Cacnb4 (NM_146123) Mouse Tagged ORF Clone

CAT#: MR207780

Cacnb4 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, beta 4 subunit (Cacnb4), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacnb4
• Synonyms: 3110038O15Rik; Cchb4; lh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207780 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGACAATTTGTACCTGCATGGAGTTGAAGACTCGGAGGCTGGGTCAGCAGATTCCTATACAAGCA
  GGCCATCGGACTCCGATGTCTCCTTGGAAGAGGACCGGGAAGCGATCCGGCAAGAGCGAGAACAGCAAGC
  AGCTATCCAGCTTGAGAGAGCGAAGTCCAAACCTGTAGCTTTTGCGGTGAAAACGAACGTGAGCTACTGT
  GGTGCCCTGGACGAAGATGTGCCCGTTCCCAGCACAGCCATCTCCTTTGACGCCAAGGACTTTCTTCACA
  TTAAAGAGAAATATAACAATGATTGGTGGATAGGAAGACTGGTAAAAGAGGGCTGTGAGATTGGCTTCAT
  CCCAAGTCCACTGCGCTTGGAGAATATTCGGATTCAACAGGAACAGAAAAGAGGCCGTTTTCATGGCGGG
  AAATCGAGTGGAAACTCTTCCTCCAGTCTGGGAGAAATGGTATCAGGAACATTCCGAGCAACTCCCACAA
  CAACAGCAAAACAGAAGCAGAAAGTGACGGAGCACATTCCTCCGTATGACGTCGTGCCGTCAATGCGTCC
  TGTGGTGTTAGTGGGGCCATCACTGAAAGGTTATGAGGTAACAGACATGATGCAGAAAGCCCTCTTTGAT
  TTCCTGAAGCACAGGTTTGATGGGAGGATATCAATAACAAGAGTGACAGCTGACATTTCTCTTGCTAAGA
  GATCTGTCCTCAACAATCCTAGCAAGAGAGCAATAATTGAACGTTCCAACACCAGATCCAGCTTAGCGGA
  AGTACAAAGTGAAATTGAAAGAATTTTTGAGTTGGCAAGATCTTTGCAATTGGTTGTTCTTGATGCAGAC
  ACCATCAACCACCCAGCACAGCTGATAAAGACATCCTTAGCACCCATCATCGTCCACGTGAAGGTCTCGT
  CCCCAAAGGTTTTACAGCGGCTGATTAAGTCCAGAGGAAAGTCCCAAAGCAAACACTTGAATGTTCAACT
  GGTGGCGGCCGATAAACTGGCCCAGTGCCCGCCTGAAATGTTTGATGTTATATTAGATGAGAATCAACTT
  GAGGATGCCTGTGAACATCTGGGAGAGTACCTGGAGGCATACTGGCGTGCCACCCACACGAGCAGTAGCA
  CCCCTATGACCCCATTACTGGGGCGGAACGTGGGCTCCACAGCCCTCTCACCATATCCCACAGCAATCTC
  TGGATTACAGAGTCAGCGAATGAGACACAGCAACCATTCTACAGAGAATTCTCCAATTGAAAGACGAAGC
  CTAATGACCTCGGATGAAAATTACCACAATGAGAGGGCCCGCAAGAGTAGGAACCGCTTGTCTTCCAGCT
  CCCAGCACAGCCGAGACCACTACCCTCTGGTGGAAGAAGATTACCCGGACTCGTACCAGGACACTTATAA
  GCCCCATAGGAACCGAGGATCGCCCGGGGGGTGCAGCCATGACTCCCGACATAGGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207780 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYDNLYLHGVEDSEAGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYC
  GALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGG
  KSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFD
  FLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDAD
  TINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQL
  EDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRS
  LMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146123
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146123.1, NM_146123.2, NM_146123.3, NP_666235.1
• RefSeq Size: 7928 bp
• RefSeq ORF: 1461 bp
• Locus ID: 12298
• MW: 54.6 kDa