Gba (NM_008094) Mouse Tagged ORF Clone

CAT#: MR208259

Gba (Myc-DDK-tagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gba
• Synonyms: betaGC; GBA1; GC; GCase; GLUC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208259 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGCA
  TCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGACCC
  CGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAGCTG
  AGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAGAAGT
  TCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTGTCCCC
  ACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATCCGGGTA
  CCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTCCAGTTAT
  CCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTGAAGATGTC
  CTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAAGAGTG
  AATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAATTACTTTGTCA
  AGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAATGAACCTACGGC
  AGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGAGACTTCATTTCC
  CGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTAGATGACCAACGCT
  TGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTACGTTCATGGCATTGC
  TGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
  AACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAGAGTGTTCGGTTGGGCT
  CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
  GGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTTGTCGATAGCCCCATT
  ATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
  TCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACTGACTTGGAAACAGTAGCACT
  GTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCGGAGGATGTCCCTCTTACCATC
  AGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCCATTCACACTTACCTGTGGCGTC
  GCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208259 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAARLIGFFLFQAVSWAYGAQPCIPKSFGYSSVVCVCNASYCDSLDPVTLPALGTFSRYESTRRGRRMEL
  SVGAIQANRTGTGLLLTLQPEKKFQKVKGFGGAMTDATALNILALSPPTQKLLLRSYFSTNGIEYNIIRV
  PMASCDFSIRVYTYADTPNDFQLSNFSLPEEDTKLKIPLIHQALKMSSRPISLFASPWTSPTWLKTNGRV
  NGKGSLKGQPGDIFHQTWANYFVKFLDAYAKYGLRFWAVTAENEPTAGLFTGYPFQCLGFTPEHQRDFIS
  RDLGPALANSSHDVKLLMLDDQRLLLPRWAEVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFP
  NTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPI
  IVDIPKDAFYKQPMFYHLGHFSKFIPEGSQRVALVASESTDLETVALLRPDGSAVVVVLNRSSEDVPLTI
  SDPDLGFLETVSPGYSIHTYLWRRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008094
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008094.1, NM_008094.2, NM_008094.3, NM_008094.4, NM_008094.5, NP_032120.1
• RefSeq Size: 2238 bp
• RefSeq ORF: 1548 bp
• Locus ID: 14466
• MW: 57.6 kDa