATGGCTGAAGGAGACCCAG
  AAGCCCAAAGGAGGGTACCCAAGAACCCCAAGTATAACACACAAAGAGCGGAGAGAGAGACACAGGACAG
  TGGTTCTGGGAACGATGATGGTGGCTTCAGTGAGGAGTGGGAGGCCCAAAGAGACAGTCACCTGGGGCCT
  CATCGCTCCACTCCCGAGTCAAGAGCTGCTGTCCAGGAACTTTCTGGGAGCATTCTAACGAGTGAAGACC
  CGGAGGAAAGAGGAGTAAAACTTGGACTGGGAGATTTCATTTTCTACAGTGTTCTGGTTGGTAAGGCCTC
  AGCAACCGCCAGTGGAGACTGGAACACAACCATAGCCTGCTTTGTAGCCATACTGATCGGCCTGTGCCTT
  ACATTACTCCTGCTCGCCATTTTCAAGAAAGCGTTGCCAGCCCTCCCCATCTCCATCACCTTCGGGCTCG
  TGTTCTACTTCGCCACGGATTACCTTGTGCAGCCCTTCATGGACCAACTTGCATTCCATCAGTTTTATAT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207460 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEIPAPLSYFQNAQMSEDSHSSSAIRSQNDSQERQQQHDRQRLDNPEPISNGRPQSNSRQVVEQDEEED
  EELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMI
  SVIVIMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYVTVALLIWNFGVVGM
  IAIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNE
  TLFPALIYSSTMVWLVNMAEGDPEAQRRVPKNPKYNTQRAERETQDSGSGNDDGGFSEEWEAQRDSHLGP
  HRSTPESRAAVQELSGSILTSEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCL
  TLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008943
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008943.1, NM_008943.2, NP_032969.1
• RefSeq Size: 3023 bp
• RefSeq ORF: 1404 bp
• Locus ID: 19164
• MW: 52.6 kDa