Chrna6 (NM_021369) Mouse Tagged ORF Clone

CAT#: MR207940

Chrna6 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 6 (Chrna6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna6
• Synonyms: Acra6; Nica6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207940 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACAGCCGGGACCAGGGAAACCTGCACTCCGGTTTATGTCTGTGGCTATGTGGATTCCTGGCTC
  TCTTTAAAGGCAGTACAGGCTGTGAATCTGAAGAGCAGCTCTTCCACAGGCTGTTTGCTCACTACAACCG
  CTTCATCCGGCCGGTGGAGAATGTCTCCGATCCCGTCACGGTGCATTTTGAATTGGCAATCACGCAACTG
  GCCAATGTGGATGAAGTCAACCAGATTATGGAGACCAATCTGTGGCTGCGTCACATCTGGAAGGACTACA
  GATTGCGTTGGGATCCAACGGAGTATGATGGCATCGAGACACTTCGAGTTCCAGCAGACAACATCTGGAA
  GCCTGACATCGTTCTGTATAACAATGCTGTCGGCGACTTCCAGGTCGAAGGCAAGACCAAAGCTCTTCTC
  AAGTATGACGGTGTGATAACCTGGACCCCACCAGCCATCTTTAAGAGCTCCTGCCCAATGGACATCACCT
  TCTTCCCGTTTGACCATCAAAACTGCTCCCTGAAGTTTGGTTCCTGGACTTATGACAAGGCAGAAATCGA
  CCTTCTCATCATTGGCTCTAAAGTAGACATGAACGACTTTTGGGAAAACAGTGAATGGGAAATTGTCGAC
  GCCTCTGGCTATAAGCATGACATCAAGTACAACTGCTGTGAAGAGATTTACACGGACATAACCTACTCCT
  TCTACATCAGGAGGTTGCCCATGTTTTACACCATCAACCTCATCATCCCCTGCCTCTTCATTTCCTTCCT
  CACGGTGCTGGTTTTTTACCTTCCCTCCGACTGTGGCGAGAAAGTGACTCTTTGCATCTCCGTTCTGCTT
  TCTCTCACTGTCTTTTTGCTGGTGATTACAGAGACCATCCCATCCACATCTCTCGTGATCCCACTGGTGG
  GTGAGTATCTACTGTTCACCATGATCTTTGTCACGCTGTCCATTGTGGTGACCGTGTTCGTGCTGAACAT
  ACACTACAGGACCCCAGCAACGCATACCATGCCCAAGTGGGTGAAGACCATCTTCCTTCAGGCCTTCCCC
  TCGATTCTGATGATGAGGAAACCTCTGGACAAGACAAAGGAGGCAGGAGGTGTTAAGGACCCCAAAAGCC
  ATACCAAGAGGCCTGCCAAGGTCAAATTTACTCATCGAGGAGAATCCAAACTTCTAAAGGAATGCCACCA
  CTGCCAAAAATCAAGTGACATAGCACCTGGAAAGAGAAGATCAAGCCAGCAGCCTGCACGGTGGGTGGCA
  GAGAATTCAGAGCACTCGTCCGATGTTGAAGATGTCATCGAGAGTGTTCAATTCATAGCAGAAAACATGA
  AGAGCCACAATGAAACAAACGAGGTAGAAGACGACTGGAAATACATGGCTATGGTGGTGGACAGAGTCTT
  CCTTTGGGTATTTATAATTGTCTGTGTGTTTGGAACTGTGGGGCTATTTCTGCAGCCACTGCTTGGGAAC
  ACAGGAAAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207940 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNSRDQGNLHSGLCLWLCGFLALFKGSTGCESEEQLFHRLFAHYNRFIRPVENVSDPVTVHFELAITQL
  ANVDEVNQIMETNLWLRHIWKDYRLRWDPTEYDGIETLRVPADNIWKPDIVLYNNAVGDFQVEGKTKALL
  KYDGVITWTPPAIFKSSCPMDITFFPFDHQNCSLKFGSWTYDKAEIDLLIIGSKVDMNDFWENSEWEIVD
  ASGYKHDIKYNCCEEIYTDITYSFYIRRLPMFYTINLIIPCLFISFLTVLVFYLPSDCGEKVTLCISVLL
  SLTVFLLVITETIPSTSLVIPLVGEYLLFTMIFVTLSIVVTVFVLNIHYRTPATHTMPKWVKTIFLQAFP
  SILMMRKPLDKTKEAGGVKDPKSHTKRPAKVKFTHRGESKLLKECHHCQKSSDIAPGKRRSSQQPARWVA
  ENSEHSSDVEDVIESVQFIAENMKSHNETNEVEDDWKYMAMVVDRVFLWVFIIVCVFGTVGLFLQPLLGN
  TGKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021369
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021369.1, NM_021369.2, NP_067344.2
• RefSeq Size: 2921 bp
• RefSeq ORF: 1485 bp
• Locus ID: 11440
• MW: 56.9 kDa