Lipg (NM_010720) Mouse Tagged ORF Clone

CAT#: MR208018

Lipg (Myc-DDK-tagged) - Mouse lipase, endothelial (Lipg)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipg
• Synonyms: 3110013K01Rik; EL; lipase; mEDL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208018 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAAACACGGTTTTCCTGCTCGGCTTTTGGAGCGTCTATTGTTACTTCCCGGCGGGAAGTATCACAA
  CCTTGCGTCCCGAGGGGTCGCTGCGAGATGAGCATCATAAACCCACTGGAGTACCAGCTACCGCCAGACC
  CTCTGTGGCTTTTAACATCCGCACTTCTAAGGACCCAGAGCAGGAAGGGTGTAATCTCTCCCTTGGTGAC
  AGCAAACTCTTAGAAAACTGTGGCTTCAACATGACAGCCAAAACCTTCTTCATCATTCATGGATGGACGA
  TGAGTGGCATGTTTGAGAGCTGGCTGCATAAACTTGTATCAGCCCTGCAGATGAGAGAGAAAGATGCTAA
  CGTCGTGGTGGTTGACTGGCTGCCCCTGGCTCATCAGCTGTACACGGATGCAGTCAATAACACCAGGGTG
  GTGGGACAGAGAGTAGCTGGGATGCTTGACTGGCTGCAGGAGAAGGAAGAGTTCTCTCTTGGGAACGTTC
  ACTTGATTGGCTACAGCCTTGGAGCACACGTGGCTGGATACGCTGGCAACTTTGTGAAAGGAACAGTGGG
  CAGGATCACTGGTCTGGATCCCGCGGGTCCCATGTTTGAAGGGGTGGACATCAACAGAAGGCTGTCCCCG
  GACGATGCAGACTTTGTGGATGTCCTGCATACCTACACGCTGTCCTTTGGCTTGAGCATTGGGATTCGGA
  TGCCTGTGGGTCACATTGACATCTATCCCAATGGCGGTGACTTCCAGCCAGGCTGTGGATTCAATGATGT
  CATCGGATCTTTTGCATATGGAACAATCTCAGAGATGGTGAAATGCGAGCACGAGCGAGCCGTACACCTC
  TTTGTCGACTCTCTGGTGAATCAGGACAAGCCCAGCTTTGCCTTCCAGTGCACAGACTCCAGCCGCTTCA
  AAAGGGGAATCTGCCTCAGCTGCCGGAAGAACCGTTGTAATAACATTGGCTACAACGCCAAGAAAATGAG
  AAAGAAGAGGAATAGCAAAATGTATTTAAAAACCCGGGCTGGCATGCCTTTCAAAGTTTACCATTACCAG
  CTGAAAGTTCACATGTTCTCTTACAATAACAGTGGGGACACCCAGCCCACCCTCTACATTACCCTGTATG
  GTAGCAACGCAGACTCCCAGAACCTGCCCTTGGAAATAGTGGAGAAGATTGAGCTGAATGCCACAAACAC
  CTTCCTTGTCTACACTGAGGAGGACTTGGGCGATCTCTTGAAGATGCGACTTACCTGGGAGGGGGTAGCC
  CATTCCTGGTACAACCTGTGGAATGAGTTTCGCAACTACCTGTCTCAACCCAGCAACCCCTCGAGGGAGC
  TGTACATCCGGCGAATTCGTGTCAAATCTGGGGAAACCCAGCGCAAAGTGACATTTTGCACTCAAGACCC
  AACGAAGAGTAGCATCTCCCCTGGCCAGGAGCTGTGGTTTCACAAGTGTCAGGATGGCTGGAAAATGAAA
  AACAAAACCAGTCCCTTTGTGAACTTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208018 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRNTVFLLGFWSVYCYFPAGSITTLRPEGSLRDEHHKPTGVPATARPSVAFNIRTSKDPEQEGCNLSLGD
  SKLLENCGFNMTAKTFFIIHGWTMSGMFESWLHKLVSALQMREKDANVVVVDWLPLAHQLYTDAVNNTRV
  VGQRVAGMLDWLQEKEEFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGVDINRRLSP
  DDADFVDVLHTYTLSFGLSIGIRMPVGHIDIYPNGGDFQPGCGFNDVIGSFAYGTISEMVKCEHERAVHL
  FVDSLVNQDKPSFAFQCTDSSRFKRGICLSCRKNRCNNIGYNAKKMRKKRNSKMYLKTRAGMPFKVYHYQ
  LKVHMFSYNNSGDTQPTLYITLYGSNADSQNLPLEIVEKIELNATNTFLVYTEEDLGDLLKMRLTWEGVA
  HSWYNLWNEFRNYLSQPSNPSRELYIRRIRVKSGETQRKVTFCTQDPTKSSISPGQELWFHKCQDGWKMK
  NKTSPFVNLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010720
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010720.1, NM_010720.2, NM_010720.3, NP_034850.2
• RefSeq Size: 3787 bp
• RefSeq ORF: 1503 bp
• Locus ID: 16891
• MW: 56.6 kDa