Angpt1 (NM_009640) Mouse Tagged ORF Clone

CAT#: MR207984

Angpt1 (Myc-DDK-tagged) - Mouse angiopoietin 1 (Angpt1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angpt1
• Synonyms: 1110046O21Rik; Ang-1; Ang1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207984 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGTTTTCCTTTCCTTTGCATTCTTCGCTGCCATTCTGACTCACATAGGGTGCAGCAACCAGCGCC
  GAAATCCAGAAAACGGAGGGAGAAGATATAACCGGATTCAACATGGGCAATGTGCCTACACTTTCATTCT
  TCCAGAACACGACGGGAACTGCCGTGAGAGTGCGACAGAGCAGTACAACACCAACGCTCTGCAAAGGGAT
  GCTCCACACGTGGAGCCGGATTTCTCTTCCCAGAAACTTCAGCATCTGGAGCATGTGATGGAAAATTATA
  CTCAGTGGCTGCAAAAACTTGAGAATTACATTGTGGAAAATATGAAGTCGGAGATGGCCCAGATACAACA
  GAATGCTGTTCAAAACCACACGGCCACCATGCTTGAGATAGGAACCAGTCTCTTATCTCAGACTGCAGAG
  CAGACCCGAAAGCTGACAGATGTTGAGACCCAGGTACTAAATCAAACATCCCGACTTGAAATACAACTGC
  TAGAGAATTCATTATCAACATACAAGCTAGAGAAGCAACTTCTCCAACAGACAAATGAAATTCTGAAGAT
  TCACGAAAAAAACAGTTTACTAGAGCACAAAATCTTAGAAATGGAGGGAAAACACAAAGAAGAATTGGAC
  ACCTTGAAGGAGGAGAAAGAAAACCTTCAAGGCTTGGTTTCTCGTCAGACATTCATCATCCAGGAGTTGG
  AGAAGCAACTTAGTAGAGCTACCAACAACAACAGCATCCTGCAGAAGCAACAACTGGAGCTCATGGACAC
  AGTTCATAACCTTATCAGCCTTTGCACTAAAGAAGGTGTTTTGCTAAAGGGAGGAAAAAGAGAAGAAGAG
  AAACCATTTCGAGACTGTGCGGATGTATATCAAGCTGGTTTTAATAAAAGTGGAATCTACACTATTTATT
  TTAATAATATGCCAGAACCCAAAAAGGTATTTTGCAATATGGATGTGAATGGGGGAGGTTGGACAGTAAT
  ACAACACCGGGAAGATGGAAGCCTGGATTTCCAGAGGGGCTGGAAGGAGTATAAAATGGGTTTTGGGAAT
  CCCTCTGGTGAATATTGGCTTGGGAACGAGTTCATTTTTGCAATAACCAGTCAGAGGCAGTACATGCTGA
  GGATTGAGCTGATGGACTGGGAAGGGAACCGAGCCTACTCACAGTACGACAGATTCCACATAGGAAATGA
  AAAGCAGAACTATAGGTTATATTTAAAAGGTCACACAGGGACAGCAGGCAAACAGAGCAGCTTGATCTTA
  CACGGTGCCGATTTCAGCACGAAGGATGCTGATAACGACAACTGTATGTGCAAATGCGCTCTCATGCTAA
  CAGGAGGTTGGTGGTTCGATGCCTGTGGCCCTTCCAATCTAAATGGAATGTTCTACACTGCGGGACAAAA
  TCATGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAAGGGCCCAGTTACTCCTTACGTTCCACCACC
  ATGATGATCCGGCCCTTGGACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207984 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVFLSFAFFAAILTHIGCSNQRRNPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRD
  APHVEPDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAE
  QTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELD
  TLKEEKENLQGLVSRQTFIIQELEKQLSRATNNNSILQKQQLELMDTVHNLISLCTKEGVLLKGGKREEE
  KPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGN
  PSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLIL
  HGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTT
  MMIRPLDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009640
• ORF Size: 1497 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009640.1, NM_009640.2, NM_009640.3, NM_009640.4, NP_033770.2
• RefSeq Size: 4316 bp
• RefSeq ORF: 1497 bp
• Locus ID: 11600
• MW: 57.5 kDa
• Gene Summary: This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]