Acvr1 (NM_007394) Mouse Tagged ORF Clone

CAT#: MR208188

Acvr1 (Myc-DDK-tagged) - Mouse activin A receptor, type 1 (Acvr1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acvr1
• Synonyms: ActR-I; ActRIA; Acvr; Acvr1a; Acvrlk2; Alk-2; ALK2; Alk8; D330013D15Rik; SKR1; Tsk7L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGATGGAGTAATGATCCTTCCTGTGCTAATGATGATGGCTTTCCCTTCCCCGAGTGTGGAAGATG
  AGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCA
  CTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGAGCATCAACGATGGCTTCCACGTCTACCAGAAGGGCTGC
  TTTCAGGTTTATGAGCAGGGGAAGATGACGTGTAAGACCCCGCCGTCACCTGGCCAGGCTGTGGAGTGCT
  GCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCTGCCCACTAAAGGGAAGTCCTTCCCCGGAAC
  ACAGAATTTCCACCTGGAAGTTGGCCTTATCATCCTCTCGGTGGTGTTTGCAGTATGTCTTTTAGCTTGC
  ATCCTTGGAGTTGCTCTCAGGAAGTTTAAGAGACGCAATCAAGAGCGCCTGAACCCCAGAGACGTGGAGT
  ATGGTACCATTGAAGGGCTCATCACCACCAATGTGGGAGACAGCACTCTAGCGGAACTACTAGATCACTC
  GTGTACATCAGGAAGTGGCTCCGGTCTTCCTTTCCTGGTACAGAGAACGGTGGCTCGCCAGATAACCCTG
  TTGGAGTGTGTCGGGAAGGGCCGGTATGGAGAAGTATGGAGGGGCAGCTGGCAAGGCGAAAATGTCGCTG
  TGAAGATCTTCTCCTCCCGAGACGAGAAGTCATGGTTCAGGGAGACGGAATTGTACAACACTGTGATGTT
  GAGGCATGAAAATATCTTAGGTTTCATCGCTTCAGACATGACCTCCAGACACTCCAGTACCCAGCTGTGG
  CTCATCACACATTACCATGAAATGGGATCGTTGTATGACTACCTTCAGCTCACTACTCTGGATACGGTTA
  GCTGCCTTCGGATTGTACTGTCCATAGCCAGCGGCCTTGCCCATTTGCACATAGAGATATTTGGGACCCA
  AGGGAAGTCCGCCATTGCCCATCGAGATCTGAAGAGCAAAAACATCCTGGTGAAGAAGAATGGACAGTGC
  TGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACAAACCAGCTTGATGTGGGAAACAACC
  CCCGTGTGGGGACCAAGCGCTACATGGCTCCGGAAGTGCTCGATGAAACCATCCAAGTGGATTGCTTTGA
  TTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGC
  AATGGTATAGTGGAAGATTACAAGCCACCATTCTATGATGTGGTTCCCAATGACCCAAGTTTTGAAGATA
  TGAGGAAAGTTGTCTGTGTGGATCAACAGAGGCCAAACATACCTAACAGATGGTTCTCAGACCCGACATT
  AACTTCTCTGGCGAAGCTGATGAAAGAGTGCTGGTATCAGAACCCATCCGCAAGACTCACAGCTCTACGT
  ATCAAAAAGACTTTGACCAAAATCGATAATTCCCTAGACAAATTAAAAACTGACTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDGVMILPVLMMMAFPSPSVEDEKPKVNQKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGC
  FQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLAC
  ILGVALRKFKRRNQERLNPRDVEYGTIEGLITTNVGDSTLAELLDHSCTSGSGSGLPFLVQRTVARQITL
  LECVGKGRYGEVWRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLW
  LITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKSAIAHRDLKSKNILVKKNGQC
  CIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVS
  NGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRPNIPNRWFSDPTLTSLAKLMKECWYQNPSARLTALR
  IKKTLTKIDNSLDKLKTDC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007394
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007394.1, NM_007394.2, NM_007394.3, NP_031420.2
• RefSeq Size: 3067 bp
• RefSeq ORF: 1530 bp
• Locus ID: 11477
• MW: 57.2 kDa