Rps6kb1 (NM_001114334) Mouse Tagged ORF Clone

CAT#: MR208420

Rps6kb1 (Myc-DDK-tagged) - Mouse ribosomal protein S6 kinase, polypeptide 1 (Rps6kb1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rps6kb1
• Synonyms: 2610318I15Rik; AA959758; AI256796; AI314060; p70 S6K-alpha; p70 S6KA; p70-S6K 1; p70/85s6k; p70s6k; P70S6K1; S6K; S6K-beta-1; S6K1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208420 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGACGACGGAGGCGGGACGGCTTTTACCTAGCGCCTGACTTCCGACACAGGGAAGCTGAGGACA
  TGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAAGATGCAGGCTCTGAGGATGAGCTGGAGGAGGG
  GGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCATGGAACATTGTGAG
  AAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTAC
  TTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATACTGG
  GAAGATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTGAGGAATGCTAAGGACACGGCCCACACG
  AAAGCAGAGCGGAACATTCTGGAGGAAGTGAAACACCCTTTCATTGTGGACCTGATTTATGCCTTTCAGA
  CCGGAGGGAAGCTCTACCTCATCCTCGAGTATCTCAGTGGAGGAGAACTATTTATGCAGTTAGAAAGAGA
  GGGAATATTCATGGAAGACACAGCGTGCTTTTACTTGGCTGAAATCTCCATGGCTTTGGGGCATTTACAT
  CAAAAAGGGATCATCTACAGAGACCTGAAGCCGGAGAACATCATGCTTAATCACCAAGGTCACGTGAAAC
  TAACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACAGTCACGCACACATTTTGTGGAACAAT
  AGAATACATGGCCCCTGAAATCTTAATGAGAAGCGGCCACAACCGTGCTGTGGATTGGTGGAGTTTGGGA
  GCATTAATGTATGACATGCTGACTGGAGCACCTCCATTCACTGGGGAGAATAGAAAGAAAACAATTGACA
  AAATCCTCAAATGTAAACTTAATTTGCCTCCCTACCTCACACAAGAAGCTCGAGATCTGCTTAAAAAGCT
  GCTGAAAAGAAATGCTGCTTCTCGTCTTGGAGCTGGCCCTGGGGATGCTGGAGAAGTCCAAGCTCATCCT
  TTTTTCAGACACATTAACTGGGAAGAACTTCTGGCTCGGAAGGTAGAACCTCCCTTTAAGCCTCTGTTGC
  AATCTGAAGAGGATGTGAGTCAGTTTGATTCAAAGTTTACACGTCAGACACCTGTTGACAGCCCTGATGA
  CTCCACTCTCAGTGAAAGTGCCAACCAGGTCTTTCTGGGTTTTACATATGTGGCTCCATCTGTACTTGAA
  AGTGTGAAAGAAAAGTTTTCATTTGAACCAAAAATCCGATCTCCTAGAAGATTTATTGGTAGTCCACGAA
  CACCTGTCAGCCCAGTCAAATTCTCTCCTGGGGATTTCTGGGGACGAGGTGCTTCAGCCAGCACGGCAAA
  TCCTCAGACCCCTGTGGAATACCCAATGGAAACAAGTGGGATAGAGCAGATGGATGTGACAGTGAGCGGG
  GAAGCATCAGCGCCACTTCCAATCCGACAACCCAACTCCGGGCCGTACAAAAAACAAGCTTTTCCTATGA
  TCTCCAAACGACCAGAGCACCTGCGGATGAATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208420 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRRRRRDGFYLAPDFRHREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCE
  KFEISETSVNRGPEKIRPECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHT
  KAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMEDTACFYLAEISMALGHLH
  QKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLG
  ALMYDMLTGAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHP
  FFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVDSPDDSTLSESANQVFLGFTYVAPSVLE
  SVKEKFSFEPKIRSPRRFIGSPRTPVSPVKFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTVSG
  EASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114334
• ORF Size: 1578 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114334.1, NP_001107806.1
• RefSeq Size: 5395 bp
• RefSeq ORF: 1578 bp
• Locus ID: 72508
• MW: 59.1 kDa