Lyn (NM_001111096) Mouse Tagged ORF Clone

CAT#: MR208225

Lyn (Myc-DDK-tagged) - Mouse Yamaguchi sarcoma viral (v-yes-1) oncogene homolog (Lyn), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lyn
• Synonyms: AA407514; Hck-2; p53Lyn; p56Lyn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208225 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATGTATTAAATCAAAAAGGAAAGACAATCTCAATGACGATGAAGTAGATTCGAAGACTCAACCAG
  TACGTAATACTGACCGAACTATTTATGTGAGAGATCCAACGTCCAATAAACAGCAAAGGCCAGTTCCTGA
  ATTTCATCTTTTACCAGGACAGAGATTTCAAACAAAAGATCCAGAGGAACAAGGTGACATTGTGGTGGCC
  TTATACCCTTATGATGGCATCCACCCAGATGACTTGTCCTTCAAGAAAGGAGAAAAGATGAAAGTTCTGG
  AAGAGCATGGGGAATGGTGGAAAGCTAAGTCCCTTTCATCAAAGAGAGAAGGCTTCATCCCCAGCAACTA
  CGTGGCCAAGGTCAACACCTTAGAAACTGAAGAGTGGTTCTTCAAGGACATAACAAGGAAAGATGCAGAG
  CGACAGCTTCTGGCACCAGGAAACAGTGCAGGAGCTTTCCTTATCAGAGAAAGCGAAACTTTAAAGGGAA
  GCTTCTCTCTTTCTGTCAGAGATTATGACCCTATGCATGGTGATGTCATTAAGCACTACAAAATTAGAAG
  TCTGGACAATGGTGGCTATTACATCTCTCCTCGCATCACTTTTCCCTGCATCAGTGACATGATTAAGCAT
  TACCAAAAGCAGTCTGATGGTCTATGCAGAAGACTGGAGAAGGCATGCATCAGTCCCAAACCTCAGAAGC
  CATGGGATAAAGATGCCTGGGAGATCCCCCGGGAGTCCATTAAGTTGGTGAAAAAGCTTGGCGCAGGGCA
  GTTTGGGGAAGTCTGGATGGGTTACTATAACAACAGCACAAAGGTGGCTGTGAAGACCCTCAAGCCCGGC
  ACCATGTCTGTGCAGGCATTCCTGGAAGAGGCCAACCTCATGAAGACCTTGCAGCATGACAAGCTAGTGC
  GGCTGTACGCTGTGGTCACCAAGGAGGAGCCCATCTACATCATCACCGAGTTCATGGCTAAGGGTAGTTT
  GCTGGATTTCCTCAAGAGTGATGAAGGTGGCAAGGTGCTGCTGCCCAAGCTCATTGACTTCTCGGCCCAG
  ATTGCAGAAGGCATGGCGTACATCGAGCGGAAGAACTACATCCACCGTGATCTGCGAGCTGCAAACGTCC
  TGGTCTCTGAGTCACTCATGTGCAAGATTGCAGACTTTGGCCTCGCGAGAGTCATCGAAGATAACGAGTA
  CACAGCAAGGGAAGGTGCGAAGTTCCCTATCAAGTGGACAGCTCCAGAGGCCATCAACTTCGGCTGCTTC
  ACTATCAAATCTGACGTGTGGTCCTTCGGAATTCTCCTGTATGAGATTGTCACCTATGGGAAGATTCCCT
  ACCCAGGGAGAACCAACGCAGATGTGATGAGCGCACTGTCACAGGGATATCGAATGCCACGCATGGAGAA
  CTGCCCAGATGAGCTCTATGACATCATGAAAATGTGTTGGAAAGAAAAGGCAGAGGAGAGGCCAACTTTT
  GACTACTTACAGAGTGTCCTGGATGACTTCTATACAGCCACAGAAGGGCAGTATCAGCAGCAACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208225 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGCIKSKRKDNLNDDEVDSKTQPVRNTDRTIYVRDPTSNKQQRPVPEFHLLPGQRFQTKDPEEQGDIVVA
  LYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLSSKREGFIPSNYVAKVNTLETEEWFFKDITRKDAE
  RQLLAPGNSAGAFLIRESETLKGSFSLSVRDYDPMHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKH
  YQKQSDGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKKLGAGQFGEVWMGYYNNSTKVAVKTLKPG
  TMSVQAFLEEANLMKTLQHDKLVRLYAVVTKEEPIYIITEFMAKGSLLDFLKSDEGGKVLLPKLIDFSAQ
  IAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCF
  TIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMSALSQGYRMPRMENCPDELYDIMKMCWKEKAEERPTF
  DYLQSVLDDFYTATEGQYQQQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111096
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111096.1, NP_001104566.1
• RefSeq Size: 3456 bp
• RefSeq ORF: 1539 bp
• Locus ID: 17096
• MW: 58.8 kDa