Des (NM_010043) Mouse Tagged ORF Clone

CAT#: MR207502

Des (Myc-DDK-tagged) - Mouse desmin (Des)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Des
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207502 representing NM_010043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGGCCTACTCGTCCAGCCAGCGCGTGTCCTCCTACCGCCGCACCTTCGGCGGCGCCCCGGGCT
  TCTCTCTGGGCTCCCCGCTGAGCTCTCCCGTGTTCCCTCGAGCAGGCTTCGGTACCAAGGGCTCCTCGAG
  TTCAATGACATCCCGCGTGTACCAGGTGTCGCGCACGTCGGGCGGGGCTGGAGGCTTGGGGTCGCTGCGG
  TCTAGCCGGCTGGGGACCACCCGAGCGCCATCCTATGGCGCGGGCGAGCTGCTGGACTTCTCCCTGGCCG
  ACGCTGTGAACCAGGAGTTCCTGGCCACGCGCACCAACGAGAAGGTGGAGCTGCAAGAGCTCAATGACCG
  CTTCGCCAACTACATCGAGAAGGTGCGCTTCTTGGAGCAGCAGAACGCCGCGCTCGCCGCCGAGGTCAAC
  CGGCTCAAGGGCCGCGAACCGACTCGGGTCGCCGAGCTCTACGAGGAGGAGATGCGCGAGCTGCGGCGCC
  AGGTGGAGGTGCTCACCAACCAGCGCGCCCGGGTCGACGTGGAGCGTGACAACCTGATAGACGACCTGCA
  GAGGCTCAAGGCCAAACTACAGGAGGAAATCCAACTAAGAGAAGAAGCAGAGAACAACTTGGCTGCCTTC
  CGAGCGGATGTGGATGCAGCCACTCTAGCTCGTATTGACCTGGAGCGCAGAATCGAATCCCTCAACGAGG
  AGATCGCGTTCCTTAAGAAAGTGCATGAAGAGGAGATCCGTGAGCTTCAGGCCCAGCTTCAGGAACAGCA
  GGTCCAGGTGGAGATGGACATGTCCAAGCCGGACCTCACAGCTGCCCTCAGGGACATCCGGGCTCAGTAT
  GAGACCATCGCGGCTAAGAACATCTCTGAGGCTGAAGAATGGTACAAGTCCAAGGTTTCAGACTTGACTC
  AGGCAGCCAATAAGAACAACGATGCGCTGCGCCAAGCCAAGCAGGAGATGATGGAATACCGACACCAGAT
  CCAGTCCTACACCTGCGAGATTGATGCCCTCAAGGGCACCAACGACTCCCTGATGAGGCAGATGAGGGAG
  CTGGAGGATCGCTTTGCCAGCGAGGCCAATGGCTATCAGGACAACATTGCGCGCCTGGAGGAGGAGATCC
  GACACCTAAAGGATGAGATGGCCCGCCATCTGCGCGAGTACCAGGACCTGCTCAATGTGAAGATGGCCTT
  GGATGTGGAGATCGCCACCTACCGGAAGCTACTGGAGGGCGAGGAGAGCAGGATCAACCTTCCTATCCAG
  ACCTTCTCTGCTCTCAACTTCCGAGAAACCAGCCCCGAGCAAAGGGGTTCTGAAGTCCATACCAAAAAGA
  CAGTGATGATCAAGACCATTGAGACCCGGGATGGAGAGGTTGTCAGCGAGGCTACACAGCAACAACATGA
  AGTGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207502 representing NM_010043
  Red=Cloning site Green=Tags(s)
  
  MSQAYSSSQRVSSYRRTFGGAPGFSLGSPLSSPVFPRAGFGTKGSSSSMTSRVYQVSRTSGGAGGLGSLR
  SSRLGTTRAPSYGAGELLDFSLADAVNQEFLATRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVN
  RLKGREPTRVAELYEEEMRELRRQVEVLTNQRARVDVERDNLIDDLQRLKAKLQEEIQLREEAENNLAAF
  RADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQY
  ETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRE
  LEDRFASEANGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQ
  TFSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010043
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010043.1, NM_010043.2, NP_034173.1
• RefSeq Size: 3065
• RefSeq ORF: 1410
• Locus ID: 13346
• MW: 53.5 kDa
• Gene Summary: This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane and are essential for maintaining the strength and integrity of skeletal, cardiac and smooth muscle fibers. Mutations in this gene affect assembly of intermediate filaments. Mice lacking this gene are able to develop and reproduce but exhibit abnormal muscle fibers. Mutations in the human gene are associated with myofibrillar myopathy, dilated cardiomyopathy, neurogenic scapuloperoneal syndrome and autosomal recessive limb-girdle muscular dystrophy, type 2R. [provided by RefSeq, Jan 2014]