Aldh1b1 (NM_028270) Mouse Tagged ORF Clone

CAT#: MR208320

Aldh1b1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 1 family, member B1 (Aldh1b1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh1b1
• Synonyms: 2700007F14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208320 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACTGCCCGACTCTTGCTGCCCCGGCTCCTCTGCCTCCAGGGCAGGACTACCTCTTACTCTACAG
  CAGCTGCTCTCCCGAACCCAATCCCAAACCCAGAGATTTGCTACAACAAGCTGTTCATCAACAACGAGTG
  GCATGATGCGGTCAGCAAAAAGACCTTCCCCACAGTGAACCCCACTACAGGTGAGGTCATTGGGCATGTG
  GCCGAAGGTGACCGGGCAGATGTGGATCTGGCTGTAAAAGCAGCCCGAGAAGCCTTCCGCCTGGGGTCCC
  CATGGCGCAGGATGGATGCCTCAGAGCGGGGCCGGCTGCTGAACCGCCTAGCTGATCTTGTGGAACGAGA
  TCGAGTGTACTTGGCCTCACTGGAGACGCTAGATAACGGGAAACCTTTCCAGGAGTCTTATGTCTTGGAT
  CTGGATGAAGTCATCAAGGTGTACCGTTACTTCGCTGGCTGGGCTGACAAGTGGCATGGTAAGACCATCC
  CTATGGATGGTGAGCATTTCTGCTTCACCCGACATGAGCCAGTGGGTGTCTGTGGCCAGATAATCCCTTG
  GAACTTCCCACTGGTCATGCAGGGCTGGAAGCTGGCCCCGGCACTCGCCACGGGCAACACTGTGGTCATG
  AAGGTGGCAGAGCAGACCCCACTCTCTGCTCTGTACTTGGCCTCCCTCATCAAAGAGGCGGGGTTTCCCC
  CAGGAGTGGTGAACATCATCACTGGCTACGGCCCCACGGCGGGAGCTGCCATCGCTCAGCACATGGATGT
  GGATAAAGTCGCCTTCACGGGCTCCACTGAGGTAGGCCACCTGATTCAGAAGGCAGCTGGCGAGTCTAAC
  CTCAAGAGAGTCACCCTGGAGCTGGGTGGGAAGAGCCCCAGCATTGTGCTGGCAGACGCTGACATGGAGC
  ATGCCGTAGATCAGTGTCACGAAGCCCTTTTCTTCAACATGGGCCAGTGCTGCTGTGCAGGCTCCCGGAC
  ATTCGTGGAAGAGTCCATCTACCGTGAGTTTCTCGAGAGAACTGTGGAGAAGGCCAAGCAGAGGAAAGTG
  GGGAACCCCTTTGAGTTGGACACCCAGCAGGGACCTCAGGTGGACAAGGAGCAGTTTGAACGAATCCTGG
  GCTACATCCGGCTGGGACAGAAGGAAGGGGCAAAGCTTCTCTGTGGCGGGGAGCGTTTGGGGGAGCGCGG
  CTTCTTCATCAAACCCACAGTCTTCGGGGACGTTCAGGATGGCATGAGGATCGCCAAGGAGGAGATCTTT
  GGGCCCGTGCAGCCTCTGTTCAAGTTCAAGAAGATCGAGGAAGTAATCCAGAGAGCCAACAACACCAGGT
  ATGGCCTGGCTGCGGCTGTGTTCACCCGAGACCTGGACAAGGCCATCTACTTCACGCAGGCCCTGCAAGC
  TGGGACGGTGTGGGTGAACACCTATAACATTGTCACCTGCCACACGCCATTCGGAGGCTTTAAGGAATCT
  GGCAATGGCAGGGAGCTGGGGGAGGACGGGCTCAGAGCCTACACGGAGGTGAAGACTGTCACCATCAAGG
  TTCCCGAGAAGAATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208320 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLTARLLLPRLLCLQGRTTSYSTAAALPNPIPNPEICYNKLFINNEWHDAVSKKTFPTVNPTTGEVIGHV
  AEGDRADVDLAVKAAREAFRLGSPWRRMDASERGRLLNRLADLVERDRVYLASLETLDNGKPFQESYVLD
  LDEVIKVYRYFAGWADKWHGKTIPMDGEHFCFTRHEPVGVCGQIIPWNFPLVMQGWKLAPALATGNTVVM
  KVAEQTPLSALYLASLIKEAGFPPGVVNIITGYGPTAGAAIAQHMDVDKVAFTGSTEVGHLIQKAAGESN
  LKRVTLELGGKSPSIVLADADMEHAVDQCHEALFFNMGQCCCAGSRTFVEESIYREFLERTVEKAKQRKV
  GNPFELDTQQGPQVDKEQFERILGYIRLGQKEGAKLLCGGERLGERGFFIKPTVFGDVQDGMRIAKEEIF
  GPVQPLFKFKKIEEVIQRANNTRYGLAAAVFTRDLDKAIYFTQALQAGTVWVNTYNIVTCHTPFGGFKES
  GNGRELGEDGLRAYTEVKTVTIKVPEKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028270
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028270.1, NM_028270.2, NM_028270.3, NM_028270.4, NP_082546.1
• RefSeq Size: 2302 bp
• RefSeq ORF: 1560 bp
• Locus ID: 72535
• MW: 57.6 kDa