Rtcb (NM_145422) Mouse Tagged ORF Clone
CAT#: MR208125
- TrueORF®
D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e)
"NM_145422" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Rtcb |
Synonyms | AI255213; AI463255; D10Wsu52e; FAAP; HSPC117 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208125 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGTAACTACAACGATGAGCTACAGTTCTTGGACAAGATCAATAAAAACTGCTGGAGGATCAAGA AGGGCTTTGTGCCCAACATGCAGGTTGAAGGAGTGTTTTATGTGAATGATGCTCTGGAAAAACTAATGTT TGAGGAATTAAGGAACGCCTGTCGAGGTGGTGGTGTTGGTGGCTTTCTGCCAGCCATGAAGCAGATTGGC AATGTGGCAGCCCTGCCTGGAATAGTTCATCGGTCTATCGGGCTTCCTGATGTCCATTCAGGCTATGGGT TTGCCATAGGGAACATGGCTGCCTTTGATATGAATGACCCTGAGGCCGTTGTATCCCCAGGTGGTGTCGG ATTTGATATTAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAGAGCGATGTACAGCCTGTGAAG GAACAACTTGCCCAAGCTATGTTTGACCACATCCCTGTTGGGGTGGGATCAAAAGGTGTCATTCCAATGA ATGCCAAAGACTTGGAGGAGGCATTGGAGATGGGGGTGGACTGGTCCCTGAGGGAAGGCTATGCCTGGGC TGAAGACAAGGAGCACTGTGAGGAGTATGGAAGGATGCTGCAAGCCGACCCCAATAAGGTCTCACCCAGG GCAAAGAAAAGGGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATTATGCAGAAATCCAGGTTG TAGATGAGATTTTCAATGAGTATGCCGCCAAGAAGATGGGCATCGACCATAAGGGACAGGTGTGTGTGAT GATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCTACAGATGCACTGGTAGCTATGGAAAAGGCC ATGAAGAGAGACAAGATTATAGTCAATGACCGGCAGTTGGCTTGTGCTCGGATTGCATCCCCAGAGGGAC AAGACTATCTAAAGGGAATGGCTGCAGCTGGAAACTACGCCTGGGTTAACCGCTCCTCTATGACCTTCTT AACCCGTCAGGCTTTTGCCAAAGTCTTCAACACAACCCCTGATGACCTGGACCTGCATGTGATCTATGAT GTGTCGCACAATATCGCCAAAGTGGAGCAGCACGTGGTGGATGGGAAGGAACGGACGCTGCTGGTGCACA GGAAGGGATCCACCCGTGCTTTCCCGCCTCACCACCCCCTCATTGCTGTGGATTATCAACTCACAGGACA ACCAGTGCTTATTGGTGGCACCATGGGGACCTGTAGTTACGTTCTGACTGGCACTGAACAAGGCATGACT GAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCTTTGTCCAGAGCAAAATCACGTCGTAACTTAG ATTTCCAAGATGTCTTAGACAAACTGGCAGACATGGGAATTGCAATCCGGGTTGCTTCCCCCAAGCTGGT TATGGAAGAGGCACCAGAGTCCTATAAGAATGTGACAGACGTCGTGAACACCTGCCATGATGCTGGGATC AGCAAGAAGGCCATTAAACTGAGACCAATTGCTGTTATTAAAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208125 protein sequence
Red=Cloning site Green=Tags(s) MSRNYNDELQFLDKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIG NVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVK EQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSPR AKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKA MKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYD VSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMT ETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGI SKKAIKLRPIAVIKG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145422 |
ORF Size | 1518 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145422.1, NM_145422.2, NM_145422.3, NM_145422.4, NP_663397.1 |
RefSeq Size | 1979 bp |
RefSeq ORF | 1518 bp |
Locus ID | 28088 |
MW | 55.2 kDa |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205587 | D10Wsu52e (untagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e), (10ug) |
USD 210.00 |
|
MG208125 | D10Wsu52e (GFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
USD 520.00 |
|
MR208125L3 | Lenti ORF clone of D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
USD 670.00 |
|
MR208125L4 | Lenti ORF clone of D10Wsu52e (mGFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
USD 670.00 |
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