Ptbp3 (NM_144904) Mouse Tagged ORF Clone

CAT#: MR208328

Ptbp3 (Myc-DDK-tagged) - Mouse ROD1 regulator of differentiation 1 (S. pombe) (Rod1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptbp3
• Synonyms: 5830471K22Rik; AA407443; AI462022; AW107884; C86549; Rod1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208328 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCTCGACTTCTGCAGCTAATGGGAATGACAACAAGAAATTTAAAGGAGATAGACCTCCCTGTT
  CGCCTTCCCGTGTTCTCCATCTTCGAAAAATTCCATGTGATGTCACCGAAGCAGAGGTCATATCATTAGG
  TCTACCATTTGGCAAAGTAACTAATCTTTTGATGTTGAAAGGAAAAAGCCAGGCCTTTTTAGAAATGGCT
  TCTGAAGAAGCTGCTGTTACTATGATAAATTATTACACTCCTGTTACTCCTCATCTTCGAAGTCAGCCTG
  TTTATATCCAGTATTCCAATCACCGAGAACTTAAGACTGACAATCTGCCTAATCAGGCTAGAGCCCAAGC
  TGCACTGCAGGCTGTCAGTGCAGTCCAGTCAGGAAACTTGTCCCTTCCTGGAGCTACTGCAAATGAAGGC
  ACATTGCTACCTGGGCAGAGCCCTGTGCTCCGGATAATTATTGAAAACCTATTTTACCCTGTCACTCTGG
  AAGTTCTCCATCAGATATTTTCTAAATTTGGCACGGTGTTGAAGATTATCACCTTTACAAAGAATAATCA
  GTTTCAAGCCTTGCTTCAGTATGCCGACCCAGTGAATGCGCAGTATGCCAAAATGGCTCTGGATGGCCAG
  AATATCTACAATGCGTGCTGCACTCTCCGTATTGACTTCTCCAAGCTCACCAGCCTTAATGTGAAATACA
  ATAATGACAAAAGCAGAGACTTCACTCGCTTAGACCTTCCTACTGGTGACGGCCAGCCATCTCTTGAACC
  TCCTATGGCTGCTGCTTTTGGTGCACCGGGTATAATGTCCTCTCCATATGCAGGGGCTGCTGGCTTTGCC
  CCAGCCATCGCATTTCCTCAAGCAGCAGGTCTGTCTGTCCCAGCTGTTCCCGGAGCCCTTGGTCCTCTCA
  CACTTACCTCCTCTGCTGTCAGTGGAAGGATGGCCATTCCTGGGGCAAGTGGTATGCCTGGGAATTCTGT
  TCTGCTGGTCACCAACCTCAATCCTGACTTTATCACACCACATGGGCTTTTTATCCTATTTGGAGTGTAT
  GGTGATGTACATCGAGTGAAGATTATGTTTAACAAGAAAGAAAACGCCTTGGTGCAGATGGCGGATGCGA
  GTCAAGCCCAGCTAGCAATGAACCACCTAAGTGGTCAGAGGCTCTATGGAAAAGTACTTCGGGCTACACT
  GTCCAAACATCAAGCCGTCCAGCTCCCTAGAGAGGGACAGGAAGACCAAGGTCTGACCAAGGACTTCAGC
  AATAGTCCCCTGCATCGCTTTAAAAAGCCCGGCTCAAAGAATTTCCAAAATATCTTCCCTCCATCAGCCA
  CACTTCATCTTTCCAACATTCCTCCTTCTGTCACAATGGATGACCTGAAGAACCTGTTCACAGAAGCTGG
  ATGCTCAGTGAAGGCTTTTAAGTTCTTCCAGAAAGACCGTAAGATGGCTCTCATTCAGTTGGGATCTGTG
  GAAGAAGCAATCCAGGCTCTTATTGAGCTTCACAATCATGACCTTGGAGAGAATCACCACCTCAGAGTTT
  CCTTCTCAAAATCCACCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208328 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSSTSAANGNDNKKFKGDRPPCSPSRVLHLRKIPCDVTEAEVISLGLPFGKVTNLLMLKGKSQAFLEMA
  SEEAAVTMINYYTPVTPHLRSQPVYIQYSNHRELKTDNLPNQARAQAALQAVSAVQSGNLSLPGATANEG
  TLLPGQSPVLRIIIENLFYPVTLEVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVNAQYAKMALDGQ
  NIYNACCTLRIDFSKLTSLNVKYNNDKSRDFTRLDLPTGDGQPSLEPPMAAAFGAPGIMSSPYAGAAGFA
  PAIAFPQAAGLSVPAVPGALGPLTLTSSAVSGRMAIPGASGMPGNSVLLVTNLNPDFITPHGLFILFGVY
  GDVHRVKIMFNKKENALVQMADASQAQLAMNHLSGQRLYGKVLRATLSKHQAVQLPREGQEDQGLTKDFS
  NSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVTMDDLKNLFTEAGCSVKAFKFFQKDRKMALIQLGSV
  EEAIQALIELHNHDLGENHHLRVSFSKSTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144904
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144904.1
• RefSeq Size: 6880 bp
• RefSeq ORF: 1656 bp
• Locus ID: 230257
• MW: 56.4 kDa