Nampt (NM_021524) Mouse Tagged ORF Clone

CAT#: MR207867

Nampt (Myc-DDK-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nampt
• Synonyms: 1110035O14Rik; AI314458; AI480535; NAmPRTase; Pbef; Pbef1; Visfatin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207867 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCTGCGGCAGAAGCCGAGTTCAACATCCTGCTGGCCACCGACTCGTACAAGGTTACTCACTATA
  AACAATACCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAATGCCGTGAAAAGAAGACAGAAAACTC
  CAAAGTAAGGAAGGTGAAATACGAGGAAACAGTATTTTATGGGTTGCAGTACATTCTTAATAAGTACTTA
  AAAGGTAAAGTAGTGACCAAAGAGAAAATCCAGGAGGCCAAAGAAGTGTACAGAGAACATTTCCAAGATG
  ATGTCTTTAACGAAAGAGGATGGAACTACATCCTTGAGAAATACGATGGTCATCTCCCAATTGAAGTAAA
  GGCTGTTCCCGAGGGCTCTGTCATCCCCAGAGGGAACGTGCTGTTCACAGTGGAAAACACAGACCCAGAG
  TGCTACTGGCTTACCAATTGGATTGAGACTATTCTTGTTCAGTCCTGGTATCCAATTACAGTGGCCACAA
  ATTCCAGAGAACAGAAGAAAATACTGGCCAAATATTTGTTAGAAACCTCTGGTAACTTAGATGGTCTGGA
  ATACAAGTTACATGACTTTGGTTACAGAGGAGTCTCTTCGCAAGAGACTGCTGGCATAGGGGCATCTGCT
  CATTTGGTTAACTTTAAAGGAACAGATACTGTGGCGGGAATTGCTCTAATTAAAAAATACTATGGGACAA
  AAGATCCTGTTCCAGGCTATTCTGTTCCAGCAGCAGAGCACAGTACCATAACGGCTTGGGGGAAAGACCA
  TGAGAAAGATGCTTTTGAACACATAGTAACACAGTTCTCATCAGTGCCTGTGTCTGTGGTCAGCGATAGC
  TATGACATTTATAATGCGTGTGAGAAAATATGGGGTGAAGACCTGAGACATCTGATAGTATCGAGAAGTA
  CAGAGGCACCACTAATCATCAGACCTGACTCTGGAAATCCTCTTGACACTGTATTGAAGGTCTTAGATAT
  TTTAGGCAAGAAGTTTCCTGTTACTGAGAACTCAAAAGGCTACAAGTTGCTGCCACCTTATCTTAGAGTC
  ATTCAAGGAGATGGCGTGGATATCAATACTTTACAAGAGATTGTAGAGGGAATGAAACAAAAGAAGTGGA
  GTATCGAGAATGTCTCCTTCGGTTCTGGTGGCGCTTTGCTACAGAAGTTAACCCGAGACCTCTTGAATTG
  CTCCTTCAAGTGCAGCTATGTTGTAACCAATGGCCTTGGGGTTAATGTGTTTAAGGACCCAGTTGCTGAT
  CCCAACAAAAGGTCAAAAAAGGGCCGGTTATCTTTACATAGGACACCAGCGGGGAACTTTGTTACACTTG
  AAGAAGGAAAAGGAGACCTTGAGGAATATGGCCATGATCTTCTCCATACGGTTTTCAAGAATGGGAAGGT
  GACAAAAAGCTACTCATTTGATGAAGTCAGAAAAAATGCACAGCTGAACATCGAGCAGGACGTGGCACCT
  CAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207867 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYL
  KGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPE
  CYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASA
  HLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDS
  YDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRV
  IQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVAD
  PNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAP
  H
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021524
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021524.1, NM_021524.2, NP_067499.1
• RefSeq Size: 4547 bp
• RefSeq ORF: 1476 bp
• Locus ID: 59027
• MW: 55.4 kDa