Apex2 (NM_029943) Mouse Tagged ORF Clone

CAT#: MR208278

Apex2 (Myc-DDK-tagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apex2
• Synonyms: ape2; C430040P13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208278 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCGTGGTAAGCTGGAACATCAATGGGATCCGGAGTCCCCTGCAAGGCCTTGCATGCCAGGAAC
  CCAGCAGCTGTCCCACGGCCTTGCGACGCGTTTTGGACGAGCTGGATGCTGATATTGTCTGTCTCCAGGA
  GACCAAAGTGACCAGAGATGTACTGACAGAGCCCCTGGCTATTGTTGAGGGTTATAACTCCTATTTCAGC
  TTCAGCCGCAGCCGTAGTGGCTATTCTGGTGTGGCTACCTTCTGTAAGGACAGTGCTACCCCAGTAGCTG
  CTGAAGAAGGCCTGAGTGGTGTGTTTGCCACCCTGAATGGAGATATTGGTTGCTATGGAAACATGGATGA
  GTTCACGCAAGAGGAACTCCGGGTTCTGGATAGTGAGGGCCGGGCCCTCCTTACACAGCACAAGATCCGC
  ACATTGGAAGGGAAGGAGAAGACTTTGACCCTGATCAATGTGTACTGCCCTCACGCGGATCCTGGGAAGC
  CTGAGCGGCTGACCTTCAAGATGCGCTTCTATCGCCTGCTCCAGATGCGAGCAGAAGCGCTCCTGGCAGC
  TGGCAGTCACGTGATAATCCTGGGGGACCTGAATACAGCCCACCGACCCATTGACCACTGCGATGCAAGT
  AGTCTGGAATGCTTTGAAGAGGACCCAGGGCGTAAGTGGATGGATGGCTTGCTCAGTAACCCAGGGGATG
  AGGCTGGACCCCACATAGGGCTCTTCATGGATAGCTACCGCTACTTGCATCCAAAACAGCAGAGGGCTTT
  CACCTGCTGGTCAGTGGTCAGTGGTGCACGCCATCTCAACTATGGCTCTAGACTTGACTATGTACTGGGA
  GATAGGGCCTTGGTCATAGATACCTTCCAGGCCTCCTTCTTACTCCCTGAAGTGATGGGCTCTGACCACT
  GCCCTGTGGGAGCTGTCTTAAATGTATCCTGTGTGCCAGCAAAACAGTGCCCAGCTCTGTGTACCCGCTT
  CCTCCCCGAGTTTGCAGGTACCCAGCTCAAGATTCTTCGCTTCCTAGTTCCTCTTGAGCAAGAACCTGTG
  CGGGAACAGCAAGTCCTGCAGCCCAGCCATCAAATCCAGGCACAGAGACAGCCAAGAAAAGCCTGCATGC
  ATTCAACCAGGCTTCGGAAAAGTCAAGGTGGCCCCAAAAGAAAACAGAAAAACCTGATGAGTTACTTCCA
  GCCTTCCTCCAGCCTTTCCCAAACTTCTGGTGTGGAGCTGCCTACCCTGCCTCTGGTGGGCCCTCTTACA
  ACCCCAAAGACTGCAGAAGAGGTGGCAACAGCCACAGTGCTAGAAGAGAAGAACAAGGTTCCAGAGTCCA
  AAGATGAGAAGGGAGAAAGGACTGCCTTCTGGAAGTCTATGCTGAGTGGGCCCTCACCCATGCCCCTCTG
  TGGAGGCCACAGGGAGCCATGTGTGATGCGTACTGTGAAAAAAACAGGACCCAACTTTGGTCGCCAGTTC
  TACATGTGTGCTAGACCCCGAGGTCCTCCCAGTGACCCCTCTTCCCGCTGTAACTTCTTCCTCTGGAGCA
  GGCCCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208278 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRVVSWNINGIRSPLQGLACQEPSSCPTALRRVLDELDADIVCLQETKVTRDVLTEPLAIVEGYNSYFS
  FSRSRSGYSGVATFCKDSATPVAAEEGLSGVFATLNGDIGCYGNMDEFTQEELRVLDSEGRALLTQHKIR
  TLEGKEKTLTLINVYCPHADPGKPERLTFKMRFYRLLQMRAEALLAAGSHVIILGDLNTAHRPIDHCDAS
  SLECFEEDPGRKWMDGLLSNPGDEAGPHIGLFMDSYRYLHPKQQRAFTCWSVVSGARHLNYGSRLDYVLG
  DRALVIDTFQASFLLPEVMGSDHCPVGAVLNVSCVPAKQCPALCTRFLPEFAGTQLKILRFLVPLEQEPV
  REQQVLQPSHQIQAQRQPRKACMHSTRLRKSQGGPKRKQKNLMSYFQPSSSLSQTSGVELPTLPLVGPLT
  TPKTAEEVATATVLEEKNKVPESKDEKGERTAFWKSMLSGPSPMPLCGGHREPCVMRTVKKTGPNFGRQF
  YMCARPRGPPSDPSSRCNFFLWSRPS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029943
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029943.1, NM_029943.2, NP_084219.1
• RefSeq Size: 1903 bp
• RefSeq ORF: 1551 bp
• Locus ID: 77622
• MW: 57.3 kDa