Cnr1 (NM_007726) Mouse Tagged ORF Clone

CAT#: MR207569

Cnr1 (Myc-DDK-tagged) - Mouse cannabinoid receptor 1 (brain) (Cnr1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnr1
• Synonyms: CB-R; CB1; CB1A; CB1B; CB1R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCGATCTTAGACGGCCTTGCAGATACCACCTTCCGTACCATCACCACAGACCTCCTCTACGTGG
  GCTCAAATGACATTCAGTACGAAGATATCAAAGGAGACATGGCATCCAAATTAGGATACTTCCCACAGAA
  ATTCCCTCTAACTTCCTTCAGGGGTAGTCCCTTCCAAGAAAAGATGACGGCAGGAGACAACTCCCCGTTG
  GTTCCAGCAGGAGACACAACCAACATTACAGAGTTCTATAACAAGTCTCTCTCATCGTTCAAGGAGAACG
  AGGACAACATCCAGTGTGGGGAGAATTTTATGGACATGGAGTGCTTCATGATTCTGAATCCCAGCCAGCA
  GCTGGCCATCGCTGTCCTGTCCCTCACCCTGGGCACCTTCACGGTTCTGGAGAACCTGCTGGTGCTATGT
  GTCATCCTTCACTCCCGCAGTCTCCGATGCAGGCCTTCCTACCACTTCATTGGCAGCCTGGCGGTGGCCG
  ATCTCCTGGGAAGTGTCATCTTTGTCTACAGCTTTGTTGACTTCCACGTGTTCCACCGCAAAGATAGTCC
  CAATGTGTTTCTGTTCAAACTGGGTGGGGTTACCGCCTCCTTCACAGCATCTGTGGGCAGCCTGTTCCTC
  ACGGCCATCGACAGGTACATATCCATTCACAGGCCTCTGGCCTATAAGAGGATCGTCACCAGGCCCAAGG
  CCGTAGTGGCCTTTTGCTTGATGTGGACTATTGCAATAGTAATTGCTGTGTTGCCTCTCCTGGGCTGGAA
  CTGCAAGAAGCTGCAATCTGTTTGCTCAGACATCTTCCCACTCATTGATGAAACCTACCTGATGTTCTGG
  ATCGGAGTCACCAGTGTGCTGTTGCTGTTCATTGTGTATGCATACATGTACATTCTCTGGAAGGCTCACA
  GCCACGCAGTTCGCATGATCCAGCGTGGAACCCAGAAAAGCATCATCATTCACACCTCAGAAGATGGCAA
  GGTGCAGGTGACACGGCCTGACCAAGCCCGCATGGACATTAGGCTGGCCAAAACCCTGGTTCTGATCCTG
  GTGGTGTTGATCATCTGCTGGGGCCCTCTGCTTGCGATCATGGTGTATGATGTCTTTGGGAAGATGAACA
  AGCTTATCAAGACGGTGTTTGCCTTCTGTAGTATGCTCTGCCTGCTGAACTCCACCGTGAACCCCATCAT
  CTATGCTCTGAGGAGCAAGGACCTGAGACATGCTTTCCGCAGCATGTTCCCTTCATGTGAAGGCACTGCG
  CAGCCTCTAGATAACAGCATGGGGGACTCAGACTGCCTGCACAAGCACGCCAATAACACAGCCAGCATGC
  ACAGGGCCGCGGAAAGCTGCATCAAGAGCACTGTTAAGATCGCCAAGGTGACCATGTCTGTGTCCACAGA
  CACGTCTGCCGAGGCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNSPL
  VPAGDTTNITEFYNKSLSSFKENEDNIQCGENFMDMECFMILNPSQQLAIAVLSLTLGTFTVLENLLVLC
  VILHSRSLRCRPSYHFIGSLAVADLLGSVIFVYSFVDFHVFHRKDSPNVFLFKLGGVTASFTASVGSLFL
  TAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCKKLQSVCSDIFPLIDETYLMFW
  IGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQVTRPDQARMDIRLAKTLVLIL
  VVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLLNSTVNPIIYALRSKDLRHAFRSMFPSCEGTA
  QPLDNSMGDSDCLHKHANNTASMHRAAESCIKSTVKIAKVTMSVSTDTSAEAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007726
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007726.1, NM_007726.2, NM_007726.3, NP_031752.1
• RefSeq Size: 5807 bp
• RefSeq ORF: 1422 bp
• Locus ID: 12801
• MW: 52.8 kDa
• Gene Summary: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. [UniProtKB/Swiss-Prot Function]