Edil3 (NM_001037987) Mouse Tagged ORF Clone

CAT#: MR207688

Edil3 (Myc-DDK-tagged) - Mouse EGF-like repeats and discoidin I-like domains 3 (Edil3), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Edil3
• Synonyms: Del-1; Del1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207688 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCACTTGGTAGCAGCCTGGCTTTTGGTTGGACTCAGCCTCGGGGTGCCCCAGTTCGGCAAAGGTG
  ACATTTGCAACCCGAACCCCTGTGAAAATGGTGGCATCTGTCTGTCAGGACTGGCTGATGATTCCTTTTC
  CTGTGAGTGTCCAGAAGGCTTCGCAGGTCCGAACTGCTCTAGTGTTGTGGAGGTTGCATCAGATGAAGAA
  AAGCCTACTTCAGCAGGTCCCTGCATCCCTAACCCATGCCATAACGGAGGAACCTGTGAGATAAGCGAAG
  CCTATCGAGGAGACACATTCATAGGCTATGTTTGTAAATGTCCTCGGGGATTTAATGGGATTCACTGTCA
  GCACAATATAAATGAATGTGAAGCTGAGCCTTGCAGAAATGGCGGAATATGTACCGACCTTGTTGCTAAC
  TACTCTTGTGAATGCCCAGGAGAATTTATGGGACGAAATTGTCAATATAAATGCTCTGGGCCATTGGGAA
  TCGAAGGTGGGATCATATCTAATCAGCAAATCACAGCTTCATCTACTCACCGAGCTCTTTTTGGACTCCA
  GAAGTGGTATCCCTACTATGCTCGACTTAATAAGAAGGGCCTTATAAATGCCTGGACAGCTGCTGAAAAT
  GACAGATGGCCATGGATTCAGATAAATTTGCAAAGAAAAATGAGAGTCACTGGTGTTATTACCCAAGGAG
  CAAAAAGGATTGGAAGCCCAGAGTACATAAAATCCTACAAAATTGCCTACAGCAATGACGGGAAGACCTG
  GGCAATGTACAAAGTAAAAGGCACCAATGAAGAGATGGTCTTTCGTGGAAATGTTGATAACAACACACCA
  TATGCTAATTCTTTCACACCCCCAATCAAAGCTCAGTATGTAAGACTCTACCCCCAAATTTGTCGAAGGC
  ATTGTACTTTAAGAATGGAACTTCTTGGCTGTGAGCTCTCAGGCTGTTCAGAACCTTTGGGGATGAAATC
  AGGGCATATACAAGACTACCAGATCACTGCCTCCAGCGTCTTCAGAACACTCAACATGGACATGTTTACT
  TGGGAACCAAGGAAAGCCAGGCTGGACAAGCAAGGCAAAGTAAATGCCTGGACTTCCGGCCATAACGACC
  AGTCACAATGGTTACAGGTTGATCTTCTTGTCCCTACTAAGGTGACAGGCATCATTACACAAGGAGCTAA
  AGATTTTGGTCACGTGCAGTTTGTTGGGTCATACAAACTAGCTTACAGCAATGATGGAGAACACTGGATG
  GTGCACCAGGATGAAAAACAGAGAAAAGACAAGGTTTTTCAAGGCAATTTTGACAATGACACTCACAGGA
  AAAATGTCATCGACCCTCCCATCTATGCACGATTCATAAGAATCCTTCCTTGGTCCTGGTATGGAAGGAT
  CACTCTGCGGTCAGAGCTGCTGGGCTGCGCAGAGGAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207688 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKHLVAAWLLVGLSLGVPQFGKGDICNPNPCENGGICLSGLADDSFSCECPEGFAGPNCSSVVEVASDEE
  KPTSAGPCIPNPCHNGGTCEISEAYRGDTFIGYVCKCPRGFNGIHCQHNINECEAEPCRNGGICTDLVAN
  YSCECPGEFMGRNCQYKCSGPLGIEGGIISNQQITASSTHRALFGLQKWYPYYARLNKKGLINAWTAAEN
  DRWPWIQINLQRKMRVTGVITQGAKRIGSPEYIKSYKIAYSNDGKTWAMYKVKGTNEEMVFRGNVDNNTP
  YANSFTPPIKAQYVRLYPQICRRHCTLRMELLGCELSGCSEPLGMKSGHIQDYQITASSVFRTLNMDMFT
  WEPRKARLDKQGKVNAWTSGHNDQSQWLQVDLLVPTKVTGIITQGAKDFGHVQFVGSYKLAYSNDGEHWM
  VHQDEKQRKDKVFQGNFDNDTHRKNVIDPPIYARFIRILPWSWYGRITLRSELLGCAEEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037987
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037987.1, NM_001037987.2, NM_001037987.3, NP_001033076.1
• RefSeq Size: 5358 bp
• RefSeq ORF: 1443 bp
• Locus ID: 13612
• Cytogenetics: 13 C3
• MW: 53.7 kDa
• Gene Summary: Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development. [UniProtKB/Swiss-Prot Function]