Arfgap2 (NM_023854) Mouse Tagged ORF Clone

CAT#: MR208335

Arfgap2 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arfgap2
• Synonyms: 2310032E02Rik; Zfp289
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208335 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGAGCCCGAGCAAGACCGAAATCCAGACTATTTTTAAGAGACTTCGCGCAATTCCCACCAACA
  AGGCCTGTTTCGATTGTGGCGCCAAGAGTCCTAGTTGGGCCAGCATCACTTACGGTGTATTTTTGTGTAT
  TGACTGCTCCGGAGTGCATCGCTCCTTGGGTGTCCATCTCAGCTTTATCAGGTCCACAGAGTTGGATTCG
  AACTGGAGCTGGTTGCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACTGCTTTTTTCCGCC
  AACACGGATGTATGGCCAATGATGCTAACACCAAATATACGAGCCGAGCTGCCCAGATGTACCGAGAGAA
  GATCCGGCAGTTAGGGAGTGCAGCTCTGACTAGGCATGGTACTGATCTTTGGATAGACAGTATGAACAGT
  GCTCCAAGTCACTCTCCAGAGAAGAAAGACTCTGATTTCTTCACAGAACATACTCAGGCCCCTGCTTGGG
  ATACAGCAGCCACTGATCCTTCAGGGACTCAGCAACCAGCCCTACCTTCAGAGAGCAGTAGCCTGGCACA
  ACCTGAACAAGGCCCTAACACTGATCTGCTCGGAACCTCACCCCAAGCCTCTCTGGAACTGAAAAGCTCC
  ATTATTGGCAAGAAGAAGCCAGCAGCCGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTGGGAGCTCAGA
  AGGTGAGCAACCAGAGCTTTACTGAGATTGAGCGGCAGGCCCAGGTGGCAGAGAAGCTCCGAGAGCAGCA
  GGCAGCTGATGCCAAGAAGCAGGCAGAAGAGTCCATGGTTGCCTCCATGCGTCTGGCCTACCAGGAGCTC
  CAGATTGATCGCAAGAAGGAGGAGAAAAAGCTCCAGAATCTTGAAGGAAAGAAGCGAGAGCAGGCTGAAA
  GGCTGGGCATGGGCTTGGTGTCCCGAAGCTCCATCTCCCATTCTGTGCTGTCTGAGATGCAGATGATTGA
  GCAGGAAACCCCGCTGAGTGCGAAATCTTCCCGTTCACAGCTTGACTTGTTTGATGATGTTGGAACATTT
  GCATCTGGACCCCCAAAGTACAAGGACAACCCCTTTTCCTTGGGAGAAACTTTTGGTTCCCGCTGGGATT
  CAGATGCAGCCTGGGGTATGGACAGGGTAGAAGAGAAGGAACCTGAAGTTACCATCTCCAGCATCCGGCC
  TATTTCAGAAAGAACTGCGAGTCGGAGGGAAGTGGAGACTCGGAGCTCAGGCCTTGAGTCCAGTGAAGCA
  CGGCAGAAGTTTGCAGGAGCCAAAGCCATCTCGTCCGACATGTTCTTTGGTCGTGAGGTGGATTCTGAGT
  ATGAAGCTAGGTCTCGGTTACAGCAGCTTTCGGGCAGCAGTGCCATCAGCTCTTCAGACCTTTTTGGAAA
  CATGGATGGAGCTCATGGAGGAGGAACTGTATCTCTGGGGAATGTACTTCCTACAGCTGACATTGCCCAG
  TTTAAGCAGGGTGTCAAGTCTGTGGCTGGCAAGATGGCTGTGCTGGCCAATGGTGTGATGAATTCCTTAC
  AGGATCGCTACGGTTCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208335 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASPSKTEIQTIFKRLRAIPTNKACFDCGAKSPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDS
  NWSWLQLRCMQVGGNANATAFFRQHGCMANDANTKYTSRAAQMYREKIRQLGSAALTRHGTDLWIDSMNS
  APSHSPEKKDSDFFTEHTQAPAWDTAATDPSGTQQPALPSESSSLAQPEQGPNTDLLGTSPQASLELKSS
  IIGKKKPAAAKKGLGAKKGLGAQKVSNQSFTEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQEL
  QIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSISHSVLSEMQMIEQETPLSAKSSRSQLDLFDDVGTF
  ASGPPKYKDNPFSLGETFGSRWDSDAAWGMDRVEEKEPEVTISSIRPISERTASRREVETRSSGLESSEA
  RQKFAGAKAISSDMFFGREVDSEYEARSRLQQLSGSSAISSSDLFGNMDGAHGGGTVSLGNVLPTADIAQ
  FKQGVKSVAGKMAVLANGVMNSLQDRYGSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023854
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023854.1, NM_023854.2, NP_076343.1, NP_076343.2
• RefSeq Size: 3367 bp
• RefSeq ORF: 1563 bp
• Locus ID: 77038
• MW: 56.6 kDa
• Gene Summary: This gene encodes a zinc-finger-containing GTPase-activating protein for ADP ribosylation factor 1 (ARF1), a small GTPase that plays a role in coatomer-mediated vesicular trafficking. This gene product stimulates the hydrolysis of ARF1-bound GTP, which may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. It may regulate the retrograde transport from the Golgi complex to the endoplasmic reticulum. Expression of this gene has been shown to be controlled by inhibitor of DNA binding 1 (Id1). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 6. [provided by RefSeq, Oct 2009]