Dars (NM_177445) Mouse Tagged ORF Clone

CAT#: MR208055

Dars (Myc-DDK-tagged) - Mouse aspartyl-tRNA synthetase (Dars), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dars
• Synonyms: 5730439G15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208055 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCACCAACGCCAGCCGTAAGGGTCAGGAGAAGCCGCGGGAGATCGTGGACGCGGCGGAAGATT
  ATGCTAAAGAGAGATATGGGATATCTTCTATGATACAATCACAAGAAAAGCCAGATAGAGTTTTGGTTCG
  AGTTAAGGACCTGACAGTTCAAAAAGCTGATGATGTTGTTTGGGTCCGTGCAAGAGTTCATACAAGCAGA
  GCAAAAGGGAAGCAGTGCTTCTTAGTCCTGAGGCAGCAGCAGTTTAATGTCCAGGCTCTTGTGGCGGTGG
  GAGACCATGCAAGCAAGCAGATGGTTAAATTTGCTGCCAACATCAACAAAGAAAGTATTATTGACGTAGA
  AGGTGTTGTGAGGAAAGTCAATCAGAAAATTGGAAGCTGTACCCAGCAGGATGTTGAGTTGCATGTCCAG
  AAGATTTATGTGATCAGCTTGGCTGAACCTCGCCTGCCCTTGCAGCTGGATGATGCCATTCGGCCTGAAG
  TGGAAGGAGAAGAGGACGGAAGAGCTACGGTTAACCAGGATACAAGATTAGACAACAGAGTCATTGACCT
  TAGGACATCAACTAGTCAGGCCATCTTCCGTCTCCAGTCCGGCATCTGCCATCTCTTCCGAGAAACTTTA
  ATTAACAAAGGTTTTGTGGAAATCCAAACTCCTAAAATAATTTCAGCTGCCAGTGAAGGAGGAGCCAATG
  TTTTTACTGTGTCCTATTTTAAAAATAATGCCTACCTTGCTCAGTCTCCACAATTGTATAAGCAGATGTG
  CATTTGTGCTGATTTTGAGAAGGTTTTCTGCATTGGACCAGTTTTCAGAGCTGAAGACTCCAATACCCAT
  AGACATCTGACTGAATTTGTTGGTTTGGATATTGAAATGGCCTTTAATTATCATTACCATGAAGTTGTAG
  AAGAGATTGCTGACACTTTGGTGCAAATATTCAAAGGACTTCAAGAAAGGTTTCAGACTGAAATTCAAAC
  TGTGAGTAAACAGTTCCCATGTGAGCCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAG
  GCTTTGGCAATGCTTAGAGAAGCTGGAGTTGAAATGGACGATGAGGAAGATCTAAGTACACCAAATGAAA
  AACTGTTGGGTCGTTTGGTGAAGGAAAAGTATGATACAGACTTTTATGTTCTTGATAAATATCCATTGGC
  TGTAAGACCTTTCTATACCATGCCTGACCCAAGAAATCCGAAACAGTCCAACTCTTACGATATGTTCATG
  AGAGGAGAAGAAATATTGTCTGGAGCACAAAGAATCCATGATCCTCAGTTGCTTACAGAGAGAGCTTTAC
  ACCATGGAATTGATTTGGAGAAAATCAAAGCTTACATTGATTCCTTCCGCTTTGGAGCTCCTCCTCATGC
  TGGTGGTGGCATTGGGCTGGAAAGGGTGACGATGCTGTTTTTAGGATTGCATAACGTACGCCAGACCTCA
  ATGTTCCCTCGTGACCCCAAACGACTCACGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208055 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSTNASRKGQEKPREIVDAAEDYAKERYGISSMIQSQEKPDRVLVRVKDLTVQKADDVVWVRARVHTSR
  AKGKQCFLVLRQQQFNVQALVAVGDHASKQMVKFAANINKESIIDVEGVVRKVNQKIGSCTQQDVELHVQ
  KIYVISLAEPRLPLQLDDAIRPEVEGEEDGRATVNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETL
  INKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTH
  RHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCE
  ALAMLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQSNSYDMFM
  RGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTS
  MFPRDPKRLTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177445
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177445.1, NM_177445.2, NM_177445.3, NM_177445.4, NM_177445.5, NP_803228.2
• RefSeq Size: 2249
• RefSeq ORF: 1506
• Locus ID: 226414
• MW: 57.1 kDa
• Gene Summary: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. [UniProtKB/Swiss-Prot Function]