Pdia3 (NM_007952) Mouse Tagged ORF Clone

CAT#: MR208116

  • TrueORF®

Pdia3 (Myc-DDK-tagged) - Mouse protein disulfide isomerase associated 3 (Pdia3)


  "NM_007952" in other vectors (4)


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Reconstitution Protocol

USD 470.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Pdia3
Synonyms 58kDa; Erp; ERp57; ERp60; ERp61; Grp58; PDI; PDI-Q2; PI-PLC; Plca; PLC[a]
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208116 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTTCAGCTGCCTAGCTCTGCTCCCGGGCGTGGCGCTGCTGCTCGCCTCGGCCCGTCTCGCCGCCG
CCTCCGATGTGTTGGAACTGACGGACGAAAACTTCGAGAGTCGCGTCTCCGACACGGGCTCGGCGGGGCT
CATGCTGGTCGAGTTCTTCGCCCCCTGGTGTGGACATTGCAAGAGGCTTGCCCCTGAGTATGAAGCTGCA
GCAACCAGATTAAAAGGAATAGTCCCATTAGCAAAGGTGGATTGCACTGCCAACACAAACACCTGTAATA
AGTATGGGGTCAGTGGCTACCCAACTCTTAAGATCTTTAGAGATGGTGAAGAAGCGGGTGCTTATGATGG
GCCTAGGACTGCTGATGGAATTGTCAGCCACTTGAAGAAACAAGCAGGACCAGCTTCAGTTCCTCTCAGG
ACTGAGGAAGAATTTAAGAAGTTCATTAGTGATAAAGATGCCTCAGTGGTGGGTTTTTTCAGGGATTTAT
TCAGTGATGGGCACTCTGAATTCCTAAAAGCAGCCAGCAACTTGAGAGATAACTACCGATTTGCACACAC
CAACATTGAGTCTCTGGTGAAGGAGTACGATGATAATGGAGAGGGGATCACTATATTCCGTCCATTACAT
CTTGCTAACAAGTTTGAAGACAAAACTGTGGCATATACTGAAAAGAAAATGACCAGTGGCAAGATCAAGA
AATTTATTCAGGATAGCATTTTTGGTCTCTGTCCTCATATGACGGAAGATAATAAAGATTTGATACAAGG
CAAGGACTTACTCACCGCTTACTATGATGTGGACTATGAAAAGAATGCTAAAGGTTCTAACTACTGGAGA
AACAGGGTCATGATGGTGGCAAAGAAATTCCTTGATGCTGGACACAAACTCAACTTTGCTGTAGCTAGCC
GTAAAACCTTTAGCCATGAACTGTCAGATTTTGGCTTAGAAAGCACTACTGGAGAGGTTCCTGTTGTGGC
TATCAGAACTGCTAAAGGAGAGAAGTTTGTCATGCAGGAGGAGTTCTCGCGAGATGGCAAGGCTCTTGAA
CAGTTCCTGCAGGAATACTTTGATGGCAACTTGAAGAGATACCTGAAGTCTGAACCCATCCCAGAGTCCA
ACGAAGGGCCTGTCAAGGTTGTGGTAGCAGAGAATTTTGATGACATAGTGAATGAAGAAGATAAGGACGT
GCTGATTGAATTTTACGCCCCTTGGTGTGGCCACTGTAAGAATCTGGAACCCAAGTATAAAGAGCTGGGA
GAAAAACTCAGCAAAGATCCAAATATTGTCATAGCCAAGATGGATGCCACAGCCAATGATGTGCCTTCTC
CATATGAAGTCAAGGGTTTTCCTACCATCTACTTCTCACCAGCCAACAAGAAGCTAACTCCAAAGAAGTA
TGAAGGTGGCCGTGAATTAAATGATTTTATTAGCTATCTACAACGAGAAGCTACAAACCCCCCTATAATT
CAAGAAGAAAAACCTAAGAAGAAGAAGAAGGCACAAGAGGACCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208116 protein sequence
Red=Cloning site Green=Tags(s)

MRFSCLALLPGVALLLASARLAAASDVLELTDENFESRVSDTGSAGLMLVEFFAPWCGHCKRLAPEYEAA
ATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLR
TEEEFKKFISDKDASVVGFFRDLFSDGHSEFLKAASNLRDNYRFAHTNIESLVKEYDDNGEGITIFRPLH
LANKFEDKTVAYTEKKMTSGKIKKFIQDSIFGLCPHMTEDNKDLIQGKDLLTAYYDVDYEKNAKGSNYWR
NRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTTGEVPVVAIRTAKGEKFVMQEEFSRDGKALE
QFLQEYFDGNLKRYLKSEPIPESNEGPVKVVVAENFDDIVNEEDKDVLIEFYAPWCGHCKNLEPKYKELG
EKLSKDPNIVIAKMDATANDVPSPYEVKGFPTIYFSPANKKLTPKKYEGGRELNDFISYLQREATNPPII
QEEKPKKKKKAQEDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007952
ORF Size 1518 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007952.1, NM_007952.2, NP_031978.2
RefSeq Size 2658 bp
RefSeq ORF 1518 bp
Locus ID 14827
MW 56.7 kDa

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