Pltp (NM_011125) Mouse Tagged ORF Clone

CAT#: MR207917

Pltp (Myc-DDK-tagged) - Mouse phospholipid transfer protein (Pltp)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pltp
• Synonyms: Bpife; OD107
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207917 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTGCTCTGGGCCCTCTTCCTAGCGCTCCTGGCAGGTGCCCACGCCGAGCTCCCGGGCTGCAAGA
  TCCGCGTCACCTCCGCGGCTCTGGAGCTGGTGAAGCAGGAAGGCCTGCGCTTTCTGGAACAAGAGCTGGA
  GACCATCACCATCCCAGACGTGTACGGCGCAAAGGGCCACTTTTACTACAATATCTCGGACGTGAGGGTC
  ACACAGCTGCACCTGATATCCTCGGAACTCCACTTCCAGCCAGACCAAGATCTGCTGCTGAACATCTCCA
  ACGCATCCTTGGGGCTGCACTTCCGGAGGCAGCTACTCTACTGGTTCTTATATGATGGGGGCTACATCAA
  TGCCTCGGCGGAGGGTGTGTCCATCCGAACAGGTCTACAGCTCTCCCAGGATTCCAGCGGTCGGATTAAA
  GTGTCCAATGTCTCCTGCGAGGCCTCTGTGTCTAAAATGAATATGGCCTTCGGGGGAACCTTCAGGAGGA
  TGTATAACTTTTTCTCCACGTTCATCACTTCTGGGATGCGGTTCCTCCTCAACCAGCAGATCTGCCCTGT
  GCTCTACCATGCTGGGACGGTGTTGCTCAACTCCCTCCTGGACACGGTGCCCGTGCGCAGTTCTGTGGAT
  GATCTCGTGGGCATCGATTACTCCCTTCTGAAGGATCCTGTGGTCTCCAACGGCAATCTGGATATGGAAT
  TCCGGGGAGCATTCTTCCCTCTGAAGGAGGACAACTGGAGCCTCCCCAACAGGGCAGTGGAGCCTCAGCT
  GGAGGACGACGAGAGGATGGTGTACGTGGCCTTCTCCGAGTTCTTTTTTGACTCCGCCATGGAGAGCTAC
  TTCCAGGCTGGAGCGCTGCAGCTGACTCTTGTTGGGGACAAGGTGCCGAGTGACCTGGACATGCTTCTGA
  GAGCCACCTACTTTGGGAGCATTGTTCTCCTGAGTCCGACAGTGATTAACTCCCCACTGAAGCTGAAGCT
  AGAGGCCACGAGCCCTCCACGTTGTACCATCAAGCCGTCAGGCACCACCATCTCCATCACCGCCAGCGTC
  ACCATCACCTTGGCCCCGCCCATGTTGCCGGAAGTGGAGCTGTCCAAGATGATCATGGAAGGCCGTCTCA
  GTGCTAAGTTGACACTCCGGGGCAAGGCGCTGCGAGTGAAGCTGGACCTTCGAAGGTTTCAAATCTACTC
  AAATCAGTCTGCGCTGGAGTCTCTGGCGCTGATCCCACTGCAGGCCCCACTGAAGACACTGCTGCAAATC
  GGAGTGATGCCTTTGCTGAACGAGCGTACCTGGCGTGGGGTGCAGATCCCCCTTCCTGAGGGTATCAACT
  TCGTGCGTGAGGTGGTGACCAACCACGCGGGCTTCGTCACTGTTGGGGCTGACCTCCACTTTGCCAAAGG
  GCTTCGAGAAGTGATTGACAAGAACCGTCCTGCAGACGTTGCGGCCTCCCATGTCCCCCCACCCTCTGCT
  GCAGCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207917 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLLWALFLALLAGAHAELPGCKIRVTSAALELVKQEGLRFLEQELETITIPDVYGAKGHFYYNISDVRV
  TQLHLISSELHFQPDQDLLLNISNASLGLHFRRQLLYWFLYDGGYINASAEGVSIRTGLQLSQDSSGRIK
  VSNVSCEASVSKMNMAFGGTFRRMYNFFSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVD
  DLVGIDYSLLKDPVVSNGNLDMEFRGAFFPLKEDNWSLPNRAVEPQLEDDERMVYVAFSEFFFDSAMESY
  FQAGALQLTLVGDKVPSDLDMLLRATYFGSIVLLSPTVINSPLKLKLEATSPPRCTIKPSGTTISITASV
  TITLAPPMLPEVELSKMIMEGRLSAKLTLRGKALRVKLDLRRFQIYSNQSALESLALIPLQAPLKTLLQI
  GVMPLLNERTWRGVQIPLPEGINFVREVVTNHAGFVTVGADLHFAKGLREVIDKNRPADVAASHVPPPSA
  AAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011125
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011125.2, NP_035255.1
• RefSeq Size: 1806 bp
• RefSeq ORF: 1482 bp
• Locus ID: 18830
• MW: 54.5 kDa