Pparg (NM_011146) Mouse Tagged ORF Clone

CAT#: MR208132

Pparg (Myc-DDK-tagged) - Mouse peroxisome proliferator activated receptor gamma (Pparg), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pparg
• Synonyms: Nr1c3; PPAR-gamma; PPAR-gamma2; PPARgamma; PPARgamma2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208132 representing NM_011146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAAACTCTGGGAGATTCTCCTGTTGACCCAGAGCATGGTGCCTTCGCTGATGCACTGCCTATGA
  GCACTTCACAAGAAATTACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAACTTCGGAATCAGCTC
  TGTGGACCTCTCCGTGATGGAAGACCACTCGCATTCCTTTGACATCAAGCCCTTTACCACAGTTGATTTC
  TCCAGCATTTCTGCTCCACACTATGAAGACATTCCATTCACAAGAGCTGACCCAATGGTTGCTGATTACA
  AATATGACCTGAAGCTCCAAGAATACCAAAGTGCGATCAAAGTAGAACCTGCATCTCCACCTTATTATTC
  TGAAAAGACCCAGCTCTACAACAGGCCTCATGAAGAACCTTCTAACTCCCTCATGGCCATTGAGTGCCGA
  GTCTGTGGGGATAAAGCATCAGGCTTCCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTTTTCC
  GAAGAACCATCCGATTGAAGCTTATTTATGATAGGTGTGATCTTAACTGCCGGATCCACAAAAAAAGTAG
  AAATAAATGTCAGTACTGTCGGTTTCAGAAGTGCCTTGCTGTGGGGATGTCTCACAATGCCATCAGGTTT
  GGGCGGATGCCACAGGCCGAGAAGGAGAAGCTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAACC
  CAGAGTCTGCTGATCTGCGAGCCCTGGCAAAGCATTTGTATGACTCATACATAAAGTCCTTCCCGCTGAC
  CAAAGCCAAGGCGAGGGCGATCTTGACAGGAAAGACAACGGACAAATCACCATTTGTCATCTACGACATG
  AATTCCTTAATGATGGGAGAAGATAAAATCAAGTTCAAACATATCACCCCCCTGCAGGAGCAGAGCAAAG
  AGGTGGCCATCCGAATTTTTCAAGGGTGCCAGTTTCGATCCGTAGAAGCCGTGCAAGAGATCACAGAGTA
  TGCCAAAAATATCCCTGGTTTCATTAACCTTGATTTGAATGACCAAGTGACTCTGCTCAAGTATGGTGTC
  CATGAGATCATCTACACGATGCTGGCCTCCCTGATGAATAAAGATGGAGTCCTCATCTCAGAGGGCCAAG
  GATTCATGACCAGGGAGTTCCTCAAAAGCCTGCGGAAGCCCTTTGGTGACTTTATGGAGCCTAAGTTTGA
  GTTTGCTGTGAAGTTCAATGCACTGGAATTAGATGACAGTGACTTGGCTATATTTATAGCTGTCATTATT
  CTCAGTGGAGACCGCCCAGGCTTGCTGAACGTGAAGCCCATCGAGGACATCCAAGACAACCTGCTGCAGG
  CCCTGGAACTGCAGCTCAAGCTGAATCACCCAGAGTCCTCTCAGCTGTTCGCCAAGGTGCTCCAGAAGAT
  GACAGACCTCAGGCAGATCGTCACAGAGCACGTGCAGCTACTGCATGTGATCAAGAAGACAGAGACAGAC
  ATGAGCCTTCACCCCCTGCTCCAGGAGATCTACAAGGACTTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208132 representing NM_011146
  Red=Cloning site Green=Tags(s)
  
  MGETLGDSPVDPEHGAFADALPMSTSQEITMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDF
  SSISAPHYEDIPFTRADPMVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNRPHEEPSNSLMAIECR
  VCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRF
  GRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDM
  NSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKNIPGFINLDLNDQVTLLKYGV
  HEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVII
  LSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKVLQKMTDLRQIVTEHVQLLHVIKKTETD
  MSLHPLLQEIYKDLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011146
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011146.1, NM_011146.2, NM_011146.3, NP_035276.1
• RefSeq Size: 1780
• RefSeq ORF: 1518
• Locus ID: 19016
• MW: 58 kDa
• Gene Summary: This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]