Pfkfb2 (NM_008825) Mouse Tagged ORF Clone

CAT#: MR208305

Pfkfb2 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkfb2
• Synonyms: 4930568D07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208305 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAATAGTACATTTTCCCCAGAAGACTGCAACAGCAGCTATAAACCCCACGCCTCAAATCTGC
  GAAGGGCAGGGAAGACATGCTCATGGGCTTCCTATATGACCAACTCCCCAACACTCATTGTTATGATTGG
  CTTGCCAGCCCGGGGTAAAACTTATGTGTCTAAGAAACTAACACGCTACCTCAACTGGATTGGAGTACCC
  ACCAAAGTGTTTAATCTTGGGGTATATCGACGTGAAGCAGTCAAGTCCTATCAGTCTTATGATTTCTTTC
  GACATGACAATGAAGAGGCTATGAAGATCCGCAAACAGTGTGCTCTGGTGGCACTGGAAGATGTTAAAGC
  CTATTTTACTGAAGAGAGTGGGCAGATTGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGGGAC
  ATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTTTTTGTAGAATCTGTCTGTGATGATCCTG
  ATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTTTCGAGCCCTGACTACCCCGAAAGGAATAGGGAGAA
  TGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGACAAC
  TATGATAAGGATCTTTCTTTCATAAAGGTGATAAATGTAGGCCAGAGATTCCTGGTCAACAGAGTTCAGG
  ACTACATCCAGAGTAAGATTGTCTACTACCTGATGAATATCCACGTCCATCCTCGCACCATCTACCTTTG
  CCGGCACGGCGAGAGCGAGTTCAACCTTTTGGGGAAGATTGGGGGTGACTCTGGCCTCTCCGTGCGAGGA
  AAGCAGTTTGCTCATGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTTAAAGTGTGGACGA
  GCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAGTGGAAGATCCTCAA
  TGAGATTGATGCTGGCGTGTGTGAGGAGATGACATATTCAGAGATTGAGCAACGGTACCCAGAGGAATTT
  GCACTCCGAGATCAAGAGAAGTATCTGTACCGATACCCTGGTGGGGAGTCCTACCAGGACCTGGTGCAGC
  GGCTGGAGCCTGTCATCATGGAGCTAGAGCGTCAAGGCAACATCCTCGTTATCTCTCACCAGGCTGTCAT
  GCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTACCATACTTGAGGTGCCCCCTGCAC
  ATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACTCTGAATGTGGATGCTG
  TAGACACACATCGTGACAAGCCAACTCACAACTTCCCCAAGAGCCAAACCCCTGTAAGGATGAGAAGGAA
  CAGCTTTACGCCTCTGTCCAGTTCGAACACAATAAGGCGTCCAAGAAATTACAGTGTTGGGAGCCGACCC
  CTCAAGCCCCTCAGCCCTCTCCGTGCTCTGGACATGCAAGAAGGGGCGGACCAGCCGAAGACCCAAGTCA
  GCATTCCGGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208305 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIVMIGLPARGKTYVSKKLTRYLNWIGVP
  TKVFNLGVYRREAVKSYQSYDFFRHDNEEAMKIRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRD
  MILNFAKQNAFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDN
  YDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGLSVRG
  KQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVCEEMTYSEIEQRYPEEF
  ALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVISHQAVMRCLLAYFLDKGADELPYLRCPLH
  IIFKLTPVAYGCKVETITLNVDAVDTHRDKPTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRP
  LKPLSPLRALDMQEGADQPKTQVSIPVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008825
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008825.2, NM_008825.3, NM_008825.4, NP_032851.2
• RefSeq Size: 7308
• RefSeq ORF: 1557
• Locus ID: 18640
• MW: 59.8 kDa
• Gene Summary: Synthesis and degradation of fructose 2,6-bisphosphate. [UniProtKB/Swiss-Prot Function]