Prpf4 (NM_027297) Mouse Tagged ORF Clone

CAT#: MR208353

Prpf4 (Myc-DDK-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog (yeast) (Prpf4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prpf4
• Synonyms: 1600015H11Rik; AI874830; AW047464; bN189G18.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208353 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTCACGAGCCTCCTCCACGACAACCAAAACCAAAGCGCCTGACGATTTAGTTGCTCCCGTTG
  TGAAGAAACCACACATCTACTATGGAAGCTTGGAAGAGAAAGAGCGGGAACGCCTGGCCAAAGGAGAGTC
  TGGGATTTTGGGAAAAGAAGGACTCAAAGCCGGCATTGAAGCAGGAAATATTAATATAACATCCGGAGAA
  GTGTTTGAAATTGAGGAGCACATCAGTGAGCGGCAGGCAGAAGTCTTGGCAGAGTTTGAAAGAAGAAAGC
  GAGCCCGGCAGATCAATGTTTCCACAGATGACTCAGAGGTCAAGGCTTGCCTTCGAGCCTTGGGAGAGCC
  TATCACGCTTTTTGGAGAGGGCCCTGCTGAACGAAGAGAAAGGTTAAGAAATATTCTCTCAGTGGTCGGG
  ACTGATGCCTTAAAAAAGACGAAAAAAGATGATGAAAAGTCTAAGAAGTCAAAGGAGGAGTATCAGCAAA
  CCTGGTACCATGAAGGGCCAAATAGCCTGAAGGTGGCCAGATTGTGGATTGCTAATTATTCACTCCCCAG
  GGCAATGAAACGCTTAGAGGAGGCCCGTCTCCATAAGGAGATTCCTGAGACAACCAGAACCTCCCAGATG
  CAGGAGCTGCACAAGTCTCTCCGGTCTTTGAATAATTTCTGCAGTCAGATTGGAGATGACCGGCCGATCT
  CCTACTGCCACTTCAGCCCCAATTCTAAAATGCTAGCCACAGCTTGTTGGAGTGGGCTTTGCAAGCTCTG
  GTCTGTCCCTGACTGCAGCCTCCTTCACACTCTTCGAGGCCATAACACAAATGTAGGAGCAATTGTATTC
  CATCCCAAATCCACTGTCTCGTTGGACCAGAAGGATGTCAACCTGGCCTCTTGTGCTGCTGATGGTTCTG
  TGAAGCTTTGGAGCCTTGACAGCGATGAACCAGTGGCAGATATTGAAGGCCATACAGTGCGTGTAGCTCG
  GGTAATGTGGCATCCATCAGGGCGTTTCCTGGGCACAACCTGTTATGACCGTTCATGGCGCTTATGGGAT
  TTAGAGGCTCAAGAGGAGATCCTGCACCAGGAAGGCCACAGCATGGGTGTGTATGACATTGCCTTCCATC
  AAGATGGCTCTTTGGCTGGCACTGGGGGACTGGATGCATTTGGCCGGGTTTGGGACCTACGCACAGGACG
  CTGCATTATGTTCCTGGAAGGCCACCTGAAGGAAATCTATGGGATAAATTTCAGCCCCAATGGCTACCAC
  ATTGCCACTGGCAGTGGTGACAACACCTGCAAAGTGTGGGACCTTCGGCAGCGACGCTGTGTCTACACCA
  TCCCCGCCCATCAGAACTTAGTGACCGGTGTCAAATTTGAGCCTATCCATGGGGACTTCTTGCTCACAGG
  TGCCTATGACAATACAGCCAAGATCTGGACCCACCCAGGCTGGTCCCCACTGAAGACCCTGGCTGGTCAT
  GAGGGCAAAGTGATGGGCCTGGACATTTCTTCTGATGGGCAGCTCATAGCCACGTGCTCCTATGACAGAA
  CCTTCAAGCTCTGGATGGCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208353 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSRASSTTTKTKAPDDLVAPVVKKPHIYYGSLEEKERERLAKGESGILGKEGLKAGIEAGNINITSGE
  VFEIEEHISERQAEVLAEFERRKRARQINVSTDDSEVKACLRALGEPITLFGEGPAERRERLRNILSVVG
  TDALKKTKKDDEKSKKSKEEYQQTWYHEGPNSLKVARLWIANYSLPRAMKRLEEARLHKEIPETTRTSQM
  QELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCSLLHTLRGHNTNVGAIVF
  HPKSTVSLDQKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWD
  LEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYH
  IATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGDFLLTGAYDNTAKIWTHPGWSPLKTLAGH
  EGKVMGLDISSDGQLIATCSYDRTFKLWMAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027297
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027297.1, NM_027297.2, NM_027297.3, NP_081573.1
• RefSeq Size: 5237 bp
• RefSeq ORF: 1566 bp
• Locus ID: 70052
• MW: 58.4 kDa