Hars2 (NM_080636) Mouse Tagged ORF Clone

CAT#: MR208134

Hars2 (Myc-DDK-tagged) - Mouse histidyl-tRNA synthetase 2, mitochondrial (putative) (Hars2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hars2
• Synonyms: 4631412B19Rik; Harsl; HARSR; HO3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208134 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACCTGGGGCCACTGCGTCGCAGGGCCTGGGCTGCGCTGCTCGGCCAGCTCCTGCGACCTCCCA
  GCACTGTGTGCACCCGCGGCTGTCATAGCCAGGTTGCAAAGGCAGTGTTAACGTCTGAACAACTGAAATC
  CCATCAAGAGAAGCCGAATTTTGTCATCAAGGTTCCAAAGGGCACCAGGGATTTAAGTCCTCAACAGATG
  GTTGTGAGGGAGAAAATCCTCGATAAGATTATCAGCTGTTTCAAACGTCATGGGGCGAAGGGGTTGGATA
  CACCAGCGTTTGAGCTAAAAGAAATGCTCACCGAGAAGTATGAAGACAACTTCGGCCTCATGTATGATTT
  GAAGGATCAAGGTGGAGAGTTGTTGTCTCTGCGCTATGACCTTACTGTCCCTTTTGCTCGATATCTGGCC
  ATGAATAAACTGAAGAAAATGAAACGATACCAAGTTGGAAAGGTCTGGAGGCGAGAGAGCCCAGCTATAG
  CCCAGGGCCGCTACCGGGAGTTCTGCCAGTGTGATTTTGACATTGCTGGTGAGTTTGACCCAATGATCCC
  TGATGCAGAGTGTTTGAGGATCATGTGTGAAATCCTAAGTGGACTGCAGCTGGGGGACTTTCTCATTAAG
  GTAAATGACCGTCGGGTTGTAGATGGGATATTTGCTGTCTGTGGTGTTCCTGAGAGCAAGCTCCGAACCA
  TCTGCTCCTCAATGGACAAACTAGACAAGATGTCTTGGGAAGGCGTGAGGCATGAGATGGTGGCAAAGAA
  AGGTCTAGCTCCCGAGGTGGCCGATCGAATTGGGGACTTCGTCCAATATCATGGGGGGATATCCCTGGTT
  GAGGACTTGTTCAAGGATCCCAGACTATCCCAAAGCCAGCTGGCCTTGCAGGGCCTAGGGGACCTGAAGC
  TGCTCTTTGAATACCTGAGGTTATTTGGAATTGCTGATAAGATCTCCCTTGATCTGAGTCTGGCCCGGGG
  CCTGGACTATTATACTGGAGTGATTTATGAAGCAGTACTGCTAGAGTCTCCAGCTCAGGCTGGAAAGGAA
  ACTCTGAGTGTGGGTAGTGTGGCTGCTGGTGGGCGCTATGACAATCTGGTAGCCCAGTTTGATCCCAAGG
  GCCATCACGTGCCTTGTGTGGGGTTGAGCATTGGAGTAGAGAGAATCTTCTACCTTGTGGAACAGAAGAT
  GAAGATGTCTGGTGAGAAGGTTCGCACCACAGAGACCCAAGTGTTTGTGGCCACACCCCAGAAGAACTTT
  CTCCAAGAACGTTTGAAGATAATTGCAGAGCTTTGGGATGCCGGGATCAAGGCAGAGATGCTTTATAAAA
  ACAACCCTAAACTGTTAACCCAGCTGCACTATTGTGAGAAAGCAGACATTCCTCTGATGGTCATTATTGG
  TGAGCAAGAGCGGAATGAAGGTGTCATCAAGCTCCGTTCCGTGGCCAGCAGAGAAGAAGTGACCATTAAT
  CGAGAAAGTCTCGTGGCTGAAATTCAGAAGCGACTGTCTGAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208134 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPHLGPLRRRAWAALLGQLLRPPSTVCTRGCHSQVAKAVLTSEQLKSHQEKPNFVIKVPKGTRDLSPQQM
  VVREKILDKIISCFKRHGAKGLDTPAFELKEMLTEKYEDNFGLMYDLKDQGGELLSLRYDLTVPFARYLA
  MNKLKKMKRYQVGKVWRRESPAIAQGRYREFCQCDFDIAGEFDPMIPDAECLRIMCEILSGLQLGDFLIK
  VNDRRVVDGIFAVCGVPESKLRTICSSMDKLDKMSWEGVRHEMVAKKGLAPEVADRIGDFVQYHGGISLV
  EDLFKDPRLSQSQLALQGLGDLKLLFEYLRLFGIADKISLDLSLARGLDYYTGVIYEAVLLESPAQAGKE
  TLSVGSVAAGGRYDNLVAQFDPKGHHVPCVGLSIGVERIFYLVEQKMKMSGEKVRTTETQVFVATPQKNF
  LQERLKIIAELWDAGIKAEMLYKNNPKLLTQLHYCEKADIPLMVIIGEQERNEGVIKLRSVASREEVTIN
  RESLVAEIQKRLSES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080636
• ORF Size: 1518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080636.1, NM_080636.2, NP_542367.1
• RefSeq Size: 3075 bp
• RefSeq ORF: 1518 bp
• Locus ID: 70791
• MW: 57 kDa
• Gene Summary: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of histidine to tRNA molecules. Mutations in a similar gene in human have been associated with Perrault syndrome 2 (PRLTS2). [provided by RefSeq, Mar 2015]