Kcns2 (NM_181317) Mouse Tagged ORF Clone

CAT#: MR207651

Kcns2 (Myc-DDK-tagged) - Mouse K+ voltage-gated channel, subfamily S, 2 (Kcns2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcns2
• Synonyms: E130006J24Rik; Kv9.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207651 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCGCCAGAGCCTGTGGGATGTGTCCGATACCGACGTCGAGGATGGAGAGATCCGCATCAATGTGG
  GTGGCTTCAAGAGACGGCTGCGTTCCCATACGCTGCTGCGCTTCCCTGAGACACGCCTGGGCCGTCTGCT
  CCTCTGCCACTCGCGAGAGGCCATTCTGGAACTCTGCGATGACTACGATGACGTTCAGCGTGAGTTCTAC
  TTCGACCGTAACCCCGAGCTCTTCCCCTATGTGTTGCATTTCTACCACACCGGCAAGCTTCACGTCATGG
  CTGAGCTGTGCGTCTTCTCCTTCAGCCAGGAGATCGAGTACTGGGGTATCAATGAGTTCTTCATCGACTC
  TTGCTGCAGCTATAGCTATCACGGCCGCAAAGTGGAACCTGAGCAGGAGAAATGGGACGAGCAGAGTGAC
  CAGGAAAGCACCACTTCCTCCTTCGATGAGATCTTGGCCTTCTATAATGATGCTTCCAAGTTCGATGGGC
  AACCCCTGGGCAACTTCCGCAGGCAGCTGTGGCTGGCGTTGGACAACCCAGGCTACTCAGTCCTAAGCAG
  GGTCTTCAGTGTCCTTTCCATCTTGGTGGTGTTGGGCTCCATCATCACCATGTGCCTCAATAGCCTGCCA
  GACTTCCAAATCCCTGATAGCCAGGGTAACCCCGGTGAAGACCCCAGGTTCGAAATTGTGGAGCACTTTG
  GCATTGCTTGGTTCACATTTGAGTTGGTGGCCAGGTTTGCTGTGGCCCCTGACTTTCTTAAGTTCTTCAA
  GAATGCTCTAAACCTTATTGATCTCATGTCCATTGTCCCATTTTACATAACTCTAGTGGTGAACCTGGTG
  GTGGAGAGTTCTCCTACCTTGGCTAACTTGGGCAGGGTGGCTCAAGTCCTGAGGCTAATGAGGATCTTCC
  GAATTCTCAAGCTGGCCAGACACTCCACTGGCCTCCGCTCCTTGGGAGCCACCCTGAAGTACAGCTACAA
  GGAAGTGGGGTTGCTCTTGCTCTACCTCTCAGTGGGGATTTCCATCTTCTCTGTGGTGGCCTACACCATT
  GAAAAGGAGGAGAACGAAGGCCTGGCCACCATCCCTGCCTGCTGGTGGTGGGCCACTGTCAGTATGACCA
  CAGTTGGGTACGGAGATGTGGTCCCAGGGACAACAGCTGGGAAGTTGACTGCCTCTGCCTGCATCTTGGC
  AGGCATCCTGGTGGTGGTCTTGCCCATCACTTTGATCTTCAATAAGTTCTCCCATTTCTATCGGCGCCAA
  AAGCAACTTGAGAGTGCTATGCGCAGCTGTGACTTTGGAGATGGAATGAAAGAGGTCCCTTCGGTCAATT
  TAAGGGACTACTATGCTCATAAAGTTAAGTCCCTCATGGCAAGTCTGACAAACATGAGTAGGAGTTCACC
  TAGTGAACTGAGTTTAGATGATTCTCTACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207651 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTRQSLWDVSDTDVEDGEIRINVGGFKRRLRSHTLLRFPETRLGRLLLCHSREAILELCDDYDDVQREFY
  FDRNPELFPYVLHFYHTGKLHVMAELCVFSFSQEIEYWGINEFFIDSCCSYSYHGRKVEPEQEKWDEQSD
  QESTTSSFDEILAFYNDASKFDGQPLGNFRRQLWLALDNPGYSVLSRVFSVLSILVVLGSIITMCLNSLP
  DFQIPDSQGNPGEDPRFEIVEHFGIAWFTFELVARFAVAPDFLKFFKNALNLIDLMSIVPFYITLVVNLV
  VESSPTLANLGRVAQVLRLMRIFRILKLARHSTGLRSLGATLKYSYKEVGLLLLYLSVGISIFSVVAYTI
  EKEENEGLATIPACWWWATVSMTTVGYGDVVPGTTAGKLTASACILAGILVVVLPITLIFNKFSHFYRRQ
  KQLESAMRSCDFGDGMKEVPSVNLRDYYAHKVKSLMASLTNMSRSSPSELSLDDSLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181317
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181317.1, NM_181317.2, NM_181317.3, NM_181317.4, NP_851834.1
• RefSeq Size: 5473 bp
• RefSeq ORF: 1434 bp
• Locus ID: 16539
• MW: 54.3 kDa