Vtn (NM_011707) Mouse Tagged ORF Clone

CAT#: MR207667

Vtn (Myc-DDK-tagged) - Mouse vitronectin (Vtn)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vtn
• Synonyms: AI256434; Vn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207667 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCCCTGAGGCCCTTTTTCATACTAGCCCTGGTGGCATGGGTTTCTCTGGCTGACCAAGAGTCAT
  GCAAGGGCCGCTGCACTCAGGGTTTCATGGCCAGCAAGAAGTGTCAGTGTGACGAGCTTTGCACTTACTA
  TCAGAGCTGCTGTGCCGACTACATGGAGCAGTGCAAGCCCCAAGTAACGCGGGGGGACGTGTTCACTATG
  CCAGAGGATGATTATTGGAGCTATGACTACGTGGAGGAGCCCAAGAACAATACCAACACCGGTGTGCAAC
  CCGAGAACACCTCTCCACCCGGTGACCTAAATCCTCGGACGGACGGCACTCTAAAGCCGACAGCCTTCCT
  AGATCCTGAGGAACAGCCAAGCACCCCAGCGCCTAAAGTGGAGCAACAGGAGGAGATCCTAAGGCCCGAC
  ACCACTGATCAAGGGACCCCTGAGTTTCCAGAGGAAGAACTGTGCAGTGGAAAGCCCTTTGACGCCTTCA
  CGGATCTCAAGAATGGGTCCCTCTTTGCCTTCCGAGGGCAGTACTGCTATGAGCTAGATGAGACGACAGT
  GAGGCCTGGGTACCCCAAACTTATCCAAGATGTCTGGGGCATTGAGGGCCCCATCGATGCTGCCTTCACT
  CGCATCAACTGTCAGGGGAAGACCTACTTGTTCAAGGGTAGTCAGTACTGGCGCTTTGAGGATGGGGTCC
  TGGACCCTGGTTATCCCCGAAACATCTCCGAAGGCTTCAGTGGCATACCAGACAATGTTGATGCAGCGTT
  CGCCCTTCCTGCCCACCGTTACAGTGGCCGGGAAAGGGTCTACTTCTTCAAGGGGAAGCAGTACTGGGAG
  TACGAATTTCAGCAGCAACCCAGCCAGGAGGAGTGCGAAGGCAGCTCTCTGTCAGCCGTGTTTGAGCACT
  TTGCCTTGCTTCAGCGGGACAGCTGGGAGAACATTTTCGAACTCCTCTTCTGGGGCAGATCCTCTGATGG
  AGCCAGAGAACCCCAATTCATCAGCCGGAACTGGCATGGTGTGCCAGGGAAAGTGGACGCTGCTATGGCC
  GGCCGCATCTACGTCACTGGCTCCTTATCCCACTCTGCCCAAGCCAAAAAACAGAAGTCTAAGCGTAGAA
  GCCGAAAGCGCTATCGTTCACGCCGAGGGCGTGGCCACAGACGCAGCCAGAGCTCGAACTCCCGTCGTTC
  ATCACGTTCAATCTGGTTCTCTTTGTTCTCCAGCGAGGAGAGTGGGCTAGGAACCTACAACAACTATGAT
  TATGATATGGACTGGCTTGTACCTGCCACCTGCGAGCCCATTCAGAGCGTCTATTTCTTCTCTGGAGACA
  AATACTACCGAGTCAACCTTAGAACCCGGCGAGTGGACTCTGTGAATCCTCCCTACCCACGCTCCATTGC
  TCAGTATTGGCTGGGCTGCCCGACCTCTGAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207667 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPLRPFFILALVAWVSLADQESCKGRCTQGFMASKKCQCDELCTYYQSCCADYMEQCKPQVTRGDVFTM
  PEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLNPRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPD
  TTDQGTPEFPEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDETTVRPGYPKLIQDVWGIEGPIDAAFT
  RINCQGKTYLFKGSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWE
  YEFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHGVPGKVDAAMA
  GRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSRSIWFSLFSSEESGLGTYNNYD
  YDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSVNPPYPRSIAQYWLGCPTSEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011707
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011707.1, NM_011707.2, NP_035837.1
• RefSeq Size: 1763 bp
• RefSeq ORF: 1437 bp
• Locus ID: 22370
• MW: 54.9 kDa
• Gene Summary: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. [UniProtKB/Swiss-Prot Function]