Tmem63b (BC057136) Mouse Tagged ORF Clone

CAT#: MR208385

Tmem63b (Myc-DDK-tagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem63b
• Synonyms: BC026370
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208385 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCAACCCCAAACCGTGTGGTCACCTCTGCTGCTGTGTGGTTCGAGGCTGCGAGCAGGTGGAGGCCA
  TTGAGTACTACACAAAGCTGGAGCAGAGGCTGAAGGAAGACTACAGGCGGGAGAAGGAGAAAGTGAACGA
  GAAACCTCTGGGCATGGCCTTTGTCACCTTCCACAATGAGACCATCACAGCCATCATCCTAAAGGACTTC
  AACGTGTGCAAATGCCAGGGCTGTACCTGCCGCGGGGAGCCGAGAGCCTCCTCCTGCAGCGAAGCACTGC
  ATATCTCCAACTGGACCGTGACCTACGCCCCTGACCCTCAGAACATCTATTGGGAACACCTCTCCATCCG
  AGGCTTCATCTGGTGGCTGCGCTGCCTGGTCATCAATGTGGTCCTCTTCATTCTGCTCTTCTTCCTCACC
  ACCCCGGCCATCATCATCACCACCATGGACAAGTTCAACGTCACCAAGCCTGTGGAGTACCTCAACAACC
  CGATCATCACCCAGTTCTTCCCTACGCTACTGCTGTGGTGCTTCTCGGCCCTGCTCCCCACCATTGTCTA
  CTACTCCGCCTTCTTCGAAGCACACTGGACGCGCTCCGGGGAGAACAGGACGACCATGCACAAGTGCTAC
  ACCTTTCTCATCTTCATGGTGCTGCTTCTGCCCTCGCTGGGGCTGAGCAGCCTAGACCTCTTCTTCCGCT
  GGCTCTTTGACAAGAAATTCCTGGCTGAAGCAGCTATTCGGTTTGAGTGTGTGTTCCTGCCCGACAACGG
  CGCTTTCTTCGTGAACTACGTCATTGCCTCAGCCTTTATCGGTAACGCCATGGACCTGCTGCGCATCCCA
  GGCCTACTCATGTACATGATCCGGCTCTGCCTGGCGCGCTCCGCCGCTGAAAGACGCAACGTGAAGCGGC
  ATCAGGCCTACGAGTTCCAGTTTGGCGCAGCCTACGCTTGGATGATGTGCGTCTTCACGGTGGTCATGAC
  CTACAGTATCACCTGCCCCATCATCGTGCCCTTCGGGCTCATGTACATGCTGCTGAAGCACCTGGTAGAC
  AGGTACAATCTCTACTACGCCTACTTGCCGGCCAAACTGGACAAGAAGATCCACTCGGGGGCTGTGAACC
  AGGTGGTGGCTGCGCCCATCCTCTGCCTCTTCTGGTTGCTCTTCTTCTCCACCATGCGCACGGGTTTCCT
  AGCCCCCACATCTATGTTCACCTTCGTTGTTCTGGTAATCACCATCGTCATCTGTCTCTGCCACGTCTGC
  TTTGGACACTTCAAATACCTCAGTGCCCACAACTACAAGATTGAACACACAGAGACAGATGCCGTGAGCT
  CCAGAAGTAATGGACGGCCCCCCACTGCTGGCGCTGTCCCCAAATCTGCGAAATACATCGCTCAGGTGCT
  GCAGGACTCAGAGGGGGACGGGGACGGAGATGGGGCTCCTGGGAGCTCAGGCGATGAGCCCCCATCGTCC
  TCCTCCCAAGACGAGGAGTTGCTGATGCCTCCTGATGGCCTCACGGACACAGACTTCCAGTCATGCGAGG
  ACAGCCTCATAGAGAATGAGATTCACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208385 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MINPKPCGHLCCCVVRGCEQVEAIEYYTKLEQRLKEDYRREKEKVNEKPLGMAFVTFHNETITAIILKDF
  NVCKCQGCTCRGEPRASSCSEALHISNWTVTYAPDPQNIYWEHLSIRGFIWWLRCLVINVVLFILLFFLT
  TPAIIITTMDKFNVTKPVEYLNNPIITQFFPTLLLWCFSALLPTIVYYSAFFEAHWTRSGENRTTMHKCY
  TFLIFMVLLLPSLGLSSLDLFFRWLFDKKFLAEAAIRFECVFLPDNGAFFVNYVIASAFIGNAMDLLRIP
  GLLMYMIRLCLARSAAERRNVKRHQAYEFQFGAAYAWMMCVFTVVMTYSITCPIIVPFGLMYMLLKHLVD
  RYNLYYAYLPAKLDKKIHSGAVNQVVAAPILCLFWLLFFSTMRTGFLAPTSMFTFVVLVITIVICLCHVC
  FGHFKYLSAHNYKIEHTETDAVSSRSNGRPPTAGAVPKSAKYIAQVLQDSEGDGDGDGAPGSSGDEPPSS
  SSQDEELLMPPDGLTDTDFQSCEDSLIENEIHQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057136
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057136, AAH57136
• RefSeq Size: 1702 bp
• RefSeq ORF: 1571 bp
• Locus ID: 224807
• MW: 59.5 kDa