Ldhd (NM_027570) Mouse Tagged ORF Clone

CAT#: MR207759

Ldhd (Myc-DDK-tagged) - Mouse lactate dehydrogenase D (Ldhd), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldhd
• Synonyms: 4733401P21Rik; D8Bwg1320e; DLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207759 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGCTCCTTCGGGTGGCCACCCAGAGGCTGTCTCCCTGGAGGAGTTTCTGCTCCAGGGGGTCGC
  AGGGTGGACTCAGCCAGGACTTTGTGGAGGCTCTGAAGGCAGTTGTAGGGAGCCCCCACGTGTCCACTGC
  TTCTGCAGTCAGAGAGCAACACGGGCATGATGAATCGATGCACAGGTGTCAACCTCCTGATGCTGTGGTT
  TGGCCTCAGAATGTGGACCAGGTCAGCCGAGTGGCAAGCCTGTGCTACAATCAAGGTGTTCCCATCATCC
  CATTTGGCACAGGCACCGGTGTTGAGGGGGGAGTCTGTGCTGTGCAGGGTGGAGTGTGCATCAACCTGAC
  CCATATGGACCAGATCACGGAGCTGAATACAGAAGACTTCTCCGTGGTGGTGGAGCCTGGAGTCACCCGT
  AAAGCTCTCAATACCCACCTGCGTGACAGTGGCCTTTGGTTTCCTGTTGACCCAGGCGCAGATGCTTCTC
  TGTGTGGCATGGCAGCCACCGGGGCCTCGGGCACCAATGCTGTGCGCTATGGAACCATGCGGGACAACGT
  GATTAACCTGGAGGTAGTGCTGCCTGACGGCAGGCTGCTTCACACCGCAGGCCGGGGCCGCCATTACAGG
  AAGAGTGCAGCTGGCTACAATCTCACAGGACTCTTTGTGGGCTCTGAGGGGACCCTGGGCATCATTACAT
  CAACCACCCTGCGCCTGCACCCTGCGCCGGAAGCAACGGTGGCTGCCACCTGTGCGTTCCCCAGTGTTCA
  AGCGGCTGTGGACAGCACAGTTCAGATCCTCCAGGCTGCAGTGCCCGTGGCTCGCATTGAGTTCCTAGAT
  GATGTCATGATGGATGCCTGCAACAGACACAGCAAACTTAACTGCCCCGTGGCACCCACGCTTTTCCTGG
  AGTTCCACGGTTCCCAGCAGACACTGGCGGAACAGCTGCAGCGCACAGAGGCAATCACTCAGGATAATGG
  TGGCTCTCACTTCTCCTGGGCCAAGGAGGCCGAAAAGCGCAATGAACTTTGGGCAGCGCGGCACAATGCT
  TGGTATGCAGCCTTGGCCCTGAGTCCAGGAAGTAAGGCCTATTCCACGGACGTGTGTGTACCCATCTCCC
  GGCTGCCAGAGATCTTGGTAGAGACCAAGGAGGAGATTAAAGCTTCAAAACTCACAGGAGCCATCGTTGG
  GCATGTGGGTGATGGCAACTTTCACTGCATCCTCCTGGTCGACCCAGATGATGCAGAGGAGCAGAGGAGG
  GTCAAGGCCTTTGCAGAAAATCTGGGCAGGCGGGCCCTGGCACTCGGTGGGACATGCACTGGAGAACATG
  GCATCGGACTGGGCAAGCGACAGCTGCTCCAAGAGGAAGTGGGCCCCGTGGGCGTGGAGACCATGCGGCA
  GCTCAAAAACACACTGGATCCCCGTGGTCTCATGAACCCAGGCAAAGTGCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207759 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMLLRVATQRLSPWRSFCSRGSQGGLSQDFVEALKAVVGSPHVSTASAVREQHGHDESMHRCQPPDAVV
  WPQNVDQVSRVASLCYNQGVPIIPFGTGTGVEGGVCAVQGGVCINLTHMDQITELNTEDFSVVVEPGVTR
  KALNTHLRDSGLWFPVDPGADASLCGMAATGASGTNAVRYGTMRDNVINLEVVLPDGRLLHTAGRGRHYR
  KSAAGYNLTGLFVGSEGTLGIITSTTLRLHPAPEATVAATCAFPSVQAAVDSTVQILQAAVPVARIEFLD
  DVMMDACNRHSKLNCPVAPTLFLEFHGSQQTLAEQLQRTEAITQDNGGSHFSWAKEAEKRNELWAARHNA
  WYAALALSPGSKAYSTDVCVPISRLPEILVETKEEIKASKLTGAIVGHVGDGNFHCILLVDPDDAEEQRR
  VKAFAENLGRRALALGGTCTGEHGIGLGKRQLLQEEVGPVGVETMRQLKNTLDPRGLMNPGKVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027570
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027570.1, NM_027570.2, NM_027570.3, NP_081846.3
• RefSeq Size: 2235 bp
• RefSeq ORF: 1455 bp
• Locus ID: 52815
• MW: 51.8 kDa