Rtcb (NM_145422) Mouse Tagged ORF Clone

CAT#: MR208125

D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtcb
• Synonyms: AI255213; AI463255; D10Wsu52e; FAAP; HSPC117
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208125 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCGTAACTACAACGATGAGCTACAGTTCTTGGACAAGATCAATAAAAACTGCTGGAGGATCAAGA
  AGGGCTTTGTGCCCAACATGCAGGTTGAAGGAGTGTTTTATGTGAATGATGCTCTGGAAAAACTAATGTT
  TGAGGAATTAAGGAACGCCTGTCGAGGTGGTGGTGTTGGTGGCTTTCTGCCAGCCATGAAGCAGATTGGC
  AATGTGGCAGCCCTGCCTGGAATAGTTCATCGGTCTATCGGGCTTCCTGATGTCCATTCAGGCTATGGGT
  TTGCCATAGGGAACATGGCTGCCTTTGATATGAATGACCCTGAGGCCGTTGTATCCCCAGGTGGTGTCGG
  ATTTGATATTAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAGAGCGATGTACAGCCTGTGAAG
  GAACAACTTGCCCAAGCTATGTTTGACCACATCCCTGTTGGGGTGGGATCAAAAGGTGTCATTCCAATGA
  ATGCCAAAGACTTGGAGGAGGCATTGGAGATGGGGGTGGACTGGTCCCTGAGGGAAGGCTATGCCTGGGC
  TGAAGACAAGGAGCACTGTGAGGAGTATGGAAGGATGCTGCAAGCCGACCCCAATAAGGTCTCACCCAGG
  GCAAAGAAAAGGGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATTATGCAGAAATCCAGGTTG
  TAGATGAGATTTTCAATGAGTATGCCGCCAAGAAGATGGGCATCGACCATAAGGGACAGGTGTGTGTGAT
  GATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCTACAGATGCACTGGTAGCTATGGAAAAGGCC
  ATGAAGAGAGACAAGATTATAGTCAATGACCGGCAGTTGGCTTGTGCTCGGATTGCATCCCCAGAGGGAC
  AAGACTATCTAAAGGGAATGGCTGCAGCTGGAAACTACGCCTGGGTTAACCGCTCCTCTATGACCTTCTT
  AACCCGTCAGGCTTTTGCCAAAGTCTTCAACACAACCCCTGATGACCTGGACCTGCATGTGATCTATGAT
  GTGTCGCACAATATCGCCAAAGTGGAGCAGCACGTGGTGGATGGGAAGGAACGGACGCTGCTGGTGCACA
  GGAAGGGATCCACCCGTGCTTTCCCGCCTCACCACCCCCTCATTGCTGTGGATTATCAACTCACAGGACA
  ACCAGTGCTTATTGGTGGCACCATGGGGACCTGTAGTTACGTTCTGACTGGCACTGAACAAGGCATGACT
  GAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCTTTGTCCAGAGCAAAATCACGTCGTAACTTAG
  ATTTCCAAGATGTCTTAGACAAACTGGCAGACATGGGAATTGCAATCCGGGTTGCTTCCCCCAAGCTGGT
  TATGGAAGAGGCACCAGAGTCCTATAAGAATGTGACAGACGTCGTGAACACCTGCCATGATGCTGGGATC
  AGCAAGAAGGCCATTAAACTGAGACCAATTGCTGTTATTAAAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208125 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRNYNDELQFLDKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIG
  NVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVK
  EQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSPR
  AKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKA
  MKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYD
  VSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMT
  ETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGI
  SKKAIKLRPIAVIKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145422
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145422.1, NM_145422.2, NM_145422.3, NM_145422.4, NP_663397.1
• RefSeq Size: 1979 bp
• RefSeq ORF: 1518 bp
• Locus ID: 28088
• MW: 55.2 kDa