Nono (NM_023144) Mouse Tagged ORF Clone

CAT#: MR207550

Nono (Myc-DDK-tagged) - Mouse non-POU-domain-containing, octamer binding protein (Nono)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nono
• Synonyms: AA407051; AV149256; nonA; NRB54; P54NRB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207550 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCAATAAAGCCTTTAACTTGGAGAAGCAGAATCATACTCCAAGGAAGCATCATCAGCATCACC
  ACCAGCAGCACCATCAGCAGCAACAGCAGCAGCAGCAGCAACAGCCACCCCCACCAATACCTGCAAATGG
  CCAGCAGGCCAGCAGCCAGAATGAAGGCTTGACTATTGACCTGAAGAATTTTAGGAAACCAGGAGAGAAG
  ACCTTTACACAGCGTAGCCGTCTCTTTGTGGGCAATCTTCCCCCTGATATCACTGAGGAGGAAATGAGGA
  AACTATTTGAGAAATATGGAAAAGCAGGCGAAGTTTTCATTCATAAGGATAAAGGCTTTGGCTTTATTCG
  CTTGGAAACACGAACCCTAGCGGAAATTGCCAAAGTGGAGCTGGACAACATGCCCCTCCGTGGGAAGCAG
  CTGCGAGTGCGCTTTGCCTGTCACAGTGCATCCCTTACAGTCCGCAACCTTCCTCAGTACGTGTCGAACG
  AACTGCTGGAAGAAGCCTTTTCTGTGTTCGGCCAGGTGGAGAGGGCTGTAGTCATTGTGGATGACCGAGG
  AAGGCCCTCAGGGAAAGGCATTGTTGAGTTCTCAGGGAAGCCAGCTGCTCGGAAAGCTCTGGACAGATGC
  AGTGAAGGCTCCTTCTTGCTGACTACATTTCCTCGGCCTGTGACTGTGGAGCCTATGGACCAGTTAGATG
  ATGAAGAGGGACTTCCAGAGAAACTGGTTATAAAAAACCAGCAATTCCACAAGGAGAGAGAACAGCCACC
  CAGATTTGCACAACCTGGCTCCTTTGAGTATGAGTATGCCATGCGCTGGAAGGCACTCATTGAGATGGAG
  AAGCAACAGCAGGATCAAGTGGATCGGAACATCAAGGAGGCTCGTGAGAAGCTGGAGATGGAGATGGAGG
  CTGCACGTCATGAGCACCAGGTTATGCTAATGAGGCAGGATTTGATGAGACGTCAAGAAGAGCTTCGGAG
  AATGGAGGAGCTGCATAACCAAGAGGTTCAGAAGCGAAAGCAGTTAGAACTCAGGCAGGAAGAGGAACGC
  AGGCGCCGTGAGGAAGAGATGCGGCGACAGCAAGAGGAAATGATGCGCCGACAGCAGGAAGGATTCAAGG
  GAACCTTCCCTGATGCGAGAGAACAAGAGATACGGATGGGCCAAATGGCTATGGGAGGTGCTATGGGCAT
  AAACAATAGAGGCGCGATGCCCCCTGCTCCTGTGCCACCTGGTACTCCAGCTCCTCCAGGACCTGCCACT
  ATGATGCCAGATGGAACCCTTGGATTGACCCCACCAACAACTGAACGTTTTGGCCAAGCTGCAACAATGG
  AAGGAATTGGAGCAATTGGTGGAACTCCTCCTGCATTCAACCGTCCAGCTCCGGGAGCTGAATTTGCTCC
  AAATAAACGCCGCCGATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207550 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQSNKAFNLEKQNHTPRKHHQHHHQQHHQQQQQQQQQQPPPPIPANGQQASSQNEGLTIDLKNFRKPGEK
  TFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQ
  LRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRC
  SEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEME
  KQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEER
  RRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPPGTPAPPGPAT
  MMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRPAPGAEFAPNKRRRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023144
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023144.1, NM_023144.2, NP_075633.1, NP_075633.2
• RefSeq Size: 2468 bp
• RefSeq ORF: 1422 bp
• Locus ID: 53610
• MW: 54.5 kDa