Fto (NM_011936) Mouse Tagged ORF Clone

CAT#: MR208064

Fto (Myc-DDK-tagged) - Mouse fat mass and obesity associated (Fto)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fto
• Synonyms: AW743446; mKIAA1752
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208064 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCGTCCAGACCGCGGAGGAACGAGAGCGGGAAGCTAAGAAACTGAGGCTCCTTGAGGAGCTTG
  AAGACACTTGGCTTCCTTACCTGACCCCCAAAGATGATGAGTTCTATCAGCAGTGGCAGCTGAAATACCC
  TAAACTGGTTTTCCGAGAGGCCGGCAGCATACCAGAGGAGCTGCATAAGGAGGTCCCCGAGGCCTTTCTC
  ACACTGCATAAGCATGGCTGCTTGTTTCGGGACGTGGTGAGGATCCAAGGCAAAGATGTGCTCACCCCAG
  TGTCTCGCATCCTCATCGGGGACCCAGGCTGCACCTACAAGTACTTGAACACCAGACTCTTCACGGTGCC
  CTGGCCCGTGAAGGGCTGCACGGTCAAGTACACAGAGGCTGAGATCGCCGCTGCATGTCAGACCTTCCTA
  AAGCTCAATGACTACCTCCAGGTGGAGACCATCCAGGCCTTGGAAGAACTGGCTGTCAGAGAGAAGGCCA
  ATGAAGACGCTGTGCCACTGTGCATGGCAGAGTTCCCCAGGGCCGGCGTGGGGCCGTCCTGCGATGATGA
  AGTGGACCTTAAGAGCAGAGCAGCCTACAACGTGACTTTGCTAAACTTCATGGATCCTCAGAAGATGCCC
  TACTTGAAAGAGGAGCCCTATTTCGGCATGGGGAAGATGGCGGTGAGCTGGCATCACGATGAGAACCTGG
  TGGACAGGTCAGCCGTGGCAGTGTACAGCTATAGCTGCGAAGGCTCTGAGGATGAAAGTGAGGACGAGTC
  CAGCTTCGAAGGCAGAGATCCTGATACTTGGCATGTTGGTTTTAAGATCTCTTGGGACATCGAGACACCA
  GGATTAACAATCCCTCTTCACCAGGGAGACTGCTATTTCATGCTGGATGACCTCAATGCCACCCACCAGC
  ACTGTGTTTTGGCTGGCTCACAGCCTCGGTTTAGTTCCACTCACCGTGTGGCAGAGTGCTCAACAGGCAC
  CTTGGATTATATCTTAGAACGCTGTCAGTTGGCGCTGCAGAATGTCCTCAATGACTCAGACGATGGCGAC
  GTCTCGTTGAAATCCTTTGATCCTGCAGTTTTGAAACAAGGAGAGGAAATCCATAATGAGGTGGAGTTTG
  AGTGGCTGAGGCAGTTCTGGTTTCAAGGCAATCGATACAAACTTTGCACCGATTGGTGGTGTGAGCCCAT
  GACTCACCTGGAGGGGCTGTGGAAGAAGATGGAGAGCATGACAAATGCGGTGCTCCGTGAAGTTAAAAGA
  GAGGGGCTCCCGGTGGAACAAAGGAGTGAGATTCTGTCTGCCATCCTGGTCCCGCTCACCGTGCGCCAGA
  ACCTGAGGAAGGAGTGGCATGCCAGGTGCCAGTCCCGAGTCGTCCGGACTTTACCAGTACAGCAGAAACC
  AGACTGCCGGCCATATTGGGAGAAGGATGACCCTTCCATGCCTCTGCCCTTTGACCTCACAGACGTGGTT
  TCCGAGCTCAGAGGCCAGCTGCTGGAAGCAAGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208064 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRVQTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLVFREAGSIPEELHKEVPEAFL
  TLHKHGCLFRDVVRIQGKDVLTPVSRILIGDPGCTYKYLNTRLFTVPWPVKGCTVKYTEAEIAAACQTFL
  KLNDYLQVETIQALEELAVREKANEDAVPLCMAEFPRAGVGPSCDDEVDLKSRAAYNVTLLNFMDPQKMP
  YLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEGSEDESEDESSFEGRDPDTWHVGFKISWDIETP
  GLTIPLHQGDCYFMLDDLNATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILERCQLALQNVLNDSDDGD
  VSLKSFDPAVLKQGEEIHNEVEFEWLRQFWFQGNRYKLCTDWWCEPMTHLEGLWKKMESMTNAVLREVKR
  EGLPVEQRSEILSAILVPLTVRQNLRKEWHARCQSRVVRTLPVQQKPDCRPYWEKDDPSMPLPFDLTDVV
  SELRGQLLEARS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011936
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011936.1, NM_011936.2, NP_036066.1
• RefSeq Size: 3586 bp
• RefSeq ORF: 1509 bp
• Locus ID: 26383
• MW: 58 kDa